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[目的]了解奶水牛乳汁体细胞计数(SCC)与抗氧化指标之间的相关性,为乳汁质量监控和奶水牛健康养殖提供科学依据.[方法]随机选取南宁某牛场奶水牛139头,同步采集乳样与血样,乳汁SCC采用FOSS体细胞仪测定,血浆和乳清的总抗氧化能力(TAC)采用FRAP法(血浆铁还原力法)测定,丙二醛(MDA)含量采用TBA法(硫酸巴比妥法)测定.[结果]被检的139头奶水牛中,乳汁SCC≥50.00万/mL(隐性乳房炎诊断标准)的样本占被检总数的12.23%,乳汁SCC<50.00万/mL的样本占被检总数的87.77%;奶水牛血浆和乳清的TAC分别为2.44±1.04和3.78±1.68 U/mL,MDA含量分别为2.07±0.48和1.55±0.52 nmol/mL,其差异均达极显著水平(P<0.01).乳清TAC随乳汁SCC的升高而呈下降趋势,二者间呈显著负相关(r=-0.168,P<0.05);血浆MDA与乳清MDA间呈显著正相关(r=0.189,P<0.05),而血浆MDA与血浆TAC(r=0.391)、乳清MDA与血浆TAC(r=0.315)间呈极显著正相关(P<0.01).[结论]乳清TAC可作为检测奶水牛乳房健康及其乳汁质量的备选指标. 相似文献
85.
腹泻是以排便次数增多、便稀溏为特征的一类疾病,根据不同特点又可分为泄泻、痢疾、便血三大类,每一类叉分为不同的证型。以临床病例为例,阐述腹泻的辨证方法、有效良方的选择及加减应用,既有同病同治、异病异治的常规方法,也有同病异治、异病同治的变化方法。 相似文献
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Salmonid sperm pre-incubated at extracellular pH (pHe) values less than about 7.4 do not become motile upon water activation whereas sperm maintained above about pH 8.0 demonstrate maximal motility upon activation. The basis for this permissive effect of elevated pHe on sperm motility is not known. Since it is conceivable that the pH sensitivity of dyneinATPase (the molecular motor that drives flagellar movement) could be the basis of, or contribute to this pH dependency, the pH sensitivity of this enzymatic activity was evaluated in membrane-permeabilized axonemes (isolated flagella) ofsteelhead sperm. DyneinATPase activity was found to be sensitive to pH. This activity in permeabilized axonemes was about 3.5-fold higher at pH 7.6 compared to 7.0. To determine whether the pH sensitivity ofATP regeneration might affect the interpretation of the effect of pH on dyneinATPase activity, the pH sensitivities of creatine kinase and adenylate kinase were established. The rates ofATP generation by these enzymes were insensitive to pH between 6.5 and 8.0. The results of these studies are consistent with the hypothesis that prior maintenance at pHe, in part, controls the potential for sperm motility upon water activation via an influence on dyneinATPase activity. However, the potential for motility ofsteelhead sperm is particularly sensitive to prior maintenance at pHe values between about 7.4 and 8.0 whereas the dyneinATPase activity of permeabilized axonemes was particularly sensitive to pH values between 7.0 and 7.6. Phosphorus NMR spectroscopy was used to determine that sperm intracellular pH (pHi) increased with increasing pHe between 7.0 and 8.5 and pHi was, on average 0.4–0.5 pH units lower than pHe. Therefore the pHe sensitivity of the potential for motility appears to correspond to the pHi sensitivity of dyneinATPase activity. The data indicate that pHi is directly related to pHe and that prior incubation at pHe may, in part, control the sperm's potential for motility upon water activation via an influence on dyneinATPase activity. 相似文献
88.
Cultured red porgy Pagrus pagrus (L.) males (n=6) were sampled every 2 weeks for milt, in order to monitor changes in sperm quality parameters during a whole spawning period. On 11 January 2001, 60% of the fish were spermiating, increasing to 100% in mid‐February and dropping to 30% by mid‐April. Sperm density showed a slight increasing trend, with mean values ranging between 8.6 and 23.7×109 spermatozoa mL?1. Sperm motility percentage exhibited a significant improvement during the spawning season (analysis of variance (anova ) P=0.0001). The duration of forward motility for the major part of the monitoring period ranged between 2 and 4 min. Red porgy spermatozoa maintained their viability for many days after whole storage of milt at 4°C. During the monitoring period there were significant changes in the mean duration of sperm survival after cold storage, ranging from 5 to 12 days. The total volume of expressible milt was maximal on 28 March, increasing from a mean value of 1.7 mL to 5.3 mL kg?1. Milt production of captive‐reared red porgy does not appear to be limiting, when compared with the volume of expressible milt produced by other cultured marine fishes. 相似文献
89.
Flocerfida Pagador Aquino Krystel Grace Vergara Lerma Cajuigan Ocampo Eufrocina dela Pena Atabay 《农业科学与技术》2014,(2):169-176
Due to the small volume of goat semen ejaculate, just a few doses of goat semen were produced when the sperm concentration is 100 × 10^/mL. The study was aimed to determine the viability of extended goat semen at refrigerated condition at 5 ℃ using varying sperm concentrations and evaluated if sperm concentration lower than 100 × 10^6/mL would affect the motility, viability and sperm morphology at refrigerated condition. Using an artificial vagina, ejaculated goat semen was collected from goat semen donor aged 1.5 year. Physical evaluation of the collected semen showed an average volume of 0.54 mL, mean pH of 6.8 and a milky white color with thick consistency indicative of high concentration. Fresh goat semen had an initial average of 76% with an average initial sperm concentration of 128 × 10^/mL. The semen was divided into four treatments: sperm concentration of 100× 10^/mL, 75 × 10^/mL, 50 × 10^/mL and 25 × 10^/mL, and were stored at 5 ℃ for a period of 10 d. The semen evaluation was performed for each of the four treatments every other day. Results showed that the sperm concentration of spermatozoa affected the duration of storage based on the sperm motility percentage, viability and morphology of spermatozoa. The extended goat semen with sperm concentration of 25 and 50 million sperm/mL is optimal for storage within 6 d that gave satisfactory percentage motile, viable and morphologically normal spermatozoa. 相似文献
90.
为研究小型震损水库溃坝风险,对水库的溃坝路径、溃坝概率、溃坝后果进行分析,建立风险评价模型,最终确定其风险指数。在简单介绍此模型的基础上,通过实例说明其在水库风险分析中的应用,确定模型的实用性及便捷性。 相似文献