棉花幼苗侧根发生的化学诱导研究 Ⅲ.Ca~(2+)对棉花幼苗侧根发生和根系IAA含量的影响研究初报

清水或 DPC 200ppm 溶液浸种培养的棉花幼苗在移栽时用含 Ca~(2+)30ppm 的 CaCl_2溶液漫根2小时处理,栽后8天观察发现,两种浸种方式,Ca~(2+)均能促进棉苗侧根的发生,并提高根系 IAA 含量。但 Ca~(2+)处理 DPC 降低根对棉酚含量的效应无明显影响。上述试验结果显示出 Ca~(2+)与诱导棉苗侧根发生和调节根系 IAA 水平之间存在着某些内在的联系,这可能涉及到 Ca~(2+)·CaM系统(胞内第二信使系统)与植物激素效应之间的关系。     

桉树组培苗嫩梢扦插育苗专用生根粉的研究

采用两种植物生长调节剂对桉树组培苗嫩梢扦插育苗的试验表明，吲哚丁酸配制的生根粉对桉树嫩梢扦插的插条具有促进生根作用，且其Ｉ４号生根粉对各种桉树的嫩梢插条的促根作用更中明显，在试验的４种桉树无性系组培苗中，插条生根率都达到８０％以上。     

基于颠茄发根的外源基因表达系统的建立

 用携带植物高效表达载体pCAMBIA1304 ⁺ “解除武装”的重组C58C1工程菌转化颠茄无菌苗真叶, 发根诱导率达100%。PCR检测证实发根型质粒pRiA4和表达型质粒pCAMBIA1304 ⁺均能整合到颠茄发根基因组内, 共转化率达30%。建立了基于颠茄发根的高效外源基因表达系统, 为将托品烷类生物碱东莨菪碱的生物合成关键酶基因导入颠茄, 实现其次生代谢工程及开展分子育种奠定了基础。     

马铃薯试管薯诱导体系研究

为了加快马铃薯试管薯的繁殖速度,通过优化马铃薯试管薯诱导的影响因素,建立适合马铃薯试管薯诱导体系。结果表明：适合马铃薯试管薯诱导的培养基为MS+IBA 0.05 mg/L+NAA 0.05 mg/L+香豆素10 mg/L+蔗糖10%+活性炭0.2%,培养条件为暗培养,培养温度为20℃,可使结薯率达94.25%,单粒薯重达0.128 g,优质薯率达100%,为试管薯的工厂化生产奠定技术基础。     

基于Hydrus-1D模型的冬小麦根系层水分渗漏分析

利用Hydrus-1D模型模拟了河北省石津灌区冬小麦水分渗漏情况。结果表明,研究区40％的灌溉降雨量和38％的总供水量流出了200cm边界层,通过设置12种方案得到初始饱和度、2次春灌水量是影响灌区渗漏的主要因素,而二水时间间隔和第一水灌水日期影响相对较小。通过设置426种方案,分别得到了不同初始饱和度时,2次春灌灌水...     

Root exudation of prometryn and its metabolites from Chinese celery (Apium graveolens)

Root exudates from Chinese celery (Apium graveolens) and Chinese cabbage (pak choi, Brassica chinensis) plants treated by prometryn, an herbicide, were qualitatively and quantitatively investigated and compared under hydroponic cultivation. Prometryn and its metabolites released into the nutrient solution were analyzed by ultra-performance liquid chromatograph coupled with orbitrap mass spectrometer to investigate whether this xylem-mobile herbicide is exuded from the roots. The results showed that celery and pak choi had different root exudation profiles. Celery metabolized prometryn to prometryn sulfoxide and released both compounds from the roots. In contrast, pak choi barely metabolized or actively released prometryn from the roots. The concentration of prometryn sulfoxide released from celery after 96 hr was 21 µg/L, which was nearly one-third that of released prometryn. Our results indicate that the root exudation and translocation of xylem-mobile herbicides could be significant in plants and are highly species dependent compared with phloem-mobile herbicides.     

培养基中蔗糖浓度对甘薯茎尖诱导成苗的影响

蔗糖浓度在 0～ 5 0 mg/g的范围内 ,甘薯茎尖分生组织培养均可成苗。随着蔗糖浓度的增加 ,诱导成苗率呈钟形曲线。最适范围为 2 0～ 30 mg/g,在此范围内甘薯茎尖分生组织诱导成苗率达 80 %以上     

Drought responses of above‐ground and below‐ground characteristics in warm‐season turfgrass

Water shortages have become more chronic as periodic droughts prolong and water demand for urban and agricultural use increases. Plant drought responses involve coordinated mechanisms in both above‐ and below‐ground systems, yet most studies lack comparisons of root and canopy responses under water scarcity and recovery. This is particularly true of research focused on warm‐season turfgrasses in sandy soils with extremely low water holding capacity. To address the lack of examination of coordinated stress and recovery responses, this study compared the above‐ and below‐ground plant responses during a dry‐down period of 21 days and recovery among four warm‐season turfgrass species in the field. Canopy drought responses and recovery were quantified using digital image analysis. In situ root images were captured using a minirhizotron camera system. Common bermudagrass [Cynodon dactylon (L.) Pers.] endured the entire drought period without losing 50% green cover while other species lost 50% green cover in 11–34 days predicted from the regression. The interspecific differences in drought resistance were mainly due to root characteristics. Other drought mechanisms appear to be responsible for differences identified in drought resistance between “Zeon” and “Taccoa Green” manilagrass [Zoysia matrella (L.) Merr.]. Recovery was delayed for up to 2 weeks in the second year, warranting further evaluation for turfgrass persistence under long‐term drought. Three‐week drought posed no threat to the survival of zoysiagrass. Species and genotypic variations were found in achieving full post‐recovery, which can be used to develop water conservation strategies and to adjust consumer expectations.     

燕麦与毛苕子混播试验初报

通过对比试验的方法，进行了燕麦与毛苕子混播试验，结果表明：B处理鲜草产量明显高于其它处理，其抗倒伏性能随毛苕子播量的增加而降低。B处理的燕麦与毛苕子植株增高幅度也最大。