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Two Chinese domestic yak populations representing the Plateau type and the Huanhu Alpine type were analysed with 12 bovine microsatellite primers. All primer pairs functioned in the yak genome and polymorphism was found at all loci. The allele size ranges and frequencies of the two yak populations were similar and there was considerable overlap with the allele size ranges observed in cattle. Data for European cattle breeds was obtained from the Cattle Diversity Database(CaDBase)to interpret the heterozygosity and genetic distance estimates in yak populations. Heterozygosity estimated for the two yak populations was comparable to that of European cattle while Nei's Genetic Distance DA between the two yak populations was less than distances between the most closely related German cattle breeds. Bovine microsatellite primers proved to be a valuable tool for characterization of yak populations. 相似文献
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互助白牦牛红细胞钾浓度多态性的研究 总被引:3,自引:0,他引:3
采用火焰光度计法对70头互助白牦牛的红细胞钾浓度多态性进行研究。结果表明:①被检白牦牛有高血钾和低血钾两种表型,以高血钾型为优势表型(85.71%);②K^h和K^L等位基因频率分别为0.9258和0.0742;③白牦牛红细胞钾浓度基因座的有效等位基因数、基因杂合度和基因均质度指数分别为1.1593,0.1374和0.752。 相似文献
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云南盐津乌鸡血液蛋白及同工酶遗传多态性研究 总被引:7,自引:1,他引:7
采用水平式淀粉凝胶电泳技术,对云南盐津乌鸡34只个体共计34个基因座位的血液蛋白及同工酶多态性进行研究,发现LAP,AKP-1,AKP-2,CKs-1,PEP-B5个座位具有多态性,多态座位百分比和平均杂合度分别为P=0.1470,H=0.0586.对多态座位基因频率进行计算发现盐津鸟鸡LAPB,aKP-1,AKP-2°,CEs-lA,PEP-BA频率较高.这些结果表明云南盐津乌鸡遗传多样性程度较常见的外国鸡种高,且具有独特基因型.属选育程度低,选择潜力大的地方品种,具有较高的保种价值. 相似文献
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Molecular characterization of breeds and its use in conservation 总被引:3,自引:0,他引:3
The conservation of farm animal resources is important for coping with future breeding needs and for facilitating the sustainable use of marginal areas. The increasing availability of molecular markers for most farm animal species and the development of techniques to analyse molecular variation is widening our capacity to characterise the genetic variation of breeds. In this paper we review the most popular molecular markers used in conservation and animal breeding studies, the different measures of genetic diversity that they provide, and their application for managing within-breed genetic diversity and for setting between-breed conservation priorities. We also address the relationship between genomic and marker heterozygosity, the relationship between molecular and quantitative measures of genetic diversity, and the characterization of breeds based on non-neutral markers. 相似文献
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猪微卫星座位杂合度与肌肉肌苷酸含量的关系 总被引:5,自引:2,他引:3
英系大白猪和梅山猪产生的139头F2代个体用作试验猪。分析猪1、2和6号染色体的24个微卫星座位与肌肉肌苷酸的关系,发现5个座位与肌苷酸含量显著关联(P<0 05)。它们分别是1号染色体的Sw2185、2号染色体的Sw2516、S0170、Sw1883和Sw2192。6号染色体9个微卫星座位均没有显著的相关(P>0 05)。这提示2号染色体的一些基因或QTL对肌苷酸含量有较大的遗传作用。分别以24个座位分类、以5个显著关联座位分类和以19个中性座位分类来研究基因杂合度与肌苷酸含量的关系,结果均表明随着基因杂合度增加,肌苷酸含量呈下降趋势。其中前两者分析结果大体一致。基因杂合度在0 5~0 8范围内,每增加0 05则肌苷酸含量下降幅度大约是群体肌苷酸均值的1 2%~7 5%。提示显著关联座位在肌苷酸的杂种优势中可能具有重要的遗传效应。同时,在应用微卫星标记估计基因杂合度与肌苷酸含量的关系时,尽可能选用显著关联座位,可以作到多快好省。 相似文献
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采用聚丙烯酰胺凝胶电泳法对55只引入乐都的海兰褐鸡血清白蛋白和运铁蛋白的多态性进行研究。结果发现:(1)被检海兰褐鸡血清白蛋白有ALB BB,ALB BC二种基因型,以ALB BB型为优势基因型(90.91%),ALB^B和ALB^C等位基因频率分别是0.9545和0.045;(2)运铁蛋白只有TF BB一种基因型;(3)ALB和TF两位点的平均基因杂合度(H)和有效等位基因数(Ne)分别为0.0 相似文献