排序方式: 共有85条查询结果,搜索用时 15 毫秒
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为了给中国家驴遗传资源的评价、保护和利用提供一些分子生物学基础,本研究通过自行设计引物,采用PCR-SSCP技术对中国家驴线粒体DNA( Mitochondrial DNA,mtDNA)细胞色素b(Cytochrome b,Cytb)基因308bp片段序列的变异进行检测来探讨其遗传多样性和母系起源.发现了3种基因型,结合GenBank已公布的数据进行分析发现在中国家驴品种中共存在15个突变位点,17个单倍型,通过计算各群体的遗传多样性指数发现中国家驴的遗传多样性比较丰富.另外,还通过与两种非洲野驴的Cytb序列进行比对和网络聚类分析方法对所有家驴的样品进行了母系起源的研究,证明家驴起源于非洲的索马里驴和努比亚驴. 相似文献
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El-Sayed A.A. El-Shafaey Gamal I.A. KarroufAdel E.I. Zaghloul BVSc MVSc PhD 《Journal of Equine Veterinary Science》2012
Little is known about using magnetic resonance imaging (MRI) in evaluating the healing properties of superficial digital flexor tenorrhaphy in equines. The present study was designed to carry out in vivo comparative evaluation of normal and repaired superficial digital flexor tendon (SDFT) in donkeys, based on MRI, ultrasonography, clinical and histopathological features. Eighteen clinically healthy donkeys were assigned for three treatment trials (n = 6) using three bioscaffold materials (bovine pericardium xenograft, tendon allograft, and allograft shielding by bovine pericardium), in addition to six healthy donkeys to serve as control group. Clinical signs for each animal were scored, and the sum of all clinical indexes was calculated. MRI and ultrasonographic examinations of each donkey were carried out at 45 and 90 days postoperatively in the three treated groups, and were compared with those in the control group. Both clinical and imaging characteristics were compared and statistically analyzed at each time point. The echogenicity (P < .0039), cross-sectional area (P < .0490), and fiber alignment (P < .0077) were different at 45 and 90 days postoperatively in the shielding group than in the other two groups, with no difference in the histological features in comparison with the control group, based on the median and range analysis at 90 days postoperatively. In conclusion, MRI and ultrasonography initiate a new paradigm in monitoring the healing progression and case prognosis of SDFT tenorrhaphy with the bioscaffold augmentation devices, either xenogenic or allogenic, which provided a good alternative technique and an accelerated SDFT healing with minimal adhesions in donkeys. 相似文献
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M. Bugno J. Klukowka-Rötzler E. Sota W. Witarski V. Gerber & T. Leeb 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2007,124(3):172-174
The physical localization of the epidermal growth factor receptor (EGFR) gene was performed on donkey chromosomes. Bacterial artificial chromosome DNA containing the equine EGFR gene was used to map this gene by fluorescent in situ hybridization on donkey metaphase chromosomes. The gene was mapped on donkey 1q21.1 region. 相似文献
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The Effects of Verapamil Hydrochloride on Electrocardiographic (ECG) Parameters of Domestic Donkey (Equus asinus) 总被引:1,自引:0,他引:1
Gholam Ali Kojouri DVM PhD Ali Rezakhani DVM PhD Ehsan Torki DVM 《Journal of Equine Veterinary Science》2007,27(11):499-503
Verapamil hydrochloride, a calcium channel blocker, has been used in domestic animals. Its effects on electrical activities of the heart of donkeys were studied in this preliminary research work. This experiment was carried out on 10 healthy donkeys ranging in age from 4 to 7 years and mean body weight of 155 ± 18.6 kg. The electrocardiogram (ECG) was recorded on a base apex lead before (for 3 days), during, and after verapamil injection (10, 20, 30, 40, 50, and 60 minutes). Verapamil hydrochloride (0.3 mg/kg body weight) was given via catheterizaion as an intravenous bolus over 2 minutes. Heart rate, rhythm, ECG morphology, amplitude, durations, and intervals were measured and taken into consideration and values were compared. Results showed that heart rate was significantly increased at the time of injection from 55 ± 10.7 before injection to 77.61 ± 12.6 beats per minute after injection (P < .05). Duration