微生物降解1,2,4-三氯苯研究进展

1,2,4-三氯苯(1,2,4-TCB)的微生物降解机制包括好氧降解、还原脱氯和共代谢。本文简要综述了国内外对1,2,4-TCB微生物降解方面的研究进展,分析了其微生物降解的可行性,讨论了其好氧降解的微生物种类以及降解途径,阐述了厌氧条件下,混合菌群以及纯菌株对1,2,4-TCB还原脱氯的过程,目的是为1,2,4-TCB污染土壤的微生物修复提供依据。     

Carbon isotopic ratios of wetland and terrace soil sequences in the Maya Lowlands of Belize and Guatemala

This article provides new data and synthesizes earlier findings on the carbon isotope ratios of the humin part of soil organic matter from a range of sites in the central Maya Lowlands. Changes down the soil profile in carbon isotope ratios can provide an important line of evidence for vegetation change and erosion over time, especially in well dated aggrading profiles. Research thus far has provided substantial evidence for significant inputs from C₄ vegetation in buried layers from the Ancient Maya periods in depositional soils but equivocal evidence from sloping soils. We present new findings from soil profiles through ancient Maya wetland fields, upland karst wetlands, ancient Maya aguadas (reservoirs), and ancient Maya terraces. Most of the profiles exhibited δ¹³C enrichment greater than the 2.5–3‰ typical from bacterial fractionation. Seven of nine ancient Maya wetland profiles showed δ¹³C enrichment ranging from 4.25 to 8.56‰ in ancient Maya-dated sediments that also contained phytolith and pollen evidence of grass (C₄ species) dominance. Upland karst sinks and ancient reservoirs produced more modest results for δ¹³C enrichment. These seasonal wetland profiles exhibited δ¹³C enrichment ranging from 1 to 7.3‰ from the surface to ancient Maya-period sediments. Agricultural terraces produced mixed results, with two terraces having substantial δ¹³C enrichment of 5.34 and 5.66‰ and two producing only equivocal results of 1.88 and 3.03‰ from modern topsoils to Maya Classic-period buried soils. Altogether, these findings indicate that C₄ plants made up c. 25% of the vegetation at our sites in the Maya Classic period and only a few percent today. These findings advance the small corpus of studies from ancient terraces, karst sinks, and ancient wetland fields by demonstrating substantial δ¹³C and thus C₄ plant enrichment in soil profile sections dated to ancient Maya times. These studies are also providing a new line of evidence about local and regional soil and ecological change in this region of widespread environmental change in the Late Holocene.     

大鲵致病性嗜水气单胞菌SYBR GreenⅠ荧光定量PCR诊断试剂盒的研制

根据GenBank中致病性嗜水气单胞菌特异性的气溶素基因序列,设计1对引物,利用普通PCR技术扩增获得hlyA基因片段,并克隆到pMD-18T载体上作为阳性标准品。通过对SYBR GreenⅠ荧光定量PCR反应条件的优化,建立了快速检测致病性嗜水气单胞菌的SYBR GreenⅠ荧光定量诊断方法,以此为基础研制出试剂盒。试剂盒扩增产物的熔解曲线分析只出现1个单特异峰,无引物二聚体,对非致病性嗜水气单胞菌、弗氏柠檬酸杆菌、迟缓爱德华菌、柱状黄杆菌均无阳性信号扩增,重复性好,灵敏度可达1.0x101拷贝/uL。结果表明研制的致病性嗜水气单胞菌SYBR GreenⅠ实时荧光定量PCR试剂盒具有特异、灵敏、快速、重复性好等特点,适合于大鲵临床样品的检测。     

