马立克氏病毒CVI988的TK及gB基因启动子的扩增

根据GenBank公布的MDV GA株全基因组序列设计引物,以CVI 988/R ispens感染鸡胚成纤维细胞的基因组总DNA为模板,利用PCR的方法分别扩增了CVI 988/R ispens的TK及gB基因启动子基因,克隆入pGEM-T-easy载体并进行测序,结果表明CVI 988/R ispens的TK及gB基因启动子序列均存在TATA盒和CAAT盒等真核启动子所具有的保守序列,且与MDV GA株的相应基因序列完全一致,为进一步研究该启动子在MDV病毒载体中的表达调控提供了物质条件。     

表达NDV F48E9株F基因的MDV CVI988株转移质粒载体的构建

将新城疫病毒(NDV)F48E9株融合蛋白(F)基因1700bp克隆到真核表达载体pIRES中,构建成表达F基因的载体pIRESF,然后将包含F基因及其上游的内含子和下游的polyA的2900bpDNA片段再克隆入包含gB启动子的SK载体中,并使其克隆到gB启动子600bp的下游,最后将包含gB启动子和F基因表达盒3500bp克隆到包含MDVCVI988非必须片段US10的载体SK中。该研究为进一步在细胞中转染并获得表达F基因的MDVCVI988重组病毒奠定了基础。     

Patterns of tropical forest dynamics and human impacts: Views from above and below the canopy

Tropical forests are influenced by regional and global bio-climatic processes as well as local anthropogenic disturbances. Most studies have ignored the synergistic influence of bio-physical processes operating at large spatial scales and local human use on forest vegetation and fauna. Assessments of forest condition change using time-series of remotely sensed data need to be supported by measurements under the canopy. The Tadoba-Andhari Tiger Reserve (TATR) in India is a protected area that has a long history of human resource extraction and settlements. Like much of South Asia, it has undergone major shifts in rainfall in the last hundred years. We examine trends in forest greenness over two and half decades and assess spatial patterns in rates of change. We also analyze ground based measurements of human impacts on flora and fauna. Trends in forest canopy greenness show two distinct phases: a period of decline from 1980s to mid-90s, followed by a recovery. These trends are a function of initial greenness and are best explained by prevailing climatic regimes, feed-backs from human use, and park management practices and protection. Negative impacts to flora and fauna on the ground were, however, wide-spread during the recovery period and are influenced by proximity to nearest settlement as well as combined distance from all settlements. Remotely sensed data cannot effectively detect these processes under the canopy. There is an urgent need to incorporate monitoring of long-term bio-climatic processes and their interaction with short and long-term effects of human-use and disturbance arising from processes at local, regional and larger spatial scales around protected areas to effectively manage these reserves.     

基于虚拟仪器技术的食品物性检测系统的研究

采用虚拟仪器技术、机电一体化技术、单片机技术、传感器技术于一体,研制了食品物性参数的自动检测和分析系统。系统硬件由食品受力传感器、食品变形位移传感器、定时器、信号调理、数据采集、串口通讯、扩展LCD等模块电路构成;软件基于Lab Windows/CVI虚拟仪器开发平台,采用多线程技术实现了信号采集、动态曲线显示、试验数据的实时分析和处理。试验结果表明：该系统的检测精度高、检测功能完善及抗干扰能力强,具有理论研究意义和实用价值。