of P and T waves; P-R interval, and also P-R segment were increased (P < .05). However, duration of QRS complex, P-P and R-R intervals, and S-T segment were significantly decreased after verapamil injection (P < .05). Sinus arrest (2 cases), bradycardia (1 case), and wandering pacemaker (1 case) were recorded before treatment and verapamil injection did not affect these arrhythmias, except in one case in which sinus arrest disappeared. However, after injection of verapamil, four types of arrhythmias (wandering pacemaker [WPM], sinus tachycardia, 1st degree AVB, and 2nd degree AVB) were recognized. These arrhythmias (with the exception of tachycardia) can be related to the increase of vagal tone activity that might be attributed to the negative effect of verapamil on SA and atrioventricular block [AV] nodes. 相似文献
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对68头乐都县本地驴的9项血液生理指标进行测定,结果:RBC5.43±0.88×10 ̄(12)/L;HB132.4±12.5g/L;PCV0.38±0.04L/L;MCH24.98±4.15pg;MCV71.01±11.48fl;MCHC352.36±28.89g/L;WBC11.21±2.56×10 ̄9/L;WBC-DC中,嗜酸性白细胞14.99±6.90%、嗜碱性白细胞0.04±0.21%、嗜中性白细胞40.87±9.86%(内杆状核5.16±3.92%、分叶核35.66±9.71%)、淋巴细胞42.76±10.81%、单核细胞1.24±1.76%;ESR中,15min11.64±15.64mm、30min23.76±19.57mm,45min31.88±19.22mm,60min35.38±18.87mm。 相似文献
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青海本地驴血清白蛋白及其多态性研究 总被引:1,自引:0,他引:1
采用折射计法和聚丙烯酰胺凝胶电泳法对87匹青海本地驴血清白蛋白及其多态性进行了研究。结果表明:①青海本地驴血清白蛋白含量为34.29g/L,占血清总蛋白的46.65%;②前白蛋白位点呈单态;③白蛋白位点有ALBCC和ALBCD两种表型,其ALB ̄C和ALB ̄D等位基因频率分别为0.8563和0.1437;④后白蛋白位点有PoAAA,PoAAB和PoABB三种表型,其PoA ̄A和PoA ̄B等位基因频率分别为0.1163和0.8837。 相似文献
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Ethel V. Taylor DVM Courtney L. Baetge DVM Nora S. Matthews DVM Tex S. Taylor DVM Kerry S. Barling DVM PhD 《Journal of Equine Veterinary Science》2008,28(5):295-300
The purpose of this study was to determine a satisfactory combination of guaifenesin, ketamine, and xylazine (GKX) that would produce safe and satisfactory total intravenous anesthesia in donkeys for use under field conditions. Donkeys require higher amounts of ketamine in GKX to achieve satisfactory anesthetic levels without producing excessive depression with guaifenesin. Five adult standard donkeys (average weight, 264 kg) were anesthetized with 1.5 mg/mL ketamine, 0.5 mg/mL xylazine, 50 mg/mL guaifenesin (GKX-1); 2.0 mg/mL ketamine, 0.5 mg/mL xylazine, 50 mg/mL guaifenesin (GKX-2); or 2.0 mg/mL ketamine, 0.75 mg/mL xylazine, 50 mg/mL guaifenesin (GKX-3). For the first trial, two donkeys received GKX-1, two received GKX-2, and one received GKX-3. One donkey received GKX-1, one received GKX-2, and three received GKX-3 for the second trial. In the final trial, two received GKX-1, two received GKX-2, and one received GKX-3. Donkeys were sedated with xylazine (1.1 mg/kg body weight) intravenously, and anesthesia was induced using intravenous GKX-1, GKX-2, or GKX-3. Anesthesia was maintained for 45 minutes; temperature, respiration rate, heart rate, hemoglobin saturation, partial pressure of arterial oxygen (PaO2), partial pressure of carbon dioxide in arterial gas (PaCO2), and pH were measured. There was no significant difference between combinations for temperature, respiration rate, heart rate, hemoglobin saturation, PaCO2, or pH. At 30 and 45 minutes, GKX-3 produced significantly (P < .05) lower PaO2 values than GKX-1 and GKX-2. GKX-3 is not recommended for field use in donkeys because of respiratory depression (PaO2= 48.7 [±5.84] and 46.0 ± 3.11 mmHg at 30 and 45 minutes, respectively), whereas more voluntary movement was apparent with GKX-1. GKX-2 produced satisfactory anesthesia without significant respiratory depression in donkeys and should produce safe and effective anesthesia in donkeys under field conditions. 相似文献
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德州驴不同组织材料总DNA提取的有效方法 总被引:1,自引:0,他引:1
[目的]为了分析德州驴的种群结构和种间遗传相似性关系,以及预测选配和杂交配合等方面提供一定的科学依据,并为做好德州驴的保种育种工作提供一个新的思路。[方法]根据德州驴血液、肌肉和毛发3种样品的特点,分别采用相适合的方法提取基因组DNA,并对提取的基因组DNA进行检测。[结果]结果表明:这3种DNA抽提方法及相应改进的可靠性,得到的DNA纯度较高。[结论]该方法可应用于PCR扩增等后续分子生物学试验研究。 相似文献