Response of labile organic C and N pools to plastic film removal from semiarid farmland soil

To examine the effects of plastic film removal on grain yield and soil organic matter (SOM), a spring maize (Zea may L.) field experiment was conducted for 5 yr at Changwu Agricultural and Ecological Experimental Station of Northwest China. Compared with traditional plastic film mulching during entire growing stages (FM), plastic film removal at the silking stage (RM) resulted in a 6.3% higher average maize yield. Under the RM treatment, soil organic carbon and total nitrogen significantly increased after the 5‐yr cultivation in the 0‐ to 20‐cm layer. Significant increases in extractable organic C (EOC), KMnO₄‐oxidizable C (KMnO₄‐C) and C management index (CMI) in the 0‐ to 20‐cm layer, and light fraction organic C and EOC in the 20‐ to 40‐cm layer were observed in response to plastic film removal after the 1‐yr treatment; the responses were more significant after 5 yr. Under the RM treatment, significant increases in microbial biomass C, light fraction organic N, extractable organic N, KMnO₄‐C and CMI were also observed after five years in the 20‐ to 40‐cm layer. Moreover, KMnO₄‐C and EOC were much more sensitive than other labile SOM fractions to the application of RM, even after only 1 yr of cultivation. Therefore, compared with mulching for the whole growing season, plastic film removal at the maize silking stage is an effective option for increasing yields and enhancing SOM concentration and soil sustainability in the regions with semiarid monsoon climates that have sufficient rainfall during maize reproductive stages.     

接种丛枝菌根真菌对玉米秸秆降解的影响

为研究接种丛枝菌根真菌(AMF)对玉米秸秆降解的影响,利用玉米秸秆为材料制成网袋,采用盆栽试验,以玉米(Zea mays L.)为宿主植物,分别接种Glomus intraradices和Glomus mosseae,于30,40,50,60d时收获后分析玉米秸秆降解量和C、N释放量,并运用Olson的指数模型Bt/B0=e-kt计算玉米秸秆及C、N的降解系数。数据表明,接种G.intraradices、G.mosseae显著提高了玉米秸秆降解量和降解系数,与不接种处理相比,分别高出5.21%,6.26%。C释放量、碳素降解系数也明显增加。接种处理减少了N释放量,且氮素降解系数随时间延长而下降。接种处理玉米秸秆的C、N降解系数不同直接反映了其降解速度的差异,进而影响了玉米秸秆的C/N,使秸秆更易于降解。研究结果显示出丛枝菌根真菌在生态系统氮循环中具有重要意义。     

苜蓿秸秆配施玉米秸秆还田对氮矿化、微生物生物量和酶活性的影响

秸秆的质量是影响秸秆还田后氮素矿化的重要因子.玉米秸秆C/N比较高,还田后往往造成对土壤氮素的固持,因此秸秆直接还田要补施氮肥,而在如今的农业生产中为满足对粮食产量的需求,氮肥的施用越来越多,不仅增加了生产成本还危害了土壤质量.在秸秆还田条件下,如何能够更加科学合理地施用氮肥是一个关键问题.该试验在可控条件下,研究苜蓿秸秆和玉米秸秆共同还田对氮矿化、土壤微生物生物量碳氮和酶活性的影响,比较豆科作物秸秆或者氮肥配施玉米秸秆在相同C/N下(25:1)还田对土壤供氮、土壤微生物生物量和酶活性的影响.室内实验共设置4个处理:(1)对照(CK);(2)玉米秸秆单施(M);(3)苜蓿秸秆配施玉米秸秆调节C/N为25:1 (MM);(4)无机氮肥配施玉米秸秆调节C/N为25:1(MF),在25℃下共培养270 d.玉米秸秆单施在整个培养过程中均表现为对土壤氮素的固持作用,苜蓿秸秆与氮肥配施米秸秆均缓解了土壤微生物对土壤氮素固持作用,提高了土壤无机氮含量,苜蓿秸秆配施相比于氮肥配施延长了氮素的可利用性.苜蓿秸秆与氮肥配施提高了土壤微生物生物量碳氮含量以及转化酶和β-葡萄糖苷酶活性,并且以苜蓿配施处理最高.与玉米秸秆单施相比,苜蓿秸秆配施对脲酶活性影响没有差异,氮肥配施显著降低了脲酶活性.相关性分析表明,氮输入量显著影响了土壤无机氮含量、土壤微生物生物量和酶活性.相比于氮肥配施玉米秸秆还田,苜蓿秸秆配施玉米秸秆还田具有较高的土壤无机氮含量、土壤微生物量和酶活性.因此,我们认为相比于氮肥配施玉米秸秆还田,苜蓿秸秆配施玉米秸秆还田是一个比较好的农业生产实践模式.     