农村面源污染治理的“4R”理论与工程实践——案例分析

农村面源污染问题日益突出,是当前水污染控制与水环境改善的重点和难点。“十一五”期间,在国家水专项的支持下,以农村面源污染治理的“4R”理论（源头减量-过程阻断-养分再利用-生态修复）为指导,选择江苏省无锡市的直湖港小流域龙延村为综合示范区进行了技术的集成与工程化应用,取得了良好效果。本文以此为典型案例,详细介绍了综合示范区的污染现状、“4R”技术的集成应用与衔接配套、各项技术应用的污染控制效果以及区域环境改善效果,并对“4R”理论的核心内涵以及未来的发展进行了总结和讨论。     

太湖地区不同水旱轮作方式下稻季甲烷和氧化亚氮排放研究

为准确编制我国稻田温室气体排放清单及制定合理减排措施提供基础数据,选择太湖地区典型水稻种植区江苏省苏州市,研究设计了休闲水稻(对照,CK)、紫云英水稻(T1)、黑麦草水稻(T2)、小麦水稻(T3)和油菜水稻(T4)5种水旱轮作方式,采用静态箱气相色谱法,开展了不同水旱轮作方式下水稻生长季田间甲烷(CH4)和氧化亚氮(N2O)排放监测试验。试验结果表明:不同水旱轮作方式下水稻生长季CH4排放通量呈先升高后降低的变化趋势,CH4排放峰值出现在水稻生育前期,移栽至有效分蘖临界叶龄期CH4累积排放量占全生育期排放总量的比例为65%~81%,而N2O仅在水稻烤田期间有明显排放。水旱轮作方式对稻季CH4和N2O排放有极显著(P 0.01)影响,CH4季节总排放量表现为T1(283.2 kg.hm 2)CK(139.5 kg.hm 2)T3(123.4kg.hm 2)T4(114.7 kg.hm 2)T2(100.8 kg.hm 2),N2O季节总排放量顺序为T1 T4 T3 T2 CK,依次为1.06kg.hm 2、0.87 kg.hm 2、0.81 kg.hm 2、0.72 kg.hm 2和0.53 kg.hm 2。T1处理稻季排放CH4和N2O产生的增温潜势最高[7 396 kg(CO2).hm 2],显著(P 0.05)高于其他处理,比CK[3 646 kg(CO2).hm 2]增加103%,T2[2 735kg(CO2).hm 2]较CK减少25%(P 0.05)。紫云英水稻轮作方式增加了太湖地区水稻生长季的温室效应。     

水氮用量对设施栽培蔬菜地土壤氨挥发损失的影响

【目的】针对我国设施蔬菜生产中存在的水肥过量施用问题,研究不同水氮条件下黄瓜-番茄种植体系内的土壤氨挥发特征,探讨影响设施菜地土壤氨挥发的重要因子,为降低氮肥的氨挥发损失、 建立合理的灌溉和施肥制度提供参考。【方法】以华北平原设施黄瓜-番茄轮作菜地为研究对象,设常规灌溉(W1)和减量灌溉(W2)2个灌溉水平,每种灌溉水平下设不施氮(N0)、 减量施氮(N1)和常规施氮(N2)3个氮水平,共6个处理组合(W1N0、 W1N1、 W1N2、 W2N0、 W2N1、 W2N2)。采用通气法监测不同水氮条件下黄瓜-番茄轮作体系内的土壤氨挥发动态,分析与土壤氨挥发相关的主要影响因子。【结果】设施黄瓜-番茄种植体系内表层(0—10 cm)土壤铵态氮受施肥的影响波动较大,与常规施氮(N2)相比,相同灌水条件下减量施氮(N1)处理的0—10 cm土层铵态氮浓度最高值降低了25.1%~30.3%(P 0.05)。减量施氮可显著降低土壤氨挥发速率。与常规施氮(N2)相比,减量施氮处理(N1)在黄瓜季和番茄季内的氨挥发速率均值分别降低了21.1%~22.8%(P0.05)和16.5%~17.9%(P0.05)。整个黄瓜-番茄轮作周期内,土壤氨挥发损失量和氮肥的氨挥发损失率分别为17.8~48.1 kg/hm2和1.23%~1.44%。与常规施氮(N2)相比,减量施氮处理(N1)的土壤氨挥发损失量及氮肥的氨挥发损失率分别降低了19.3%~20.0%(P0.05)和0.85~0.92个百分点。各处理土壤氨挥发速率与0—10 cm土壤铵态氮浓度呈显著或极显著正相关,说明0—10 cm土壤铵态氮浓度是土壤氨挥发的重要驱动因子。与常规灌溉(W1)相比,减量灌溉(W2)条件下设施菜地土壤氨挥发速率及氨挥发损失量略有增加(P0.05)。适宜减少氮肥及灌溉量不仅能够维持较高的蔬菜产量,而且显著提高了灌溉水和氮肥的利用效率。其中减量施氮处理(N1)的氮肥农学效率比常规施氮(N2)提高了95.4%~146.4%; 减量灌溉(W2)的灌溉水农学效率比常规灌溉(W1)提高了27.7%~54.0%。【结论】通过合理的节水减氮措施可达到抑制氮肥氨挥发损失、 增加产量以及提高水氮利用效率的目的。在供试条件下,节水30%左右、 减施氮量25%的水氮组合(W2N1)具有较佳的经济效益与环境效应。     

