红掌愈伤组织辐射诱变及突变体的筛选

利用~(60)Co-γ射线对红掌‘阿拉巴马’的愈伤组织进行辐射,根据再生植株的叶色和花色变化筛选出突变体,并对突变体与亲本进行形态学、细胞学及分子水平的差异分析。结果表明:红掌‘阿拉巴马’愈伤组织的半致死剂量接近30Gy。当辐射剂量为30Gy时,再生植株M_1代、M_2代的性状一致,染色体数目与亲本一致2n=2x=30,仅有RAPD-751引物(序列:CCCACCACAC)条带与亲本有差异,并且能够稳定遗传,从而成功诱变出红掌突变体,这对提高红掌的育种效率具有重要的理论和实践意义。     

~(60)Co-γ射线辐照对桂花枝条生长和生理指标的影响及耐辐照性评价

为研究不同桂花扦插枝条的辐照诱变作用及其耐辐照性,以~(60)Co-γ射线对柳叶苏桂(金桂)、波叶银桂(银桂)和满条红桂(丹桂)的扦插枝条进行辐照,辐照剂量分别为15、25、35、45 Gy,计算生根数量和新根长度,检测新生叶的可溶性糖、可溶性蛋白质含量和遗传物质DNA,并计算桂花枝条半致死剂量(LD_(50))和综合评价值D。结果表明,3个桂花品种的根形态特征、新生叶的可溶性糖和可溶性蛋白质含量与辐照剂量均呈负相关;辐照引起新生叶遗传物质DNA的改变;柳叶苏桂、波叶银桂和满条红桂的综合评价值D分别为0.696、0.636和0.011,LD_(50)分别为39.77、32.19和27.96 Gy,表明金桂耐辐照性最强,银桂其次,丹桂最弱。本研究结果为桂花辐照育种适宜剂量的选择提供了依据,也为桂花不同品种群的辐照育种奠定了基础。     

60Co-γ射线辐射对云烟87、红花大金元种子活力的影响

采用不同辐射剂量的⁶⁰Co-γ射线辐射处理云烟87和红花大金元2个烤烟品种的种子,研究⁶⁰Co-γ射线辐射对云烟87、红花大金元种子活力的影响。结果表明,在25℃条件下,10～300 Gy辐射剂量处理对2个品种的种子活力都具有一定的促进作用,其中10 Gy低辐射剂量为最适辐射剂量;300 Gy以上的辐射剂量对种子发芽起抑制作用。同时也证明云烟87较红花大金元对辐射的适应性好,能更好地适应辐射处理来增强种子的活力。     

^60Co-γ射线对不同柑橘品种的辐射效应

以稻叶温州蜜柑（Citrus unshiu var.Inaba）、福橘（C.reticualta var.Fuju）、玫瑰橙（C.sinensis var.Meiguicheng）、华红（C.sinensis var.Huahong Newhall）和早金（C.sinensis var.Earlygold）等5个柑橘品种的接穗为试材,进行^60Co-γ射线照射处理,结合常规压片法,研究^60Co-γ射线对不同柑橘品种接穗的嫁接成活率及细胞有丝分裂行为的影响。结果表明：辐射累积剂量为2000R时,接穗嫁接成活率因品种不同而异,其中稻叶温州蜜柑的嫁接成活率最高（69.89%）,福橘次之,橙类较低（华红最低为32%）;^60Co-γ射线处理可诱发细胞有丝分裂异常,其诱变效应因柑橘品种不同而有所差别,柑类与橘类的有丝分裂异常率明显高于橙类。     

中国防沙治沙60年

根据60年来我国防沙治沙的发展实际,将我国的防沙治沙分为新中国成立初至70年代中期、70年代中期至90年代末期和新世纪3个阶段,分别对不同阶段的有关防沙治沙的环境背景、防沙治沙的开展情况、防沙治沙的成就进行简要的分析和总结,并从历史和现实的角度分别对某些社会关注或有争议的问题客观地表述了作者的看法。     

Resistance of Hard Tick （Ixodidae） with some Acaricide in Cattle （Naturally Infestation） in Sulaimani Governorate Fields-Kurdistan Regional/Iraq

