Canine T‐Zone Lymphoma: Unique Immunophenotypic Features,Outcome, and Population Characteristics

Background

Canine T‐cell lymphoma (TCL) is clinically and histologically heterogeneous with some forms, such as T‐zone lymphoma (TZL), having an indolent course. Immunophenotyping is an important tool in the classification of TCL in people, and can be equally useful in dogs.

Hypothesis/Objectives

We hypothesized that loss of expression of the CD45 antigen is a specific diagnostic feature of TZL.

Animals

Twenty dogs with concurrent histology and immunophenotyping by flow cytometry were studied in depth. An additional 494 dogs diagnosed by immunophenotyping were used to characterize the population of dogs with this disease.

Methods

Lymph node biopsies from 35 dogs with TCL were classified by 2 pathologists using WHO criteria. Twenty lymph nodes were from dogs with CD45− TCL and 15 were from CD45+ TCL. The pathologists were blinded to the flow cytometry findings. Outcome information was sought for the 20 dogs with CD45− lymphoma, and population characteristics of the additional 494 dogs were described.

Results

All 20 CD45− cases were classified as TZL. The 15 CD45+ cases were classified as aggressive TCL and are described in an accompanying paper. TZL cases had a median survival of 637 days. Examination of 494 additional dogs diagnosed with TZL by immunophenotyping demonstrated that 40% of cases are in Golden Retrievers, are diagnosed at a median age of 10 years, and the majority have lymphadenopathy and lymphocytosis.

Conclusions

TZL has unique immunophenotypic features that can be used for diagnosis.     

Growth and survival of juvenile seahorse Hippocampus abdominalis reared on live, frozen and artificial foods

The culture of seahorses has usually relied on cultured live foods or collected wild live foods as feed. This can be expensive in the case of culturing live foods and unreliable in the case of harvesting wild live foods. This investigation examined whether juvenile Hippocampus abdominalis from 0 to 2 months of age could be experimentally weaned onto frozen (Cyclop-eeze copepods) and artificial (Golden Pearls) food over 30-day periods with Artemia as a live food control, with 0-, 5-, 10-, and 20-day mixed feeding periods. Newborn juveniles could not be weaned onto Golden Pearls, with almost complete mortality. One-month-old juveniles could be weaned onto Cyclop-eeze with no effect on survival but with lower growth. One-month-old juveniles could be weaned onto Golden Pearls but with lower survival and lower growth. Two-month-old juveniles could be weaned onto Cyclop-eeze but with lower survival and lower growth. Two-month-old juveniles could be weaned onto Golden Pearls with no effect on survival but with lower growth. In all experiments, the rate of feeding strikes on Cyclop-eeze and Golden Pearls was lower than that on Artemia (e.g. range of mean 0.16–0.97 vs. 2.2–2.76 feeding strikes/min), both when Artemia were the sole food presented and when Artemia were cofed with the nonlive foods. However, mixed feeding Artemia with nonlive foods did appear to increase the rate of feeding strikes on nonlive foods. These results indicate the potential for incorporating more cost effective and reliable nonlive foods into seahorse culture.     

基于被动式红外触发相机的红腹锦鸡研究

为了制定更好的区域性保护策略,利用被动式红外触发相机对长青自然保护区内的红腹锦鸡(Chrysolophus pictus)进行了研究。结果表明,红腹锦鸡对研究区域生境具有选择性,在日活动时间分配上也有所差异。与同域分布的其它雉类相比,红腹锦鸡在研究区域内分布范围最广,限制其进行生境选择的主要因素为海拔和森林起源类型。具体表现为:主要活动于海拔1 200~2 000m的范围内,不利用海拔高于2 700m的区域,随着海拔的升高其相对丰度呈递减趋势;在栖息范围内喜欢在人工林及次生林中活动,较少利用天然林;典型的日间活动动物,一天中有两个高峰活动时段,分别为12:00—14:00,16:00—18:00。     

非硫法人工烘制金黄葡萄干工艺的研究

非硫法人工烘制金黄葡萄干，工艺流程短，产品不褐变，不含残硫，试验结果认为，配方Ａ处理液，处理温度８５℃，处理时间１５ｍｉｎ，烘干温度６５℃为非硫法人工烘制金黄葡萄干的最佳生产工艺参数。     

Exploration of the Biopolitics of GMOs： Using Golden Rice as an Analytical Model

Several disputes exist around Genetically Modified Organisms （GMOs）. This article uses the concept of biopolitics to refer to all the GMO-related political issues and the mechanisms that are used to handle them. As a world famous genetically modified crop developed for the welfare of humanity by public institutions, Golden Rice has on one hand won glories, whereas on the other met with criticisms. It could be used as an analytical model to illustrate the biopolitics of GMOs. On the basis of an overview of its technological background, this article first introduces the participants and the debated issues of the Golden Rice project and then the disputes between the supporters and opponents and consequently analyzes the biopolitics of the Golden Rice. In conclusion, this article justifies the biopolitics of the GMOs and its doctrine.     

