茶叶多糖的组合及免疫活性研究

用粗老茶制备要多糖（ＴＰＳ）的粗品（ＴＰＳ１）经脱蛋白处理过凝胶柱纯后得ＴＰＳ的纯品（ＴＰＳ２）,然后用ＵＶ、ＩＲ、ＨＰＬＣ、ＧＣ及常规成分分析等方法比较了ＴＰＳ１和ＴＰＳ２之间的组分差异;用ＡＡ（佐剂性关节炎）大鼠和正常小鼠为对象。比较ＴＰＳ１和ＴＰＳ２对动物免疫活性的影响。     

禽流感油乳剂灭活疫苗的研究

将6种不同亚型的禽流感病毒（AIVH2N9、H3N8、H5N1、H5N2、H7N1、H9N2）分别接种鸡胚,收获尿囊液,经甲醛灭活,以矿物油为佐剂制成油乳剂灭活疫苗。疫苗接种4和8周龄SPF鸡,注苗后均无不良反应。每种亚型疫苗免疫后14天和21天攻毒保护率均达90％-100％。分别用H5N1和H9N2亚型灭活疫苗免疫8周龄SPF鸡、25和28周龄健康商品蛋鸡,免疫后7天产生免疫力,14天保护率达100％,21天后抗体达高峰,仔鸡接苗后最高血凝抑制（HI）几何平均滴度（GMT）为7.3-8.0log2,蛋鸡为8.0-10.5log2。免疫后180天,抗体效价不低于6.5log2。免疫后180天分别以AIV攻击,H5亚型疫苗组,用强毒攻击无一发病和死亡,对照鸡全部发病死亡;H9亚型疫苗组,对照鸡在攻毒后72小时停产,免疫鸡无一发病且产蛋正常,对同源攻毒的保护率达100％。     

GnRH并列体二聚体主动免疫对雄兔血清睾酮的影响

用GnRH并列体二聚体主动免疫雄兔,并观察了免疫对雄兔血清睾酮与睾丸重量的影响。结果表明该方法能使雄兔血清睾酮水平降低,睾丸重量下降。     

Immunological properties of the SLLTEVET epitope of Influenza A virus in multiple display on filamentous M13 phage

Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages, such as M13, have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here, M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed, the hybrid phage was immunogenic and produced specific antibodies against M2e (2-9) in broiler chickens.     

瓜州县羊布病免疫试验推广与成效分析

瓜州县自2009年人间暴发布病以来,每年都有一定人和畜感染。2013年被省兽医局划定为布病防控一类区,连年采取“消毒、调运监管、检测、扑杀净化”的措施进行防控,但收效缓慢。2016年,按照《甘肃省羊布病区域免疫试点工作指导意见》开展了布病免疫试点工作,免疫接种取得了成功。之后羊布病免疫则纳入了动物防疫强制免疫病种,通过三年来免疫试验推广,取得了显著成效,人间布病发生率有所降低,畜间布病疫情得到了有效控制。     

Absence of protection from West Nile virus disease and adverse effects in red legged partridges after non-structural NS1 protein administration

The red-legged partridge (Alectoris rufa) is a competent host for West Nile virus (WNV) replication and highly susceptible to WNV disease. With the aim to assess in this species whether the inoculation of non-structural protein NS1 from WNV elicits a protective immune response against WNV infection, groups of partridges were inoculated with recombinant NS1 (NS1 group) or an unrelated recombinant protein (mock group), and challenged with infectious WNV. A third group received no inoculation prior to challenge (challenge group). The NS1 group failed to elicit detectable antibodies to NS1 while in the mock group a specific antibody response was observed. Moreover, no protection against WNV disease was observed in the NS1 group, but rather, it showed significantly higher viral RNA load and delayed neutralizing antibody response, and suffered a more severe clinical disease, which resulted in higher mortality. This adverse effect has not been observed before and warrants further investigations.     

家畜伊氏锥虫灭活苗制备及免疫研究观察

4种佐剂制备的伊氏锥虫灭活苗，通过小鼠免疫筛选试验，发现Ⅳ号佐剂苗安全性好，免疫原性强，ES抗原在免疫保护中起重要作用。用Ⅳ号佐剂制备的湖北、江苏、广西株伊氏锥虫灭活苗，通过小鼠和家兔2次免疫，对同株虫体的攻击小鼠分别获得20／20、12／12和20／20的保护，并耐受第2、第3次攻击，免疫期长达3－5个月以上。家兔获得7／8保护，体重明显增加，中和凝集抗体效价1：10－80倍。对照小鼠、家兔全部发病死亡。     

牛病毒性腹泻病毒弱毒活疫苗免疫持续期的研究

为检测牛病毒性腹泻病毒(BVDV)弱毒活疫苗在免疫牛体内抗体产生及其消长规律,评价弱毒疫苗的保护效力,并确定免疫持续期,本试验对免疫试验牛每头颈部肌肉接种BVDV SM株弱毒疫苗104.5TCID50,监测血清抗体效价,进行免疫持续期的确定。在疫苗免疫后的6个月、9个月和12个月,分别抽取5头免疫组和5头对照组牛,采用BVDV-JL强毒株进行攻毒试验,每头牛攻毒剂量为6×107.0TCID50/mL。结果显示疫苗免疫后12个月时血清中和抗体效价仍维持在1∶1048以上。攻毒结果显示,在3个不同时间点进行强毒攻击后,免疫组所有动物白细胞数量都没有下降也没有分离到病毒,而对照组动物白细胞数下降均超过30%,6个月和9个月时动物血清中均能分离到病毒,而12个月对照组动物由于年龄大,没有分离到病毒,因此暂定此疫苗的免疫持续期为9个月。     

GnRH-Ⅰ疫苗免疫效果影响因素、商业困境与免疫机理研究进展

哺乳动物中除豚鼠外GnRH-Ⅰ的主要序列和结构都是完全一致的，所以GnRH-Ⅰ疫苗的应用范围广。在动物去势及避孕和人类免疫治疗研究中显示了其无可比拟的优越性。本文综述了GnRH-Ⅰ疫苗的免疫机理、免疫效果的影响因素及其商业化的困境。     

强效艾美耳牌鸡球虫苗的免疫效果

在实验条件下,研究了强效艾美耳牌鸡球虫苗对平养鸡群的免疫效果。实验１比较了免疫鸡群和对照鸡群在接种后１、２、３、４、５、６周的肠道病变分,结果表明免疫鸡群较自然感染建立免疫的对照鸡群的免疫整齐度高,判断依据是免疫后２周盲肠细小、内容物亦少的眼观指标。实验２比较了免疫鸡群、不免疫不用药鸡群、不免疫用药鸡群在攻虫后的三处肠道病变,结果表明免疫鸡群对大剂量卵囊攻击有很强的抵抗力。实验３比较了免疫鸡群、不