Vascular ultrasonographic findings in canine patients with clinically diagnosed phlebitis

Peripheral vein phlebitis (inflammation) is a relatively frequent complication in dogs, however, published information on the ultrasonographic characteristics is currently lacking. This prospective, observational study describes the ultrasound (US) characteristics of normal canine cephalic veins, and veins with clinical phlebitis. Correlations among US findings and between US findings versus time that the intravenous catheter was in place were investigated. Safety of the US procedure was evaluated. Fifty patients were prospectively recruited for the study and 18 met the final inclusion criteria. Each patient underwent daily US examinations and was assessed for multiple criteria (vascular wall appearance, compressibility, spontaneity of flow, color fill, and presence/absence of filling defects, flow contour, direction, non‐pulsatility). Characteristics of normal canine cephalic veins were as follows: smooth and thin wall, complete compressibility, no flow disturbances, no filling defects, smooth flow contours, and unidirectional, non‐pulsatile flow with no turbulence. Characteristics of cephalic veins with clinical phlebitis were as follows: wall thickening (83%), decreased compressibility (55%), filling defects consistent with intraluminal thrombus (55%), vessel wall hyperechogenicity (44%), and abnormal color Doppler flow (39%). Significant correlations were found between Doppler filling defects and compressibility, Doppler filling defects and presumed thrombosis, and compressibility and presumed thrombosis (P = .001, P = .001, P = .000, respectively). No correlation was found between the US findings and time the intravenous catheter was in place. Findings indicated that duplex and compressibility US are feasible and safe methods for characterizing and monitoring cephalic veins in dogs with clinical phlebitis.     

北极狐出血性肠炎病原分离鉴定

从排血便为主要临床特征的濒死期育成北极狐肠内容物中分离到 ５ 株革兰氏阳性大杆菌,各株菌的 ３７℃ ８ 小时厌气肉肝汤纯培养上清液 ０２ｍ ｌ小鼠尾静脉注射,１２ 小时内 １００％ 死亡。生理生化鉴定证实分离菌为产气荚膜杆菌（ Ｂ．ａｅｒｏｇｅｎｅｓｃａｐｓｕｌａｔｕｓ）。血清定型结果表明,分离的 ５ 株菌均为 Ａ 型产气荚膜杆菌。以饲喂狐的变质鱼浸出液静脉注射小鼠引起死亡,并从该浸出液中也分离到 Ａ 型魏氏梭菌,证实该病的发生是饲料传播。     

Molecular cloning and expression analysis of pig CD81

CD81, also known as TAPA-1 (target of antiproliferative antibody 1), is a member of the tetraspanin family of proteins and a component of the B cell co-receptor complex. Several studies have shown that CD81 plays significant roles in a variety of immune responses, including activation of B cells and T cells. In this study, we cloned pig Cd81 cDNA using RT-PCR coupled with rapid amplification of cDNA ends (RACE)-PCR and determined the complete cDNA sequence of pig Cd81. Pig Cd81 cDNA contains an open reading frame (711 bp) encoding 236 amino acids. The identity of pig CD81 with those of human, cattle, rat, and mouse are 90.30%, 92.26%, 86.22%, and 86.22%, respectively. Alignment of the CD81 amino acid sequence with those of mammalian species showed that the large extracellular loop (LEL) is the most divergent, whereas other domains are largely conserved. Pig Cd81 mRNA was detected by RT-PCR in a broad range of tissues, including lymphoid tissues as well as nonlymphoid tissues, indicated variety of cellular functions of CD81 in most pig tissues. Flow cytometry analyses demonstrated that human CD81 antibody recognizes a pig CD81 on the cell surface. Further, immunohistochemistry analysis using human CD81 antibody on pig spleen was revealed that CD81 expression is widely diffused in spleen tissue. Future study will be focused on defining the functional role of CD81 during the course of pig infectious diseases.     

鸡传染性贫血病毒感染对雏鸡免疫系统的影响 Ⅰ.T、B淋巴细胞增殖反应的变化

1日龄雏鸡感染鸡传染性贫血病毒（CIAV）后,胸腺和脾脏T淋巴细胞增殖反应分别于14d和7～21d明显减弱（P相似文献   

我国西藏四种植物染色体数目观察

本文对西藏四种植物进行染色体观察，这四种植物的染色体数目均为国内外首次报道。     

Research Progress on Rapid Serological Assays for Diagnosis of Japanese Encephalitis B

Japanese encephalitis B (JE) is a zoonosis caused by Japanese encephalitis virus which invades the central nervous system,infected by mosquito.Various animals could be the sower and reservoir of JE including swine,horse,cattle and avian.JE could cause a major public health trouble and a horrible crisis of the farm all in Asia and the Western Pacific region.Therefore,it is necessary to diagnose it rapidly and accurately.This review article made a sketch of approaches for the rapid serological assays of JE,including traditional method,emerging technologies and specific improvements of the assay,so as to provide references for the study of rapid,simple and accurate JE diagnose methods.     

犬种布氏菌地方株的DNA中G Cmol%测定

首次利用热变性法对9株地方犬种布氏菌DNA的G C含量进行了测定,同时以2株国际标准株为对照。G C mol％值在56.6～58.4范围内,与文献报道的犬种布氏菌和我们所用的2株标准株所得的G C mol％值是一致的。各株菌经常规检定、噬菌体裂解试验、血清学检查所得的结果均符合于犬种布氏菌的特性。说明本试验测定的G C mol％值代表了犬种布氏菌的DNA分子结构比值。为我国地方菌种、特别是粗糙型布氏菌的检定提供了一个新的方法。此外,对传统的DNA提取法进行了改进,使之更便于本试验的要求。