Estimating Soil Carbon,Nitrogen, and Phosphorus Mineralization from Short‐Term Carbon Dioxide Respiration

The measurement of soil carbon dioxide (CO₂) respiration is a means to gauge biological soil fertility. Test methods for respiration employed in the laboratory vary somewhat, and to date the equipment and labor required have limited more widespread adoption of such methodologies. A new method to measure soil respiration was tested along with the traditional alkali trap and titration method. The new method involves the Solvita gel system, which was originally designed for CO₂ respiration from compost but has been applied in this research to soils with treatments of increasing dairy manure compost. The objectives of this research are to (1) examine the relationship between the CO₂ release after 1 day of incubation from soils amended with dairy manure compost that have been dried and rewetted as determined using the titration method and the Solvita gel system, and (2) compare water‐soluble organic nitrogen (N), as well as carbon (C), N, and phosphorus (P) mineralization after 28 days of incubation with 1‐day CO₂ release from the titration method and Solvita gel system. One‐day CO₂ from both titration and the Solvita gel system were highly correlated with cumulative 28‐day CO₂ as well as the basal rate from 7–28 days of incubation. Both methods were also highly correlated with 28‐day N and P mineralization as well as the initial water‐extractable organic N and C concentration.

The data suggest that the Solvita gel system for soil CO₂ analysis could be a simple and easily used method to quantify soil microbial activity and possibly provide an estimate of potential mineralizable N and P. Once standardized soil sampling and laboratory analysis protocols are established, the Solvita method could be easily adapted to commercial soil testing laboratories as an index of soil microbial activity.     

纽荷尔脐橙成年态节间茎段遗传转化研究

建立柑橘成年态节间茎段的离体再生和遗传转化系统是实现柑橘转基因育种实用化的前提基础。本试验以纽荷尔脐橙成年态节间茎段为试材,初步建立了脐橙成年态节间茎段的离体再生和遗传转化系统。结果表明采用改良的预处理灭菌法对成年态茎段进行消毒处理,污染率明显降低。节间茎段在MS＋3mg／L6-BA的培养基上,不定芽再生率较高。采用根癌农杆菌介导法将外源抗真菌病基因chit42导入其中,抗性芽通过二次嫁接后获得再生植株,PCR检测4株抗性植株中有2株扩增出该特异性条带,RT-PCR分析进一步确认外源基因chit42能够在转基因植株的基因组中表达。     

DNA profiling of Capsicum landraces of Manipur

Seven chilli landraces of Manipur belonging to three cultivated species of Capsicum (Capsicum annuum, Capsicum frutescens, and Capsicum chinense) form economically important food crops of the region. The genotypes were characterized using ten random amplified polymorphic DNA (RAPD) markers. The cluster analysis based on Jaccard's similarity coefficient calculated by UPGMA method differentiated the genotypes into two main cluster groups. One cluster represented the C. annuum genotypes while the other cluster represented the C. frutescens and the C. chinense genotypes. C. chinense genotypes were more close to C. frutescens genotypes. Genetic variation between the C. frutescens genotypes was more than among the C. annuum genotypes and the C. chinense genotypes were the least similar ones.     

基于16S rRNA和Cyt b基因序列探讨2种梅童鱼的遗传分化

比较分析了棘头梅童鱼（Collichthys lucidus）和黑鳃梅童鱼（C.niveatus）的16SrRNA和Cytb基因片段序列差异及遗传分化程度。在长度为526bp的16SrRNA和379bp的Cytb基因片段的核苷酸序列中,2种间共检测到44处核苷酸替代。分析结果表明,2个基因片段的鸟嘌呤（G）含量较低,在Cytb蛋白质编码基因第三密码子位点上表现尤为明显。基于16SrRNA和Cytb基因片段分析结果显示,2种间平均遗传距离分别为0．012和0．111。构建的系统树显示2种梅童鱼在这2个基因片段上存在显著的遗传分化。根据Cytb基因2％／百万年的进化速率推断,棘头梅童鱼与黑鳃梅童鱼的分化时间约为550万年,发生于上新世（Pliocene）早期。     

