针叶中紫外吸收物质含量的测定

风干处理对常见针叶植物叶中紫外吸收物质含量没有影响,60℃水浴提取1h或浸泡7天的方法都能有效提取出针叶中的紫外吸收物质;针叶中紫外吸收物质的含量可用A300·L·g-1FW来表示。     

FAM3C activates Akt to promote viability of oral squamous-cell carcinoma cells

AIM: To investigate the expression and roles of family with sequence similarity 3, member C (FAM3C) in oral squamous-cell carcinoma cells. METHODS: The mRNA and protein expression levels of FAM3C in dysplastic oral keratinocyte (DOK) and oral squamous-cell carcinoma WSU-HN6 cells were detected by RT-qPCR and Western blot. The WSU-HN6 cells were treated with siFAM3C or FAM3C antibody. After 24, 48 and 72 h, the viability of WSU-HN6 cells was measured by CCK-8 assay, and the activation of protein kinase B (Akt) was detected by Western blot. Adenovirus was used to mediate over-expression of FAM3C in the DOK cells. The DOK cell viability was measured by CCK-8 assay after adenovirus infection for 24, 48 and 72 h, and the activation of Akt was detected by Western blot. RESULTS: Compared with the DOK cells, the mRNA and protein levels of FAM3C were significantly increased in the WSU-HN6 cells (P<0.05). The viability of WSU-HN6 cells transfected with siFAM3C was significantly inhibited at 48 h and 72 h (P<0.05). siFAM3C treatment inhibited the activation of Akt (P<0.05). FAM3C antibody treatment also suppressed the viability of the WSU-HN6 cells at 48 h and 72 h and the activation of Akt (P<0.05). Over-expression of FAM3C in the DOK cells promoted the cell viability at 48 h and 72 h and activated Akt (P<0.05). CONCLUSION: FAM3C might promote oral squamous-cell carcinoma cell growth by activating Akt.     

Efficacy of Soya Lecithin Powder Combined with Conventional Therapy on Chronic Hepatitis B

[Objectives]To probe into the treatment effects of soya lecithin powder combined with conventional therapy on chronic hepatitis B.[Methods]A total of 120 patien...     

Dexmedetomidine attenuates acute alcoholic hepatic injury in mice

AIM: To investigate the effects of dexmedetomidine (DEX) on acute alcoholic hepatic injury in mice and to explore the possible mechanisms. METHODS: Kunming mice (n=50) were randomly divided into 5 groups (n=10): normal saline control (NS) group, acute alcoholic hepatic injury model (E) group, low-dose (10 μg/kg) DEX (E+L) group, medium-dose (50 μg/kg) DEX (E+M) group and high-dose (100 μg/kg) DEX (E+H) group. The animals were sacrificed at 6 h after gavage of alcohol or normal saline. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were measured. The livers were removed for evaluation of histological characteristics and determining the content of tumor necrosis factor-α (TNF-α) amd interleukin-1β (IL-1β) in the liver tissues by ELISA. The expression levels of cytochrome P450 2E1 (CYP2E1) and nuclear factor-κB (NF-κB) in the liver tissues were evaluated by Western blot. RESULTS: Compared with NS group, the levels of ALT, AST and TG were obviously increased in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the levels of TNF-α, IL-1β and MDA were obviously increase in E group, which were obviously decreased in E+M and E+H groups. Compared with NS group, the activity of SOD and the content of GSH were obviously decreased in E group, which were obviously increased in E+M and E+H groups. Compared with NS group, the expression of CYP2E1 and NF-κB was obviously increase in E group, which was obviously decreased in E+M and E+H groups. Compared with NS group, ethanol induced marked liver histological injury, which was less pronounced in E+M and E+H groups. CONCLUSION: DEX has a protective effect on mouse liver with acute alcoholic injury by the involvement in the processes of antioxidation and antiinflammation, and its mechanism may be associated with the inhibition of CYP2E1 and NF-κB expression.     

TRMM在海河流域南系的降水估算精度评价及其对SWAT模型的适用性

准确估算区域降水对水文过程评价和水资源管理意义重大。为评估TRMM 3B42V7降水产品在海河流域南系的估算精度及其在土壤和水评估模型(Soil and Water Assessment Tool,SWAT)中的适用性,利用28个气象站降水观测数据(2007-2016年)和101个雨量站观测数据(2010-2016年)开展研究。研究表明:站点尺度上,3B42V7降水产品对月降水估算的均方根误差小于15 mm,平均误差小于8.5 mm;在湿润季节的估算精度更好。流域尺度上,日降水估算精度较差,相关系数小于0.6。分区尺度上,3B42V7能够很好地捕捉到不同等级降水强度,但对微量降雨有所低估;山区和平原的年降水量均出现高估现象,平原区较为突出;此外,3B42V7能够较好地捕捉到研究区内极端降水的时间和空间分布。分2种情景进行水文模拟,利用月平均流量对模型进行校准和验证,在情景Ⅰ中,验证期模拟结果较好,决定系数在0.56~0.96之间,纳什效率系数在-11.09~0.94之间。TRMM 3B42V7可为海河流域及其类似区域的水资源管理提供参考。     

