几种鸡胚原代细胞的制备方法

在病毒分离、繁殖，中和试验及药物毒理试验中，细胞培养是经常被选用的技术。本文描述通过选择不同日龄的鸡胚，分别对皮肤上皮细胞、肝细胞和肾细胞进行了组织培养，确定了生长所需的培养液要求。认为１２日龄鸡胚适于皮肤上皮细胞的制备，１７～２０日龄鸡胚适合于肝细胞、肾细胞的制备，并确定了细胞生长的ｐＨ条件。     

日粮营养对动物脂肪组织基因表达的影响

脂肪组织是一个强大的分泌器官,它能分泌许多与能量、脂肪代谢相关的酶、激素等.调控能量代谢和脂肪组织生长.本文综述了日粮对动物脂肪组织中基因表达作用的研究状况.并阐述了营养成分对这些基因上下游表达的作用机制.     

Metal accumulation in House Sparrow (Passer domesticus) from Thohoyandou,Limpopo province,South Africa

Studies on metal pollution (cadmium, cobalt, chromium, copper, manganese and nickel) in South African terrestrial environments are severely lacking. Due to being relatively unaffected by industrialisation, the Thohoyandou region may provide data on natural levels of metals for use as baseline data. The House Sparrow (Passer domesticus) was chosen as a bio-indicator of metal pollution due to its abundance, non-migratory lifestyle and close association with humans. The aims were to determine the viability of using feathers as a non-lethal bio-indicator tissue compared with muscle. Plume feathers, flight feathers and muscle tissue were analysed using ICP-OES techniques. Analyses of tissue metal concentrations identified the following trend: plume feather > flight feather > muscle tissue. Within the Thohoyandou region, Magondi, which was affected by anthropogenic activities at the time of sampling, had significantly higher concentrations (p ≤ 0.05) for each of the metals, indicating potential metal contamination from various sources. Alternatively, Makonde had the lowest metal concentrations and may be an important reference site for future comparative studies. The results showed that the House Sparrow can be used as a bio-indicator organism in South African terrestrial environments. The plume feathers indicate that they are a good non-lethal tissue for determining metal pollution levels on a temporal scale, indicating recent metal exposure.     

Electrocardiographic,echocardiographic, and left atrial strain imaging features of a dog with atrial flutter and third-degree atrioventricular block

A 14-year-old American Staffordshire terrier was presented for episodes of exercise-induced syncope. At admission, atrial flutter coupled to third-degree atrioventricular block was diagnosed electrocardiographically. On the second day of hospitalization, surface electrocardiogram revealed spontaneous conversion to sinus rhythm with persistence of atrioventricular block. Complete transthoracic echocardiograms were performed after each electrocardiographic examination. The combined use of conventional echocardiography with tissue Doppler imaging-based modalities allowed to investigate the atrial electromechanical correlation and function during typical atrial flutter and after its resolution.     

MAGNETIC RESONANCE IMAGING AND PATHOLOGIC FINDINGS ASSOCIATED WITH NECROTIZING ENCEPHALITIS IN TWO YORKSHIRE TERRIERS

Two young adult Yorkshire terriers had neurologic signs consistent with forebrain and brainstem involvement or forebrain involvement alone. On magnetic resonance imaging studies there were asymmetric bilateral lesions mainly in the cerebral cortex, and in the diencephalon. These areas were hyperintense on T2-weighted and FLAIR images, but hypointense or isointense on T1-weighted images. Lesions had a varying degree of contrast enhancement. Areas which were isointense on T1-weighted images had no contrast enhancement or only foci of contrast enhancement. Lesions with hypointensity in T1-weighted images had no enhancement or more frequently ring-like enhancement around the lesion. Necrotizing encephalitis was confirmed pathohistologically in both dogs. The degree of contrast enhancement appeared to be related to the degree of lymphohistiocytic inflammation on histologic examination.     

