关于TQI、TQP、T.M平坦模、特殊环和模

本文借助左模范畴Ｒｍｏｄ的一个遗传扭论（Ｔ,Ｆ）和相应的Ｇａｂｒｉｅｌ拓扑Ｇ,讨论关于扭类的ＴＱＩ模、Ｔ．Ｍ平坦模和ＴＱＦ模。最后还特征化了扭半单模、扭半单环和扭正则环,推广了半单模、半单环和正则环。     

表达MDVgB基因重组痘苗病毒的构建及保护性研究

以脂质体转染技术构建了表达鸡马立克氏病毒(MDV)gB基因的重组痘苗病毒(RVV-gB), 表明该病毒在CV-1(猴肾细胞)细胞系内能稳定传代,经腹腔1×106 PFU·羽 -1 将重组病毒、HVT冻干苗及痘苗病毒分别按试验程序,免疫1日龄SPF鸡,并于15日龄以3×10 2PFU·羽-1MDV强毒株GA株攻毒,重组病毒和HVT冻干苗匀获得较高的保护, 结果证明了gB是MDV的宿主保护性抗原.此研究为CTL应答检测奠定了基础.     

犬脾脏转移因子的提取与鉴定

本试验用犬瘟热弱毒苗免疫的犬,提取了特异性犬脾转移因子。经理化性质测定,本品不含蛋白质,核糖含量为３０１．６ｕｇ／ｍＬ,含有１１种氨基酸。生物学特性测定表明,本品无菌、无热原、无毒性、无抗原性。兔注射特异性犬脾ＴＦ后,经酯酶染色法测定,Ｔ淋巴细胞比值由注射前的４０．４１％上升到注射后的６３．４５％。     

籼型水稻光、温敏核不育系配合力研究

选用 6个籼型光 (温 )敏核不育系 ,5个籼型父本 ,按 6× 5NCⅡ设计 ,共配 30个组合 ,对亲本一般配合力 (GCA)及杂种的特殊配合力 (SCA)进行了研究 ,结果如下 :(1)筛选了一批优良的两系亲本材料 ,不育系中籼型以X0 7S、3418S为好 ,父本中紫恢10 0、明恢 6 3、2 346配合力为好。 (2 )在本研究中播始历期和结实率SCA与GCA的关系是相对独立的 ,籼杂中株高、穗实粒 ,有一定的联系 ,高特殊配合力的组合 ,一般至少有一方亲本的一般配合力高。 (3)初步筛选了 3418S/2 346、3418S/明恢 6 3、X0 7S/2 346、X0 7S/紫恢 10 0等强优组合 ,在本试验中单株产量均显著超过汕优 6 3。文中对供试不育系和初步筛选的强优组合应用前景进行了讨论     

流式细胞术检测TcR Vα24/Vβ11双阳性NKT细胞及其在HCV感染者中的应用

目的研究丙肝病毒感染者外周血NKT细胞含量与HCV感染状态之间的相关性.方法应用流式细胞术检测TcR Vα24/Vβ11双阳性NKT细胞.结果抗-HCV阳性且HCVRNA也阳性的病例NKT细胞数量明显低于抗-HCV阳性而HCV RNA阴性的病例（P〈0.01）,明显低于正常对照组（P〈0.05）.结论由于NKT细胞数量减少或活化受阻,造成HCV的持续感染,不利于病毒的清除;另一方面,由于肝内有HCV的存在,NKT细胞大量向肝内趋化,导致外周血的数量相对减少.     

雷公藤和昆明山海棠形态变异的研究

对雷公藤种质资源进行调查,建立种质资源收集库,定期观察雷公藤表型性状,研究形态标记的遗传变异,结果表明,雷公藤与昆明山海棠的叶片大小、叶背白粉、花序大小等主要分类特征存在显著的差异,但其变异是连续的,而且地理分布也是连续的;昆明山海棠、雷公藤均能适应长江流域的气候条件,但不同种源生长情况及主要活性成分含量存在明显的差异.雷公藤与昆明山海棠现行分类问题值得商榷,两者应属同一种植物,雷公藤与昆明山海棠的遗传多样性预示着遗传改良具有巨大的潜力.     

