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71.
72.
Atlantic salmon were fed 40, 300 or 1100 mgall-rac--tocopheryl acetate kg feed–1 for 12 weeks.After 6 weeks half of the fish in each group were marked and vaccinated (i.p.injection) against furunculosis and vibriosis. Liver -tocopherol levelsreflected the dietary input after 6 and 12 weeks of feeding. Noimmunomodulatoryeffects of dietary vitamin E on baseline levels of the immune parameters beforevaccination were detected in this study, as evaluated by antibody dependent andspontaneous plasma complement activities. In general, vaccination increasedplasma complement activities and the number of antigen specific antibodyproducing cells as compared with unvaccinated control fish, but with nodifferences with respect to the vitamin E regimes. Also the ability ofunvaccinated fish to withstand experimental furunculosis was unaffected bydietary vitamin E. The concentrations of -tocopherol and ascorbic acidinthe liver were, however, negatively affected 6 weeks post vaccination comparedto respective unvaccinated fish. 相似文献
73.
在32℃下对嗜水气单胸菌H20株作振荡培养,1%福尔马林、60℃灭活1hr.,制备白鲢暴发病菌苗。用该菌苗免疫白鲢,18-20℃水温下饲养10天后,白鲢血清中凝集抗体效价达1:1128左右,未免疫对照组凝集抗体效价约为1:8。免疫鱼的抗感染保护力达80%。 相似文献
74.
以梅花鹿茸为原料,采用超声波辅助法提取可溶性蛋白。以鹿茸可溶性蛋白得率为评价指标,通过单因素试验研究提取溶剂浓度、超声提取时间、超声提取功率和液料比对鹿茸可溶性蛋白得率的影响,在单因素试验的基础上进行正交试验,优化提取工艺条件。结果表明:鹿茸可溶性蛋白的最优提取工艺条件为,以pH=10.00、0.05 mol· L-1的碳酸盐缓冲溶液为提取溶剂,液料比10∶1,超声提取功率200 W,超声提取时间20 min。在此条件下,鹿茸可溶性蛋白得率为36.849 mg· g-1。体内免疫活性试验表明,鹿茸蛋白提取物能够提高免疫功能低下小鼠的单核巨噬细胞吞噬指数、血清溶菌酶质量浓度、血清及肝组织中酸性磷酸酶活力,具有良好的免疫活性。 相似文献
75.
The present study was to evaluate the effects of dried Allium mongolicum Regel (AMR) powder and its water- and fat-soluble extracts (AWE and AFE) on the growth performance, serum metabolites, immune responses, antioxidant status, and meat quality of lambs. A total of 32 male small-tailed Han lambs (5 months old; initial body weight = 34.8 ± 0.40 kg) were used in a 60-d feeding experiment after a 15-d adaptation period. The lambs were randomly divided into 4 groups (n = 8) and fed a basal diet (control, CON group), the basal diet supplemented with dried AMR powder at 10 g/d per lamb (AMR group), the basal diet supplemented with AWE at 3.4 g/d per lamb (AWE group), or the basal diet supplemented with AFE at 2.8 g/d per lamb (AFE group). Blood samples were collected on d 0, 30, and 60 in the feeding experiment (n = 8). At the end of the experiment, the lambs were sacrificed and the longissimus dorsi muscles collected. Growth performance was not significantly affected by dietary supplementation of AMR, AWE and AFE (P > 0.05). However, significantly lower albumin (P = 0.006), total protein (P = 0.006), globin (P = 0.025), and blood urea nitrogen (P = 0.024) concentrations were observed in AFE group relative to CON and AMR groups. Similarly, a significantly lower lactate dehydrogenase activity (P = 0.018) was observed in AFE group relative to AWE group, but not in other groups (P > 0.05). In addition, significantly increasing trends in glutathione peroxidase (P = 0.06) in AMR, AWE, and AFE groups were observed relative to the control group. Furthermore, significantly lower drip loss (P = 0.011) across the treatment groups and cooking loss (P = 0.048) were observed in the AMR group relative to the control group. Taken together, these results indicate that AMR and its extracts had no significant effect on lamb growth performance, antioxidant status, and immune responses, but could significantly improve meat quality without the occurrence of pathological kidney and liver lesions. 相似文献
76.
77.
肉用仔鸡主要营养、环境因素产量函数模型的研究 总被引:2,自引:1,他引:1
利用二次回归通用旋转组合设计对500只印第安河肉鸡生产的主要营养、环境因素进行了研究,建立了肉鸡生产函数模型,探讨了印第安河肉鸡日粮中粗蛋白质、代谢能水平及饲养密度等主要营养、环境因素及其相互效应和增重的关系,寻求了肉鸡增重最佳饲养管理措施组合方案和最优化的生产条件,为我国肉鸡生产技术的规范化提供了科学依据。肉鸡生产函数模型。y=2105.57+101.35X_1+42.93X_2-16.68X_3-16.3X_1X_2-99.47X_1~2-54.91X_2~2-51.16X_3~2。各因素对产量贡献大小顺序为粗蛋白质>代谢能>饲养密度。预测56日龄增重达1900克和净收入高于2.20元/只以上时,前期日粮需粗蛋白质21.55~22.37%,代谢能12.199~12.597MJ/kg,饲养密度32.9~34.4只/m~2;后期依次为19.55~19.76%,12.46~12.85MJ/kg,16.7~17.9只/m~2。蛋白质和代谢能存在交互作用。 相似文献
78.
79.
我国棚室覆盖材料的应用与发展(上) 总被引:10,自引:1,他引:9
通过对棚室覆盖材料的沿革,种类及功用,应用与发展,问题与对策等的论述,阐述了我国棚室覆盖材料的开发应用现状和研究发展方向。 相似文献
80.
Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) prevent development of T-helper type 2 (Th2) immune response and reverse established allergic responses in mouse models. However, little work on immune responses in piglets has been conducted in vivo. In this report, the ability of a porcine-specific CpG ODN to act as an immunostimulant and enhance immune responses of piglets to swine Pasteurella multocida living vaccine (SPML vaccine) was determined. The titre of IgG and IgG1/IgG2 isotype to SPML vaccine in serum, the proliferation of lymphocytes, SPML-specific interferon-gamma (IFN-gamma) and IL-6, TNF-alpha, IL-4 production of PBMCs in vitro and IFN-gamma, IL-6, TNF-alpha, IL-4, IL-10 in piglets serum were examined to identify the immune responses of the piglets. Immune responses of the piglets vaccinated with SPML and CpG ODN were significantly stronger than responses of piglets vaccinated with SPML alone. All these data summarized that immunostimulatory CpG ODN could modulate the immune response towards a Th1-like response when co-administered to piglets during SPML vaccination, which suggested that the therapeutic uses envisioned for these ODNs (as vaccine adjuvants and immunoprotective agents) may be applicable to husbandry animals. 相似文献