影响金叶珊瑚朴嫁接成活率的因素

以金叶珊瑚朴为接穗,普通珊瑚朴为砧木,进行了嫁接繁殖研究.试验结果表明:接穗粗度、砧木苗龄、嫁接时间、嫁接方法、保湿措施对嫁接成活率均有不同程度的影响.因接穗粗度不同而引起的成活率差异最大,达到100%;因砧木苗龄不同而引起的成活率达到78.7%,不同嫁接时间的成活率差达到76.2%,不同嫁接方法的成活率差达到56.2%,不同保湿措施的成活率差达到56.2%.选用健壮的两年生珊瑚朴实生苗作砧木,以金叶珊瑚朴充实的休眠枝为接穗,在早春砧木刚萌动时进行枝接并用塑料袋保湿,成活率最高,达到100%.     

紫金山风景林景观格局变化的研究

根据风景林的美学特征,风景林划分为水平郁闭型、垂直郁闭型、稀疏型、空旷型、园林型5种类型.在风景林类型划分的基础上,以南京紫金山风景区1988年和2002年两期森林资源二类调查成果为主要信息源,在桌面地理信息系统软件Mapinfo7.8的支持下,提取斑块平均面积、斑块平均周长、分维数、斑块形状指数和景观破碎度等景观格局指数,采用统一样点取样法,借助MapBasic提供的创建图形对象函数功能,构造出转移概率矩阵,代入Markov模型,从而对紫金山风景区1988-2002年间的景观格局及其变化趋势进行了全面的分析;在对计算结果进行分析的基础上,对紫金山风景林经营提出了一些建议和对策.     

金光杏梅果实生长动态观察初报

研究结果表明,金光杏梅果实生长发育动态呈“慢-快-慢-快-慢”的“3S”型曲线．整个发育过程可分为5个时期:幼果缓慢生长期、果实第一次迅速生长期、果实第二次缓慢生长期、果实第二次迅速生长期和果实熟前缓慢生长期。果实第二次缓慢生长期与果实硬核期相吻合,果实第一次迅速生长期和第二次迅速生长期是果实增长的两个关键时期。果实纵、横、侧径和果肉厚度与果实鲜重和体积变化曲线极为相似,经相关分析,果实纵、横、侧径和果肉厚度与果实鲜重的相关系数分别为0．9421**、0．9442**、0．9413**和0．9843**,与果实体积的相关系数分别为0．9391**、0．9462**、0．9373**和0．9824**,均呈极显著相关,说明它们是同步增长的。果核的生长发育动态呈“快-慢-停”的近“厂”型曲线,谢花后31d以前是迅速生长期;31d后进入缓慢生长期并开始硬化、变色:至59d达到固有大小和颜色,硬化结束,59d后停止生长。     

Design of microirrigation laterals on nonuniform slopes

A method for designing microirrigation laterals on nonuniform slopes was developed using the finite element method. Six representative nonuniform slope patterns were discussed in detail. The design principle was implemented based on the results of computer simulations. It was found that a single lateral is suitable for Pattern I while paired laterals are better for Patterns II ∼ VI in most cases. The diameter of a single lateral or paired laterals may have two solutions for a required uniformity of water application and the length may have multiple solutions. When the required average emitter discharge, required uniformity of water application, and one parameter (either length or diameter) of a single lateral or paired laterals are given, the unknown parameter, best submain position (paired laterals) and operating pressure head can be accurately designed using personal computers. The design procedures are described. Received: 2 November 1995     

金针菇多糖研制功能性酸奶的研究

[目的]探讨生产新型金针菇多糖功能性酸奶的方法。[方法]在前期研究的基础上,以所提取的水溶性金针菇粗多糖为功能性辅料,以鲜奶为主要原料,研制金针菇多糖酸乳,通过各项试验,确定复合稳定剂的最优配方以及酸奶最优生产工艺参数。[结果]结果表明,复合稳定剂的最优配方为0．03％黄原胶、0．01％明胶和0．01％羧甲基纤维素钠。酸奶最优生产工艺参数为金针菇粗多糖加入量0.8％、白砂糖加入量5．0％、发酵时间为6h,获得酸奶感官评分为95分。[结论]该研究为金针菇的深加工开辟一条新途径。