不同施肥措施对稻田土壤温室气体排放的影响

选取江西红壤性双季稻水稻土为研究对象,采用盆栽模拟试验研究了4种不同施肥措施即当地农民习惯施肥(FP)、较FP减施20%化肥氮且有机肥替代20%化肥氮(T1)、在T1基础上加施Si、Zn、S三种微肥(T2)和在T2基础上采用20%缓释氮肥替代普通化肥氮(T3)对稻田主要温室气体CO2、CH4和N2O排放的影响,并对土壤微生物量碳(SMBC)、土壤微生物量氮(SMBN)、水稻产量的影响进行了分析。结果表明:4种处理稻田土壤CO2的总排放通量均无显著性差异;稻田土壤N2O的总排放量与FP处理相比,T1、T2和T3处理均有显著性减少(P<0.05),分别减少了31.72%、27.17%和43.65%,T3较T2处理显著减少22.83%(P<0.05);稻田土壤CH4的总排放量与FP处理相比,T1、T2、T3处理分别高了13.06%、13.90%、21.97%,其中T3处理差异达到显著水平(P<0.05)。与FP处理相比,T1、T2、T3处理显著提高了SMBC和SMBN的含量(P<0.05),分别提高了18.91%、19.30%、20.07%和28.95%、31.66%、29.96%;T1、T2、T3处理对水稻产量均无显著性影响。稻田土壤CH4和N2O的排放与SMBC和SMBN存在显著的相关性(P<0.01)。总体看,T3处理在降低N2O的总排放量的同时对提升土壤SMBC和SMBN含量具有明显作用。     

黄土旱区土壤C/N调控对谷子根系形态及产量的影响

为明确土壤C、N配施调控旱地谷子根系形态构建及产量形成的关联机制,研究以晋谷21谷子品种为试验材料,采用大田试验（处理小区面积30 m²）,设置不同C/N调控处理：CK（N 21.7 kg/hm²);A（N 21.7 kg/hm², C 6.7 kg/hm²）;B（N 32.7 kg/hm², C 6.7 kg/hm²）;C（N 43.3 kg/hm², C 6.7 kg/hm²）;D（N 54.3 kg/hm², C 6.7 kg/hm²）,N为含量为46%的尿素,C为玉米秸秆生物炭,系统调查了谷子根系形态指标体系和产量构成因子的变化规律。试验结果表明：①土壤CN合理配施可以有效增加谷子的产量,增产效果以处理C最为显著,较CK 处理显著增加44.9%;②同等生物炭施用水平下,充足或过量的N会抑制根系伸长下扎,但有助于根系的横向生长和体积膨大,处理B对根系总长、总表面积、总体积都有显著的促进作用,分别提升4.75%、13.92%、17.02%。③同等生物炭施用水平下,增施氮肥会减少土壤含水量,其中B处理较CK处理土壤水分含量显著降低4.2%;④增加氮素的施用可以有效促进谷子在抽穗期和灌浆期的根系生长发育,促进根系干物质的积累,与CK处理相比,A、B、C三个处理抽穗期和灌浆期谷子根系的干物质都显著性增加,增幅分别为28.13%、35.93%和24.75%,灌浆期增幅分别为36.83%、46.35%、49.52%;⑤配施生物炭可有效调控土壤电导率和土壤温度随作物生育期和气候环境的变化而变化,与CK相比,B处理显著降低土壤电导率19.5%,D处理显著提升了3%的土壤温度,对根系形态发育和功能发挥具有显著影响。综上所述,合理的CN配施调控可以通过改善土壤水肥环境,优化根系空间分布格局和功能,实现谷子产量和水分利用效率同步提升。     

Legume–barley intercropping stimulates soil N supply and crop yield in the succeeding durum wheat in a rotation under rainfed conditions

Legume–cereal intercropping is increasingly being appreciated in dryland areas, where severe climatic conditions and intensive agricultural practices, generally dominated by continuous cereal cultivation, determine depletion of soil nutrient resources and decline of soil fertility. This research aimed to assess whether and to what extent a newly introduced legume-based intercropping system is able to ameliorate the biological fertility status of an arable soil in a way that is still noticeable during the succeeding durum wheat cropping season in terms of changes in bacterial community structure, soil C and N pools, and crop yield. A field experiment was carried out under rainfed conditions in Southern Italy on a sandy clay loam soil cultivated with durum wheat following in the rotation a recently established grain legume (pea, faba bean)–barley intercropping. Soil chemical, biochemical and eco-physiological variables together with compositional shifts in the bacterial community structure by LH-PCR fingerprinting were determined at four sampling times during the durum wheat cropping season. Soil fertility was estimated by using a revised version of the biological fertility index. Results showed that even though the microbial biomass was significantly altered, the preceding legume intercrops stimulated C-related functional variables thus leading to an increased release of mineral N, which was larger in crop treatments succeeding pea-based than faba bean-based intercropping. The increased N made available in soil enabled the succeeding durum wheat to achieve an adequate grain yield with a reduced N-fertilizer use. Soil type and environmental conditions rather than crop treatments were major determinants of bacterial community structure. The biological fertility status was not varied, suggesting that in intensively managed rainfed areas long-term crop rotations with intercropped legumes are needed to consistently ameliorate it.