厚皮甜瓜新品种‘京蜜8号’的选育

‘京蜜8号’是以‘B203’为母本、‘T24’为父本选育而成的杂交1代厚皮甜瓜新品种。该品种植株长势稳健,中晚熟,全生育期115～120 d,果实发育期46～50 d。果实椭圆形,果形指数1.21,果皮浅黄色,覆白色网纹,外形美观,果肉浅橙色,肉质松脆,口感清香,果肉厚度约3.9 cm,单果质量1.50 kg左右,667 m^2产量约2 600 kg。中心可溶性固形物含量(w,后同)15.5%～16.5%,边部11.5%。不脱蒂,极耐贮运。适宜北京、河北、河南、内蒙古、浙江、山东、陕西等地区设施栽培,2016年7月通过北京市种子管理站鉴定。     

Comparative analysis of genetic effects of wheat‐Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL

Wheat‐Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL, carriers of Pm21 and PmV, respectively, confer high resistance to wheat powdery mildew. For better understanding of the difference in genetic effect between them, a RIL population was constructed based on the cross between “Yangmai 18” carrying T6V#2S·6AL and “Yangmai 22” carrying T6V#4S·6DL. Analysis of distribution of the translocations showed that T6V#2S·6AL is much more transmittable than T6V#4S·6DL. By comparing their effects on main agronomic traits, we firstly found that T6V#2S·6AL contributes greatly to top spikelet fecundity, but causes a decrease of 6.7%–10.5% of spike number. No stable effects of T6V#4S·6DL on agronomic traits were found, except for positive effect on plant height. Excitingly, a new recombinant, T6V#4S‐6V#2S·6AL carrying PmV, was screened and proved to have a higher transmission rate than the original translocation T6V#4S·6DL, which will greatly promote the utilization of PmV. The above conclusions of this research will provide important guidance for utilization of Pm21 and PmV more effectively, in wheat powdery mildew resistance breeding.     

用遥感影像提取大别山区水稻种植面积——以Landsat 8为例

本研究选取安徽省安庆市大别山为研究区,包括太湖县、岳西县、宿松县和潜山县,选取2016年和2017年的6景Landsat 8卫星遥感影像,通过遥感影像提取水稻种植面积,分析大别山区的水稻种植面积分布,并对研究区域的水稻种植面积进行动态实时监测。用遥感解译方法分别提取了研究区内的晚稻种植面积,并利用随机点验证和Kappa系数验证结果精度。结果表明:2017年和2016年水稻种植面积的提取精度分别为93.44%、93.78%,Kappa系数分别为0.86、0.83,证明水稻提取效果精确;对比研究区域各个县内的2017年和2016年晚稻种植面积,发现安庆市大别山区太湖县、潜山县、岳西县的水稻种植面积变化率均在5%以内,属于正常变化,由于2016年宿松县遭遇了水灾,部分农田被淹没,故宿松县2017年水稻种植面积相比2016年增加了13.01%;对比传统的农作物种植面积统计方法,利用遥感的方法更省人力、物力、财力,并且能精确、快速地实现对农作物的实时动态监测。     

Role of cytokeratin 8 on changes of intestinal epithelial permeability induced by corticotropin-releasing factor

AIM: To investigate the role of cytokeratin 8 (CK8) on the change of intercellular permeability of intestinal epithelial cells induced by corticotropin-releasing factor (CRF). METHODS: The expression levels of CRF receptor 1 (CRFR1) and CRFR2 on human colon adenocarcinoma HT29 cell surface were determined by immunofluorescence staining. After treatment with 100 nmol/L CRF for 72 h, the translocation of FITC-labelled dextran was measured in a Transwell chamber. The structural changes of tight junctions were observed under transmission electron microscope. The expression levels of CK8, and tight junction proteins ZO-1 and occludin were determined by Western blot. The activity of protein kinase C (PKC) was detected by ELISA. Furthermore, the effects of CRF on intestinal epithelial permeability were examined in CK8-silencing HT29 cells, which were constructed by infection with sh-CK8 lentivirus. RESULTS: CRF treatment increased the permeability of FITC-labelled dextran (P<0.05), caused the opening of tight junctions, and induced increased fluorescence intensity of CK8. The expression levels of occludin and ZO-1 were down-regulated (P<0.05). PKC activity was decreased at 1 h after CRF treatment (P<0.05). CRF-induced increase in the permeability and down-regulation of occludin were not blocked by CK8 silencing. Nevertheless,CK8 silencing blocked the effects of CRF regarding the decrease in the expression levels of ZO-1 and the increase in PKC activity (P<0.05). CONCLUSION: CK8 may be involved in CRF-induced increase in intestinal epithelial permeability by inhibiting the activity of PKC, and there may be other signaling pathways involved.