A study carried out for assessment of acaricide for control and how to show resistance of hard tick （Ixodidae） with some types of acaricide which available in Sulaimani marked froro February to April 2012, isolated 80 cattle （local breed） naturally infested with hard tick. Six hundred and fifteen ticks min7 tick/cattle including three genera species were collected and identified, the highest Boophilus spp. followed by Hyalomma spp., and Rhipicephalus spp. were less frequent species collected. Experimental cattle divided into four groups each group content 20 cattle, treatment with four types acaricide and their average of infestation in cattle with Boophilus spp. 33 （41.25%） as the commonest, followed by Hyalomma spp. 31 （75.38%）, Rhipicephalus spp. 11 （13.75%） and 5 （6.25%） found mixed infested with Boophilus spp. and Hyalomma spp. 65 （81.3%） of cattle samples had emaciation and skin lesions. According to site of infestation, it showed that inguinal region was the most common predilection site for the ticks. Observed the genera Boophilus spp., there were highly significant differences （12.52%） between different tick species when calculated by general test （LSD）. The objective of this study was to estimate and compare these acaricide to control hard tick, and there was highly significant relation between different species of hard ticks when treated by these acaricide, according to the chi-square tests. The activities of the acaricide through the time of application were different on the tick genera species. Diazinon （60%） and carbamate （saven 85%） has been a greater degree than other acaricide resistance, while shows the activity of both acaricide injectable ivermactin and cypromethrein through the early time on all species. The results of the study otherwise provide encouraging possibilities for the potential use recommended dose with both acaricide ivermactin-l% and cypromethrine-10%.     

香蕉新株系"漳蕉8号"的选育

“台湾北蕉”离体试管芽经^(60)Co γ射线辐照后所培育的试管苗,在苗圃培育长出新叶后直到大田栽培期间都可观察到大量的变异植株。1995-1999年对28个优良变异单株进行筛选、株系比较试验和多点生产示范试验。结果表明：8号株系的假茎颜色、叶柄长度、株型以及果轴茸毛等性状与“台湾北蕉”有明显区别,且果穗外观较好,果指较长、较重;8号株系株产比“台湾北蕉”增加10％以上(差异达显或极显水平),果实品质略优于“台湾北蕉”。该株系现定名为“漳蕉8号”。     

A simple and rapid method for isolation of caliciviruses from liver of infected rabbits

Rabbit Haemorrhagic Disease Virus (RHDV), a member of the Caliciviridae family, is the etiologic agent of Rabbit Haemorrhagic Disease (RHD); this viral disease is highly contagious and kills more than 90% of infected adult rabbits. Research on experimental calicivirus infection uses inocula obtained from livers of rabbits dying from calicivirus infection. This implies that caliciviruses have to be purified from liver homogenates. Current methods to isolate caliciviruses from rabbit livers are time consuming. We propose here a new procedure for fast purification of rabbit caliciviruses from liver homogenates that uses centrifugation through an iodixanol gradient. This method offers in approximately 2 h a sample with a high degree of calicivirus purity, as shown by its biochemical and immunocytochemistry analysis, which is also able to kill adult rabbits from RHD within 48 h of inoculation.     

切花月季^60Co γ辐照诱变育种初报

以10个切花月季品种为材料,用60Co γ（30、40、50和60Gy）辐照接芽,研究辐照剂量对切花月季嫁接成活率及变异率的影响。结果表明：10个切花月季品种的嫁接成活率随辐照剂量的增加而降低;不同品种对辐照的反应不一样,仅有4个品种产生了可成活的变异,品种变异率高达3.06%～8.82%;变异主要发生在花型、花色、叶...     

靶向GP5基因脱氧核酶抑制PRRSV在MARC-145细胞中的复制

针对猪繁殖与呼吸综合征病毒（PRRSV）JL/07/SW株GP5基因设计了4个脱氧核酶,经体外切割试验筛选得到具有体外切割活性的脱氧核酶,并通过RT-PCR、TCID50、CPE、间接免疫荧光（IFA）检测具有切割活性的脱氧核酶抑制PRRSV复制效果进行评价。结果针对GP5基因的Dz-GP5-10抑制效率最高可达到82.4%,Dz-GP5-12也表现良好的抑制效果,表明GP5基因的这2个位点可能是PRRSV复制所必需的。本研究为PRRSV复制及基因组功能研究、抗病毒药物开发和转基因动物研究奠定了基础。