PET/CT today and tomorrow in veterinary cancer diagnosis and monitoring: fundamentals,early results and future perspectives

Functional imaging using positron emission tomography (PET) plays an important role in the diagnosis, staging, image‐guided treatment planning and monitoring of malignant diseases. PET imaging complements conventional anatomical imaging such as computed tomography (CT) and magnetic resonance imaging (MRI). The strength of CT scanning lies in its high spatial resolution, allowing for anatomical characterization of disease. PET imaging, however, moves beyond anatomy and characterizes tissue based on functions such as metabolic rate. Combined PET/CT scanners were introduced commercially in 2001 and a number of technological advancements have since occurred. Radiolabelled tracers such as ¹⁸F‐fluorodeoxyglucose (FDG) and ¹⁸F‐fluorothymidine (FLT) allow visualization of various metabolic processes within cancer cells. Many studies in human oncology evaluating the utility of PET/CT have demonstrated clinical benefits. Few veterinary studies have been performed, but initial studies show promise for improved detection of malignancy, more thorough staging of canine cancer and determination of early response and disease recrudescence.     

花椰菜尾菜发酵饲料对保育猪生长性能、血清生化指标、小肠组织形态及经济效益的影响

为研究花椰菜尾菜发酵饲料对保育猪生长性能、血清生化指标、小肠组织形态及经济效益的影响.选取42头体重相近(11.40±1.36)kg、体况良好的40日龄保育猪,随机分为两组,每组3个重复,每个重复7头.对照组饲喂基础饲粮,试验组饲喂由80％基础饲粮和20％花椰菜尾菜发酵饲料组成的试验饲粮.试验共30 d,其中预试期8 ...     

几种匍匐翦股颖品种植株再生的研究

以匍匐翦股颖Agrostis stolonifera潘娜1号、海滨和普特的成熟种子为外植体,进行愈伤组织诱导、分化及生根研究。结果表明：在MS培养基上,以不同浓度2,4 D诱导潘娜1号、海滨和普特的愈伤组织时,普特的愈伤组织诱导率均高于潘娜1号和海滨。2 mg/L 2,4 D+0.1 mg/L 6 BA为潘娜1号和海滨诱导愈伤组织的最适激素组合,3 mg/L 2,4 D+0.1 mg/L 6 BA为普特愈伤组织诱导的最适激素组合。诱导率分别为45.2%、76.5%和87.0%;MSO(MS培养基的大量、微量元素和铁盐+B5培养基的有机元素)为最适合的分化培养基,分化率分别为50.0%、59.0%和57.6%。1/2 MS +1.0 mg/L IBA为生根培养基。     

USE OF COMPUTED TOMOGRAPHY AND RADIOLABELED LEUKOCYTES IN A CAT WITH PANCREATITIS

The normal feline pancreas has been evaluated using radiolabeled leukocytes (99mTc-HMPAO) and computed tomography. The purpose of this report is to describe a clinical case where both modalities were utilized to assess the inflamed feline pancreas. A nine year old female cat presented with anorexia, depression and some vomiting. Blood values were unremarkable. Radiographs and ultrasound were suggestive of pancreatitis. The cat's leukocytes were separated and labeled according to an established protocol. Whole body images were acquired immediately, at 5 and 30 min, and at 1, 2, 4, and 17 hours post injection. Approximately 48 h later, the animal was anesthetized and computed tomography of the abdomen was preformed both pre and post contrast. Surgical biopsies were taken. The distribution of the WBCs was similar to that documented in normal animals, however, at 2 h there was faint uptake seen in the region of the pancreas. This uptake became more intense at 4 h and persisted at 17 h. Computed tomography showed irregular margination of the pancreas, it was larger than normal and inhomogeneous. Contrast enhancement was inhomogeneous and its peak enhancement was not reached until 10 min post injection; normal feline pancreas enhances homogeneously and peaks immediately. Histopathology confirmed pancreatitis with lymphocytic, plasmacytic, neutrophilic and eosinophilic inflammation and fibrosis. Radiolabeled leukocytes can be used to document pancreatic inflammation and this is best seen 4 h after injection. Computed tomography allows superior visualization of the pancreas. Both the appearance and contrast enhancement pattern of the inflamed pancreas differ from normal.     

新疆药桑组培育苗的方法与培养基筛选试验研究

新疆药桑的组培育苗需要经过初代培养、继代培养、生根培养、驯化移栽几个阶段。经试验比较筛选出了适合不同阶段生根发苗的培养基配方,并阐述了新疆药桑组培快繁的方法和步骤。