廊坊部分地区肉鸡白痢的发病特点调查报告

本调查了肉鸡白痢的发病特点，结果表明，5-14日龄发病率最高，鸡群发病率为9.7%，死亡率为3.4%。结果表明：恩诺沙星对鸡白痢的治愈率为94.4%。     

Chicken egg yolk antibodies (IgY) as non-antibiotic production enhancers for use in swine production: a review

In recent years, the use of in-feed antibiotics for growth and disease prevention in livestock production has been under severe scrutiny. The use and misuse of in-feed antibiotics has led to problems with drug residues in animal products and increased bacterial resistance. Chicken egg yolk antibodies (IgY) have attracted considerable attention as an alternative to antibiotics to maintain swine health and performance. Oral administration of IgY possesses many advantages over mammalian IgG such as cost-effectiveness, convenience and high yield. This review presents an overview of the potential to use IgY immunotherapy for the prevention and treatment of swine diarrhea diseases and speculates on the future of IgY technology. Included are a review of the potential applications of IgY in the control of enteric infections of either bacterial or viral origin such as enterotoxigenic Escherichia coli, Salmonella spp., rotavirus, porcine transmissible gastroenteritis virus, and porcine epidemic diarrhea virus. Some potential obstacles to the adoption of IgY technology are also discussed.     

猪流行性腹泻病毒RT-PCR检测方法建立及初步应用研究

为建立一种特异、敏感的PEDV的检测方法。根据GenBank中公布的猪流行性腹泻病毒CV777株的M基因序列,设计了一对特异性引物,扩增长度为680 bp的片段。将PCR产物进行测序,与CV777株、SH5株M基因的同源性分别为99.2%和97.6%。试验表明：该方法具有较好的特异性和敏感性,并能检测到PEDV的RNA浓度下限为100 pg/μL。从福建省的3个地区共采集58份哺乳仔猪腹泻样品,并应用此方法进行检测,其阳性检出率为87.9%。     

日粮不同粗纤维水平对幼兔生长发育的影响研究

以120只40日龄的新西兰兔为试验材料,随机分为A、B、C和D共4组。在其它营养水平相同的情况下,研究粗纤维水平分别为10%、11%、12%和13%的4种日粮对幼兔日增重、料肉比、腹泻率、经济效益等指标的影响。结果表明,试验期平均日增重以A组最高(27.78±3.85)g,且随着粗纤维水平的增加而逐渐减少(P<0.05);A组和D组分别具有最低(3.81)和最高料肉比(4.26),差异达到显著水平(P<0.05);随着粗纤维水平的逐渐增加,腹泻率呈显著下降趋势,粗纤维水平为10%时腹泻率达到26.67%;经济效益分析发现,C组的产出投入比最高。     

两种教槽料对仔猪断奶前生长性能和腹泻发生率的影响

该试验主要是针对本场两种不同性状教槽料对哺乳仔猪生长性能及腹泻发生率进行对比分析试验,并评估其经济效益。试验选取了54头出生时发育良好,体况健康的仔猪,随机分成两个处理组,每个处理组3个重复,每个重复9头仔猪。分别饲喂两种教槽料：试验Ⅰ组饲喂康地5900,试验Ⅱ组饲喂安佑人工乳,试验期为20d。结果表明：康地5900和安佑人工乳进行比较,平均日增重差异显著（P〈0．5）,平均日采食量差异显著（P〈0．5）,料肉比差异显著（P〈0．5）。仔猪断奶前,饲喂康地5900可明显提高仔猪的生长性能和降低腹泻率。     

Tolerance of a rice-based oral rehydration solution given to normal calves

The objective of the study was to test the tolerance of a rice-based oral rehydration formula when fed to calves. Six healthy Holstein calves, 1 week of age, were fed the formula instead of milk replacer for 3 days. Pre- and posttreatment results of clinical examination and laboratory parameters were compared. Vital signs, attitude, appetite, clinical hydration status, urine specific gravity, and most routine serum biochemistry test results did not vary and remained within the normal range. Five of the 6 calves developed diarrhea when fed the rice-based formula, which was accompanied by a reduction in fecal pH and presence of reducing sugars in the feces. This effect was reversed when calves were returned to the milk replacer diet at the end of the study. Diarrhea was accompanied by increased water consumption, which allowed the calves to maintain normal hydration status. These results suggest that calves are unable to properly digest the rice-derived carbohydrate, and this type of formula is not recommended for oral rehydration of calves.     

Antimicrobial resistances,and molecular typing of Campylobacter jejuni isolates,separated from food-producing animals and diarrhea patients in Iran

The aims of this study were to regain new epidemiology information about frequency, drug resistance rates, and typing of Campylobacter jejuni (C. jejuni) isolates, obtained from some poultry and cattle farms, slaughterhouses, and people with diarrhea. In this regard, Minimal Inhibitory Concentration (MIC) of several antibiotics and the associated antibiotic resistance genes, including tetO, tetA, cmeB, and bla_OXA-61 were evaluated. The isolates were also typed, using the Fla-RFLP method. Generally, between 233 food animal samples, 80 (34.33%) C. jejuni were isolated. Moreover, 20 out of 74 (27%) human specimens suspected to infectious diarrhea were C. jejuni positive. High frequencies of resistance to tetracycline (100%), ciprofloxacin (95%), and nalidixic acid (86%), and low frequencies of resistance to florfenicol (0%), erythromycin (5%), and gentamicin (8%) were observed. Furthermore, in the tetracycline-resistant isolates, the existences of tetO, tetA, and cmeB were 86%, 23%, and 48%, respectively. There was a significant correlation between the cluster types obtained from Fla-RFLP method and antibiotic resistance pattern. The results suggested that the genomic link between Campylobacter spp. should be always evaluated in each country to provide an insight about the Campylobacter spp., spread in the region, in order to implement the health-controlling programs efficiently.     

青贮苹果渣作酸化物饲喂断奶仔猪试验

用2％、4％、6％青贮苹果渣(按风干物计)与1.5％柠檬酸(对照组)饲喂断奶仔猪,以探讨青贮苹果渣有机酸对仔猪的饲喂效果。选用64头35～40日龄仔猪,体重7～8kg,饲喂45d,结果表明:2％、4％、6％与对照组1～20d日增重依次为312g、322g、303g、324g;21～45d为603g、590g、582g、590g,全期平均日增重为473g、470g、458g、471g,饲料增重比为2.26、2.27、2.34、2.27。组间差异均不显著(P>0.05),仔猪严重腹泻率依次为4.8％、4.2％、3.8％、3.9％,差异不显著(P>0.05)。4％组与对照组血清中钙、磷、二氧化碳结合力、谷雨转氨酶、总蛋白含量非常接近。结论:青贮苹果渣饲喂断奶仔猪效果与1.5％的柠檬酸饲喂效果无差异。仔猪日粮中青贮苹果渣的适宜用量为4%左右。     

一例仔猪腹泻的病原学诊断

为了解某猪场仔猪腹泻发生的原因,对病死仔猪进行病原学诊断.结果：检测出PCV20RF2特异性片段,并分离鉴定到埃希氏大肠杆菌,未发现TGEV与PEDV感染,表明导致仔猪腹泻的原因是PCV2继发感染大肠杆菌所致.