Effect of hydrogen sulfide on myocardial fibrosis and PPARγ and NF-κB expression in rat model of diabetes

AIM: To explore the effects of hydrogen sulfide (H₂S) on the myocardial fibrosis in a rat model of diabetes and its mechanism.METHODS: Single intraperitoneal injection of streptozotocin (STZ) was utilized to establish a rat model of diabetes. Sodium hydrosulfide was used as an exogenous donor of hydrogen sulfide. Male SD rats were randomly divided into control group, STZ group, STZ+H₂S group and H₂S group. Eight weeks later, HE and VG staining methods were used to observe the collagen distribution and collagen volume fraction was measured by image analysis. The expression levels of type I collagen, PPARγ and NF-κB in the cardiac tissues were determined by Western blotting.RESULTS: Compared with control group, collagen distribution and the expression levels of type I collagen and NF-κB in the cardiac tissues were markedly increased (P<0.05), while PPARγ was significantly decreased in STZ group (P<0.05), but these indexes were reversed significantly in STZ+H₂S group (P<0.05). The expression levels of type I collagen, PPARγ and NF-κB had no significant difference between H₂S group and control group.CONCLUSION: Hydrogen sulfide attenuates cardiac fibrosis in diabetic rats, and its mechanism may be related to PPARγ-NF-κB signaling pathway.     

木质养殖船的稳性衡准

针对木质养殖船无建造规范可进行稳性衡准,对大连市庄河、长海县、普兰店、开发区部分养殖船进行实船测绘,并对163艘养殖船资料进行了统计与分析,从中总结出适合大连地区木质养殖船的稳性衡准计算方法.     

全球变暖背景下江淮地区气候变化趋势预估

利用政府间气候变化委员会（IPCC）提供的20多个最新一代气候模式的预测结果,分析了中等排放SRESA1B情景下我国江淮地区2011～2100年的气候变化趋势。结果表明,在全球变暖的背景下,未来江淮地区年平均气温将不断上升,增温幅度由东南向西北逐渐升高,到21世纪末年平均气温将上升3.3℃;年平均降水量持续增多,降水增幅随纬度的升高以及随时间的推进而加大,且将在2041年后显著增强。     

Lactic Acid Inhibits NF-κB Activation by Lipopolysaccharide in Rat Intestinal Mucosa Microvascular Endothelial Cells

To investigate whether lactic acid could inhibit the LPS-activation of NF-κB p65 in rat intestinal mucosa microvascular endothelial cells (RIMMVECs), RIMMVECs, cultured in vitro, were pretreated with different concentrations of lactic acid and then exposed to lipopolysaccharide (LPS). Cells and cell culture media were then collected at different time intervals. Production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) was examined at the protein level by enzyme-linked immunosorbent assay. The influence of lactic acid on the LPS-activation of NF-κB was examined at mRNA and protein levels by real-time quantitative PCR and Western blot analysis, respectively. TNF-α and IL-6 protein levels were significantly decreased after pretreatment with lactic acid compared with cells exposed to LPS only. After pretreatment with 7.5, 5.0, and 2.5 μL mL-1 lactic acid, NF-κB mRNA levels were increased by 1.51-, 2.62- and 3.00-fold, respectively, compared with levels in control cells without LPS treatment. Western blot analysis indicated that the level of NF-κB p65 in the lactic acidpretreated group was significantly lower than that in the group treated with LPS only (positive control) and was unchanged compared with the group without LPS treatment (blank control). These results suggest that lactic acid may inhibit LPSactivation of NF-κB, leading to the down-regulation of TNF-α and IL-6.     

丽蚜小蜂与黄板联合使用对设施番茄烟粉虱的控制作用研究

[目的]通过研究设施条件下人工释放丽蚜小蜂与黄板联合使用对番茄上烟粉虱的控制作用,探讨设施农业烟粉虱的生物防治技术.[方法]每株烟粉虱成虫不超过3头,若虫不超过2头左右释放,每5-7 d释放一次,连续释放5次,每次5 000头/667 m2.[结果]丽蚜小蜂平均出蜂率均在84.5;以上,最高寄生率达73;.从起始释放到其建立种群历时约35 d,约两个月后控制烟粉虱种群.丽蚜小蜂在番茄上的分布调查表明:垂直方向上,在番茄生长旺盛期,丽蚜小蜂主要分布于植株的中下部;而当番茄生长中后期,丽蚜小蜂随着烟粉虱分布的上移逐渐分布于植株中上部;水平分布由东向西(棚室进门向内)依次表现为高→低→高→低→高的规律性.[结论]人工释放丽蚜小蜂为主结合黄板诱杀对番茄上烟粉虱防治效果显著.黄板可有效地降低烟粉虱成虫数量而对丽蚜小蜂的诱杀作用很小.