猪多杀性巴氏杆菌的分离鉴定及生物学特性研究

用PCR方法配合生化鉴定，从有肺炎症状猪的肺脏及进行性萎缩性鼻炎(Progressive atrophic rhinitis，PAR)症状猪的鼻拭子中分离出66株多杀性巴氏杆菌(Pasteurella multocida，Pm)。然后做了药敏试验，并用PCR方法对这66株Pm进行分型及毒素基因的检测，用豚鼠皮肤坏死试验及小鼠致死试验对产毒素多杀性巴氏杆菌(Toxigenie Pasteurella multocida，T^ Pm)进一步鉴定。结果显示PCR鉴定与生化鉴定Pm结果完全一致；PCR分型表明有46株为D型Pm，18株为A型：Pm，1株为B型Pm，1株无法定型；有8株用PcR检测为T^ Pm；豚鼠皮肤坏死试验及小鼠致死试验对这8株T^ Pm的进一步鉴定也表明均为产毒素菌株。所鉴定的8株T^ Pm都为D型，都分离于有严重PAR症状的猪。     

CD117‐ and vimentin‐positive telocytes in the bovine teat sphincter

Mastitis is a common economically relevant problem in dairy farming. As the major entry for pathogens is the papillary duct, one of the first defence mechanisms is the teat sphincter. This sphincter shows a rhythmic contractility of yet unknown origin. Searching for possible modulatory pacemaker cells, teat sphincters of eight cows were stained immunohistochemically with antibodies against CD117 and vimentin and evaluated microscopically for the presence of telocytes. CD117‐ and vimentin‐positive telocytes with telopodes were found in close contact with smooth muscle cells. Our findings present a first evidence of telocytes in the teat of bovines.     

猪乳脂肪球表面因子8的克隆与表达分布

基于电子延伸序列,本试验成功克隆了猪乳脂肪球表面因子8（MFGE8）基因（GenBank登录号：EU559724）并进行了序列分析,同时利用RT-PCR方法分析了MFGE8基因的组织分布规律。序列分析结果表明MFGE8开放读码框全长为1296bp,编码431个氨基酸残基的多肽链,克隆的MFGE8基因与人、大鼠、小鼠、牛的同源性分别为71．9％、78．7％、79．1％、87．7％,推测的氨基酸序列的同源性分别为65．7％、74．0％、74．0％、84．5％。氨基酸序列N端含有Notch样“EGF”结构,C端含有FA58C结构域。构建的系统发育树显示猪与牛的亲缘关系最近。组织表达分析结果表明MFGE8在乳腺中表达最高,在大脑中表达量最低。     

棘孢木霉菌剂对黄瓜生理特性及产质量的影响

为改善黄瓜生理特性,保证其高产栽培,利用苗期和成株期盆栽试验,以无菌水为对照,分别施入103、104、105、106和107 cfu/g棘孢木霉(Trichoderma asperellum)菌剂,测定不同菌剂施用量后的黄瓜幼苗形态建成、生理指标、质量和产量性状.结果 表明:棘孢木霉菌剂不同施用量均能有效提高幼苗株高、...     

两种亚菊属植物挥发油及主要成分对赤拟谷盗幼虫的活性研究

本文旨在研究川甘亚菊和灌木亚菊挥发油及其主要成分(1,8-桉叶油醇、樟脑、马鞭草烯醇、冰片和桃金娘烯醇)对赤拟谷盗幼虫的活性作用。采用水蒸气蒸馏法提取挥发油并通过熏蒸、触杀和驱避等测试方法评价两种挥发油及其主要成分对赤拟谷盗幼虫的生物活性。结果表明,川甘亚菊挥发油、灌木亚菊挥发油和1,8-桉叶油醇均具有一定的熏蒸活性(LC₅₀分别为94.60、81.17 mg/L和18.42 mg/L)和触杀活性(LD₅₀分别为216.93、100.50μg/头和52.69μg/头)。同时,在高测试浓度(78.63 nL/cm~2和15.73 nL/cm~2)时,分别处理2 h和4 h后川甘亚菊和灌木亚菊挥发油以及桃金娘烯醇对赤拟谷盗幼虫均表现出较明显的驱避活性,冰片驱避活性接近于阳性对照避蚊胺。因此,川甘亚菊和灌木亚菊挥发油对赤拟谷盗的幼虫有一定的防治作用。     

Land surface temperature retrieval for arid regions based on Landsat-8 TIRS data:a case study in Shihezi,Northwest China

Scientific interest in geophysical information about land surface temperature （LST） is ever increasing, as such information provides a base for a large number of applications, including environmental and agricultural monitoring. Therefore, the research of LST retrieval has become a hot topic. Recent availability of Landsat-8 satel- lite imagery provides a new data source for LST retrieval. Hence, exploring an adaptive method with reliable ac- curacy seems to be essential. In this study, basing on features of Landsat-8 TIRS thermal infrared channels, we re-calculated parameters in the atmospheric transmittance empirical models of the existing split-window algorithm, and estimated the ground emissivity with the help of the land cover classification map of the study area. Further- more, a split-window algorithm was rebuilt by virtual of the estimation model of the updated atmospheric transmit- tance and the ground emissivity, and then a remote sensing retrieval for the LST of Shihezi city in Xinjiang Uygur autonomous region of Northwest China was conducted on the basis of this modified algorithm. Finally, precision validation of the new model was implemented by using the MODIS LST products. The results showed that the LST retrieval from Landsat-8 TIRS data based on our algorithm has a higher credibility, and the retrieved LST is more consistent with the MODIS LST products. This indicated that the modified algorithm is suitable for retrieving LST with competitive accuracy. With higher resolutions, Landsat-8 TIRS data may provide more accurate observation for LST retrieval.     

脱毒马铃薯‘冀张薯8号’冬种最佳密度试验研究

通过田间试验,探索‘冀张薯8号’冬种不同密度对其产量和经济性状的影响.结果表明:随着密度的增大其商品著率减少,不同密度对马铃薯产量影响较大,冬种马铃薯‘冀张薯8号’栽培最佳密度为4 200株/667 m2.     

早熟马铃薯新品种洛马铃薯8号的选育

洛马铃薯8号由“中薯3号x秦芋30号”杂交选育而成。早熟,生育期66d,休眠期中等,51d左右。薯块卵圆形,黄皮黄肉,薯皮光滑,芽眼浅,薯块较整齐,平均单株薯块数2．5个,单薯质量104．72g,商品薯率94．46％,淀粉含量13．6％,VC含量24．4m~100g,蛋白质2．70％,还原糖含量0．19％,较抗病毒病和晚疫病．667m2产量1302．04kg以上,适宜中原二季作区春秋两季种植。     

辣椒雄性不育三系品种江淮8号的选育

江淮8号是以辣椒雄性不育系A-921为母本、甜椒雄性不育恢复系C-097为父本配制的辣椒一代杂交新品种。该品种早中熟,植株高度约65 cm,植株开展度约60 cm,株型紧凑,首花节位10~11节,挂果集中,果实牛角形,青果绿色,果面光滑,果长度12 cm左右,果肩宽度6 cm左右,平均单果质量约65 g,辣味中等。667 m2产量约3 800 kg,抗疫病、炭疽病等病害,耐热耐湿,适应性强。