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71.
K. ShankarganeshSuresh Walia Swaran Dhingra B. SubrahmanyamS. Ramesh Babu 《Pesticide biochemistry and physiology》2012,102(1):86-90
Resistance in Spodoptera litura (Fabricius) has been attributed to enhanced detoxification of insecticides by increased levels of esterases, oxidases and/or glutathione S-transferases. Enzyme inhibiting insecticide synergists can be employed to counter increased levels of such enzymes in S. litura. Dihydrodillapiole induced synergism of pyrethroid toxicity was examined in the laboratory-reared third instar larval population of S. litura collected in Delhi (susceptible), and Guntur (resistant) region of Andhra Pradesh, India. The Guntur population was found to be 7.04 and 10.19 times resistant to cypermethrin and lambdacyhalothrin, respectively. The activity of cypermethrin, lambdacyhalothrin and profenophos against susceptible and resistance populations of S. litura, was gradually increased when used along with a plant-derived insecticide synergist dihydrodillapiole. The α-naphthyl acetate hydrolysable esterase activity in Delhi population was less as compared to the Guntur population. Resistance associated esterases in Delhi population were inhibited by pre-treatment with dihydrodillapiole. The esterase level in insect was instantly reduced initially, sustained for about 3 h and equilibrated at 4 h post treatment. The esterase activity of Guntur population was increased to 1.28 μmoles/mg/min at 2 h post treatment and subsequently reduced to lower than 0.70 μmoles at 4-12 h post treatment. The variation in esterase activity is suggestive of its homeostatic regulation in test populations. Dihydrodillapiole thus caused significant reduction of resistance in S. litura to cypermethrin, lambda cyhalothrin and profenophos. 相似文献
72.
The tarnished plant bug (TPB) has increasingly become an economically important pest of cotton. Heavy dependence on insecticides, particularly organophosphates and pyrethroids, for TPB control facilitated resistance development to multiple classes of insecticides. To better understand resistance and explore ways to monitor resistance in field populations, this study examined acephate susceptibility and the activities of two major detoxification enzymes in nine field populations collected in the Delta region of Mississippi and Arkansas in 2010. Two Arkansas populations from Reed and Backgate had 3.5- and 4.3-fold resistance to acephate, as compared to a susceptible laboratory strain. Extensive planting of cotton and heavy chemical sprays is a major driving force for resistance development to acephate in Mid-south cotton growing areas. Reduced susceptibility to acephate was highly correlated with elevated esterase activities. The acephate-resistant populations from Backgate, Lula, and Reed consistently had higher (up to 5.3-fold) esterase activities than susceptible populations. Regression analysis of LC50s with kinetic esterase activities revealed a significant polynomial quadratic relationship with R2 up to 0.89. Glutathione S-transferase (GST) also had elevated activity in most populations, but the variations of GST activities were not significantly correlated with changes of acephate susceptibility. Finally, examination of esterase and GST inhibitors indicated that suppression rates (up to 70%) by two esterase inhibitors in 2010 were slightly lower than those detected in 2006, and ethacrynic acid (EA) inhibited GST effectively in both years. Two other GST inhibitors (sulfobromophthalein and diethyl maleate) displayed significantly lower suppression rates in 2010 than those detected in 2006, suggesting a potential genetic shift in pest populations and a necessity of continued monitoring for insecticide resistance with both bioassay and biochemical approaches. Results indicated that using major detoxification enzyme activities for resistance monitoring may provide insight into acephate resistance in field populations of TPB. 相似文献
73.
[目的]阐明Cd2+对花生(Arachis hypogaea L.)种子萌发的作用以及花生萌发过程中超氧阴离子(O2-.)含量的变化与酯酶同工酶、淀粉同工酶的关系。[方法]分别采用组织化学定位和电泳技术。[结果]10、30μmol/L Cd2+处理对花生胚根和下胚轴有显著的促进生长作用,使花生子叶中酯酶同工酶和淀粉同工酶活性增强,子叶以及胚根O2-.含量提高。[结论]酯酶同工酶和淀粉同工酶活性的提高与Cd2+诱导的超氧阴离子的提高相关。 相似文献
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77.
少果悬铃木遗传分析初步 总被引:5,自引:0,他引:5
通过对合肥等地选育出的一球悬铃木少果植株和正常结果植株的体细胞染色体组型分析和过氧化物酶同工酶酶谱分析,找出了两者遗传上的差异。 相似文献
78.
中国赖草(Leymus chinensis)为中国特有,用它与普通小麦(T. aestivum)杂交并经连续选育已获得小 - 赖麦(Triticum-Leymus)新种质7个品系,主要特点是高蛋白质、高赖氨酸含量和抗条锈、叶锈及白粉病。细胞学研究结果:F#-2时在1个完整细胞内只有1,2,3,4,6……等少数染色体,还有大环、长链、桥、落后染色体等异常现象。1987年观察了大量花粉母细胞(13892个)及根尖细胞(435个)染色体,从中筛选出7个品系,其2n=43 - 49者占75.63%,其中n = 22Ⅱ和2n = 49各占90%以上者各有2个品系。叶片酯酶同工酶分析结果表明杂种品系具有双亲互补酶带。 相似文献
79.
侧重分析了安徽省杂交西瓜在制种和生产上面积较大6个组合如新澄、新红宝、郑杂五号、金钟冠龙、皖杂一号和聚宝一号及其亲本种子幼苗体内的酯酶和过氧化物同工酶的组成情况和遗传特性。结果表明:1)6个组合中,除金钟冠龙外,其他5个组合的父本间和母本间酯酶酶谱表现相似;但每个组合父、母本间均存在差异。2)6个组合的F1代杂交种酯酶酶谱表现为母本不完全显性。3)除郑杂五号外,其他5个组合的F1代杂交种过氧化物酶谱和其父、母本间表现相似。另讨论了用酯酶同工酶筛选亲本组配杂交种和预测杂种优势的可行性。 相似文献
80.
黄颡鱼雌、雄个体血清蛋白、酯酶同工酶及血红蛋白的电泳分析 总被引:6,自引:0,他引:6
采用聚丙烯酰胺梯度凝胶垂直板(PAGE)电泳,对黄颡鱼雌、雄个体血清蛋白、酯酶同工酶(EST同工酶)及血红蛋白(Hb)进行了分析。结果表明:黄颡鱼雌、雄个体的血清蛋白存在明显差异,表现在B1区雄性个体比雌性个体多1条MI带,B2区雌性个体比雄性个体多1条MA带,少2条MⅡ带,B3区雄性个体比雌性个体多1条MA带。另外在B2区雌、雄个体间MA带和MI带排列次序也有差异。血清EST雌、雄个体也有明显的差异,雄性黄颡鱼血清EST的酶谱出现了3条酶带无中强带,而雌性黄颡鱼EST的酶谱出现了7条酶带,有4条中强带。雌、雄个体胁只能分离出1条谱带,并且雌、雄之间基本没有差异。 相似文献