Lymphocyte blastogenic responses to inciting food allergens in dogs with food hypersensitivity

Lymphocyte blastogenic responses against food allergens in dogs with food hypersensitivity were evaluated in this study. Eleven dogs with food hypersensitivity, based on food elimination and oral food provocation tests and allergic responses to food allergens, were examined by various tests such as intradermal testing, antigen-specific IgE testing, and lymphocyte blastogenic responses. The number and kinds of food allergens identified as positive by these tests were compared with the offending food allergens that were found in an oral food provocation test. In 9 (82%) of the 11 dogs with food hypersensitivity, there was close agreement for positive allergens between the results of lymphocyte blastogenic responses and oral food provocation test; however, there was little agreement for intradermal and IgE testing of the positive allergens with those of the oral food provocation test (11% and 31%, respectively). In the 9 dogs, the stimulation indices of lymphocyte blastogenic responses increased to 2.0-10.1 upon food provocation but decreased significantly to 0.7-1.4 upon feeding the elimination diet until clinical signs disappeared. These results indicate that lymphocyte blastogenic responses may fluctuate because of exposure to offending food allergens in dogs with food hypersensitivity. Lymphocytes reactive to food allergens may play an important role in the pathogenesis of food hypersensitivity in dogs.     

补肾益寿胶囊药渣栽培鲍鱼菇及营养成分分析

用补肾益寿胶囊药渣、木屑为主要原料栽培鲍鱼菇,筛选最适栽培配方并测定鲍鱼菇的营养成分。结果表明,栽培鲍鱼菇的最佳配方是补肾益寿胶囊药渣40%、木屑40%、麦麸18%、石膏1%和蔗糖1%。此时,鲍鱼菇菌丝体的日均长速为0.34 cm/d,生物学效率为55.8%。鲍鱼菇碳水化合物、粗蛋白、粗纤维、灰分和粗脂肪的含量分别为48.80%、19.76%、8.12%、9.21%和13.25%。     

The Proteomic Research of the Cure of Experimental Diabetes Deafness by Granules of Eliminating Phlegm and Removing Blood Stasis

Obiective:Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB),screenin...     

黑纹粉蝶Pieris melete Ménétriès生殖系统

【目的】研究黑纹粉蝶Pieris melete Ménétriès生殖系统的解剖学结构,以期发现更多的分类特征,为其所属类群乃至粉蝶亚科的系统发育分析提供依据。【方法】于2009-2010年夏季分别在浙江临安和陕西秦岭采集黑纹粉蝶成虫,在MODEL SZX2-ILLK型显微镜下观察雌虫和雄虫内、外生殖器的解剖结构,运用QImaging Retiga 2000R CCD数码相机进行照相,用图像叠加软件Automontage进行图像合成,描述其特征。【结果】黑纹粉蝶雌虫外生殖器包括交配囊及其附属结构、导精管、表皮突和肛突;内生殖器包括1对卵巢、1对侧输卵管、1根中输卵管、受精囊、附腺以及外生殖腔。雄虫外生殖器包括阳具、抱握器及其支持结构;内生殖器包括1个睾丸、2条输精管、1对贮精囊、开口于阳茎基部的射精管和附腺。【结论】黑纹粉蝶的卵巢两侧不对称,一侧的4根卵巢管直接合并,另一侧则是相邻的2根卵巢管先分别聚成一支,然后2支再聚合,与中输卵管相接。     

6个沙果品种果实香气成分分析

分析6个沙果品种的果实香气,旨在为利用种间杂交选育优良的苹果新品种提供新的亲本。采用静态顶空和气相色谱-质谱联用技术,对‘泰山花红’、‘秋风蜜’、‘一窝蜂’、‘大花红’、‘小花红’、‘莱芜花红’6个沙果品种果实香气进行了测试。结果表明,‘泰山花红’与‘大花红’果实香气物质以醇类为主,分别占总成分含量的67.24%和79.27%;‘秋风蜜’、‘一窝蜂’、‘小花红’和‘莱芜花红’的果实香气成分以酯类为主,其含量分别占总含量的98.86%、94.62%、81.35%和73.56%,推测沙果果实香型也可能分醇香型和酯香型两种类型。     

BIFMAM7.1-2007《办公家具系统、部件和座椅中散发的挥发性化合物测试方法》标准解析

对BIFMA M7.1-2007《办公家具系统、部件和座椅中散发的挥发性化合物测试方法》标准进行了解读,概述了该标准的适用范围、试验原理和方法,以及该标准在实际应用中的局限性,提出了控制办公系统家具中甲醛和VOC污染物以提高办公场所空气质量的指导意见。     

猪繁殖与呼吸综合征病毒GP3/GP5/M真核表达载体的构建及其体液免疫应答

本试验旨在构建表达猪繁殖与呼吸综合征病毒(PRRSV)GP3、GP5和M蛋白的真核重组质粒。以PRRSV LN株为模板,采用PCR方法扩增出GP3、GP5、M基因片段,将扩增的GP5、M通过Linker序列串联成GP5-M,然后将GP3与GP5-M双酶切后插入pcDNA3.1(+)构建重组质粒pcDNA3.1-GP3-GP5-M,将其转染COS7细胞。PCR鉴定表明重组质粒pcDNA3.1-GP3-GP5-M含有PRRSV GP3、GP5-M基因,间接免疫荧光检测表明GP3、GP5-M蛋白在COS7细胞内获得表达。Western blotting检测证实GP3、GP5、M蛋白获得正确表达,并且所表达的GP3、GP5、M蛋白是融合蛋白。将pcDNA3.1-GP3-GP5-M免疫BALB/c小鼠,首免后2周可检测到特异性PRRSV中和抗体,首免后8周中和抗体效价最高可达1∶32。进一步将pcDNA3.1-GP3-GP5-M免疫断奶仔猪,首免后4周即可产生1∶4～1∶8的中和抗体。本试验成功构建了表达PRRSV GP3、GP5和M融合蛋白的真核重组质粒pcDNA3.1-GP3-GP5-M,中和抗体检测表明pcDNA3.1-GP3-GP5-M具有良好的免疫原性,从而为PRRSV基因工程疫苗的研制奠定基础。     