尿素施用对稻田土壤甲烷产生、氧化及排放的影响

通过田间和培养试验研究了不施尿素(N0kg/hm2)和施用尿素(N300kg/hm2)对水稻生长期稻田土壤CH4产生潜力、氧化潜力和排放通量的影响。结果表明,水稻全生育期内,施用尿素降低土壤CH4产生潜力,并推迟其达到最大值的时间;尿素作为基肥和穗肥施用抑制土壤CH4氧化,而作为分蘖肥施用短暂地促进然后抑制土壤CH4氧化;施用尿素降低稻田CH4排放量。施用尿素通过同时影响CH4产生潜力和氧化潜力来影响稻田CH4排放。     

ALA提高丙酯草醚胁迫下油菜幼苗耐性研究

对油菜幼苗叶面喷施不同浓度的丙酯草醚(ZJ0273)和5-氨基乙酰丙酸(ALA),研究了ALA与丙酯草醚协同处理对苗期的油菜是否安全。结果表明,丙酯草醚随浓度从100~1000mg/L变化,逐渐抑制油菜幼苗生长,鲜重减少,净光合速率和叶绿素含量降低;过氧化物酶(POD)、超氧化物歧化酶(SOD)和抗坏血酸过氧化物酶(APX)活性下降,丙二醛(MDA)积累提高。而10~100mg/L的ALA则促进油菜幼苗生长,增加植株的鲜重、净光合速率并提高了叶绿素含量,POD、SOD和APX酶活性均比对照显著提高,MDA积累减少;500mg/L的ALA效果相反,表现为抑制作物生长。100mg/L ALA和不同浓度的丙酯草醚协同处理,油菜幼苗均比对照生长的更好,鲜重增加,净光合速率提高,抗氧化酶活性增加,MDA积累减少。因此,ALA可以提高油菜幼苗在除草剂丙酯草醚胁迫下的耐性。     

Effects of part and whole straw returning on soil carbon sequestration in C3–C4 rotation cropland

Long‐term no‐tillage management and crop residue amendments to soil were identified as an effective measure to increase soil organic carbon (SOC). The SOC content, SOC stock (SOCs), soil carbon sequestration rate (CSR), and carbon pool management index (CPMI) were measured. A stable isotopic approach was used to evaluate the contributions of wheat and maize residues to SOC at a long‐term experimental site. We hypothesized that under no‐tillage conditions, straw retention quantity would affect soil carbon sequestration differently in surface and deep soil, and the contribution of C3 and C4 crops to soil carbon sequestration would be different. This study involved four maize straw returning treatments, which included no maize straw returning (NT‐0), 0.5 m (from the soil surface) maize straw returning (NT‐0.5), 1 m maize straw returning (NT‐1), and whole maize straw returning (NT‐W). The results showed that in the 0–20 cm soil layer, the SOC content, SOCs, CSR and CPMI of the NT‐W were highest after 14 years of no‐tillage management, and there were obvious differences among the four treatments. However, the SOC, SOCs, and CSR of the NT‐0.5 and NT‐W were the highest and lowest in 20–100 cm, respectively. The value of δ¹³C showed an obviously vertical variability that ranged from –22.01‰ (NT‐1) in the 0–20 cm layer to –18.27‰ (NT‐0.5) in the 60–80 cm layer, with enriched δ¹³C in the 60–80 cm (NT‐0.5 and NT‐1) and 80–100 cm (NT‐0 and NT‐W) layers. The contributions of the wheat and maize‐derived SOC of the NT‐0.5, NT‐1 and NT‐W increased by 11.4, 29.5 and 56.3% and by 10.7, 15.1 and 40.1%, relative to those in the NT‐0 treatment in the 0–20 cm soil layer, respectively. In conclusion, there was no apparent difference in total SOC sequestration between the NT‐0.5, NT‐1, and NT‐W treatments in the 0–100 cm soil layer. The contribution of wheat‐derived SOC was higher than that of maize‐derived SOC.     

基于DNDC模型模拟江汉平原稻田不同种植模式条件下温室气体排放

稻田被认为是温室气体CH_4和N_2O的主要排放源之一。湖北省江汉平原地区水稻常年种植面积约8×105 hm2,占湖北省水稻种植面积的40%左右。研究江汉平原地区稻田温室气体排放特征,对于评估区域稻田温室气体排放以及稻田温室气体减排具有重要意义。目前,DNDC模型已被广泛应用于模拟和估算田间尺度的温室气体排放,DNDC模型与地理信息系统(Arc GIS)结合,可进行区域尺度的温室气体排放模拟与估算。本研究以湖北省典型稻作区江汉平原为研究区域,运用DNDC模型模拟和估算江汉平原稻田区域尺度的温室气体排放。设置大田定点观测试验,监测中稻-小麦(RW)、中稻-油菜(RR)、中稻-冬闲(RF)3种种植模式下稻田温室气体CH_4和N_2O的周年排放特征。通过田间观测值与DNDC模拟值的比较进行模型验证,并利用获取DNDC模型所需的气象、土壤、作物及田间管理等区域数据,模拟江汉平原稻田不同种植模式下温室气体CH_4和N_2O的排放量。田间试验表明,江汉平原稻田RW、RR和RF模型的CH_4排放通量为-2.80~39.78 mg·m-2·h-1、-1.74~42.51 mg·m-2·h-1和-1.57~55.64 mg·m-2·h-1,N_2O周年排放通量范围分别为0~1.90 mg·m-2·h-1、0~1.76mg·m-2·h-1和0~1.49 mg·m-2·h-1;CH_4排放量RW和RR模式显著高于RF模式,N_2O排放量为RF显著低于RW和RR模式。模型验证结果表明,不同种植模式温室气体排放实测值与模拟值比较的决定系数(R2)为0.85~0.98,相对误差绝对值(RAE)为8.29%~16.42%。根据DNDC模型模拟和估算的结果,江汉平原区域稻田CH_4周年的排放量为0.292 9 Tg C,N_2O周年的排放量为0.009 2 Tg N,不同种植模式稻田CH_4排放量表现为RWRRRF,N_2O排放量表现为RWRFRR,增温潜势(GWP)表现为RWRRRF。不同地区稻田CH_4排放量表现为监利县荆门市公安县天门市仙桃市洪湖市松滋市汉川市潜江市石首市荆州市江陵县赤壁市嘉鱼县,N_2O排放量表现为监利县荆门市公安县洪湖市仙桃市天门市汉川市潜江市松滋市荆州市江陵县赤壁市石首市嘉鱼县。本研究结果表明DNDC模型能较好地应用于模拟江汉平原稻田温室气体排放,RR和RF模式相比RW模式可有效减少温室气体CH_4和N_2O的排放。