Influence of birth order,birth weight,colostrum and serum immunoglobulin G on neonatal piglet survival

Background

Intake of colostrum after birth is essential to stimulate intestinal growth and function, and to provide systemic immunological protection via absorption of Immunoglobulin G (IgG). The birth order and weight of 745 piglets (from 75 litters) were recorded during a one-week period of farrowing. Only pigs weighing greater than 0.68 kg birth weight were chosen for the trial. Sow colostrum was collected during parturition, and piglets were bled between 48 and 72 hours post-birth. Piglet serum IgG and colostral IgG concentrations were determined by radial immunodiffusion.

Results

Sow parity had a significant (P < 0.001) effect on sow colostral IgG concentration, being 5% higher in multiparous females. Sow colostral IgG concentration explained 6% and piglet birth order accounted for another 4% of the variation observed in piglet serum IgG concentration (P < 0.05); however, birth weight had no detectable effect. Piglet serum IgG concentration had both a linear (P < 0.05) and quadratic effect (P < 0.05) on % survival. Piglets with 1,000 mg/dl serum IgG or less (n=24) had a 67% survival; whereas, piglets with IgG concentrations between 2250 to 2500 mg/dl (n=247) had a 91% survival. Birth order had no detectable effect on survival, but birth weight had a positive linear effect (P < 0.05). Piglets weighing 0.9 kg (n = 107) at birth had a 68% survival rate, and those weighing 1.6 kg (n = 158) had an 89% survival.

Conclusion

We found that the combination of sow colostrum IgG concentration and birth order can account for 10% of the variation of piglet serum IgG concentration and that piglets with less than 1,000 mg/dl IgG serum concentration and weight of 0.9 kg at birth had low survival rate when compared to their larger siblings. The effective management of colostrum uptake in neonatal piglets in the first 24 hrs post-birth may potentially improve survival from birth to weaning.     

猪流行性腹泻病毒ORF3和M基因的克隆与序列分析

为了解福建省猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)毒株ORF3和M基因变异情况,本研究从4个不同规模猪场发生仔猪“顽固性腹泻”疾病的病料中扩增到4株PEDV的ORF3和M基因,并进行了序列分析.结果表明:4株PEDV ORF3基因均含有675个碱基,编码224个氨基酸,与GenBank中登录的代表性PEDV核苷酸的同源性为89.7％～100.0％,氨基酸的同源性为94.7％～99.6％,其中FJPT毒株核苷酸的同源性与CV777 truncated毒株最低,仅为89.7％,FJNP毒株核苷酸的同源性与中国北方流行的CH ZKFG 11毒株最高,达100.0％.M基因含有681个碱基,编码226个氨基酸,与GenBank中登录的代表性PEDV核苷酸的同源性为94.8％～100.0％,氨基酸的同源性为94.8％～99.6％,其中FJPT毒株核苷酸的同源性与日本D89752毒株最低,仅为94.8％,FJNP毒株核苷酸的同源性与韩国CPF193、泰国毒株最高,达100.0％.4株PEDV毒株均与国内外流行强毒株的亲缘关系较近,与attenuate DR13弱毒株的亲缘关系较远.     

Effect of phosphocreatine on transient outward potassium current in ischemic ventricular mid-myocardial cells of rats

AIM: To determine the effect of exogenous phosphocreatine (PCr) at different concentrations on transient outward potassium (I_to) current in rat ischemic ventricular mid-myocardial (M) cells and to explore the antiarrhythmia mechanism in the treatment of ischemic heart disease. METHODS: M cells were isolated enzymatically from left ventricular mid-myocardium of rats. Peak I_to current was recorded by patch-clamp technique in the whole-cell configuration when M cells were superfused with normal Tyrode solution, simple ischemic solution,and simulated ischemic solution containing PCr at concentrations of 5, 10, 20 and 30 mmol/L for 10 min. RESULTS: Peak I_to current density of M cells superfused with simple simulated ischemic solution was significantly reduced by (76.1?6.3)% (P<0.05) compared with M cells superfused with Tyrode solution. Ischemic solution containing 5, 10, 20 and 30 mmol/L PCr reduced peak I_to current density by (57.1?9.6)% (P<0.05), (40.3?10.3)% (P<0.05), (34.3?9.6)% (P<0.05) and (32.1?10.6)% (P<0.05),respectively. There was statistical difference among ischemic solution without PCr and containing PCr at concentrations of 5 and 10 mmol/L groups (P<0.05). No statistical difference among groups of 10, 20 and 30 mmol/L PCr was observed (P>0.05). CONCLUSION: PCr reverses the inhibition of I_to current under ischemic condition in M cells, which may be the mechanism responsible for arrhythmia prevention in ischemic heart disease. PCr at concentrations of 0~10 mmol/L exerts significant dose-effect relationship.