Ocular melanosis in the Cairn Terrier: histopathological description of the condition, and immunohistological and ultrastructural characterization of the characteristic pigment-laden cells

Objective  To describe the microscopic features and lineage of proliferating/infiltrating pigmented cells in ocular melanosis of Cairn Terriers.
Animals studied  Forty-nine globes removed from 45 Cairn Terriers with ocular melanosis and three globes from control dogs were available for microscopic examination.
Procedures  All globes were examined histologically, eight affected and three control globes were also examined by immunohistochemistry, and three affected and three control globes by transmission electron microscopy.
Results  Large round pigment-laden cells infiltrated the anterior uvea, obscured the drainage angle and were present within the sclera and episclera of affected globes. Similar pigmented cells were present in lower numbers in the posterior segment of the globe, the optic nerve meninges and periphery of the optic nerve. Changes due to chronic glaucoma were present in many globes and some had evidence of uveitis. Many of the pigmented cells were immunoreactive to HMB45 and some were MITF and vimentin positive. One globe, which was inflamed when removed, had many pigmented cells that were CD18 immunoreactive. The other eyes had lower numbers of CD18 positive cells. The pigmented cells were not immunoreactive to smooth muscle actin, S-100, MART/Melan A, chromogranin A/B, PGP 9.5, synaptophysin, MNF116, AE1/AE3, and CD45. Ultrastructurally many of the pigmented cells had features typical of melanocytes while a smaller number appeared to be melanophages.
Conclusions  Ocular melanosis in Cairn Terriers is characterized by an infiltration of pigment-laden cells predominantly, but not exclusively, within the anterior uvea and anterior sclera. Most of these cells appear to be melanocytes although a variable proportion are pigment-laden melanophages.     

高效利用木质素·半纤维素的草菇菌株初筛试验

[目的]为筛选出可高效利用木质素、半纤维素的草菇菌株,以促进草菇生产。[方法]以V9、V11、V广、V展1、V展2、V信丰6个草菇菌株进行透明圈法测定,愈创木酚氧化显色观测,过氧化酶显色观测。[结果]6个菌株均能利用木质素、半纤维素,但其能力有差异。V展1、V信丰能高效利用木质素、半纤维素。[结论]从草菇品种中筛选了可高效利用木质素、半纤维素的菌株V展1和V信丰,对促进草菇生产有积极的意义。     

不同品种种公牛繁殖性能分析

[目的]在相同的饲养环境条件下,分析不同品种种公牛之间繁殖性能及适应性的差异。[方法]通过对云南省家畜冷冻精液站四个品种的种公牛相关精液品质测量数据及终生冻精产量数据进行对比分析。[结果]经过对16头种公牛繁殖性能及适应性分析,荷斯坦种公牛原精活力及冻后活力都较其它种公牛表现出较明显的优势;荷斯坦种公牛在有效采精率及冻精产量上也明显高于其它品种公牛。     

温度对奶牛冻精解冻后活力的影响

本研究对奶牛冻精解冻后在4℃,15℃,25℃温度下分别保存2 h,4 h,6 h,8 h,10 h的活力进行了测定。结果表明,4℃和15℃保存到10 h精子活力0.381±0.0170和0.379±0.0197,与初解冻接近(P0.05);25℃保存随时间延长精子活力呈下降趋势,和初解冻相比,6 h为0.349±0.0223(P0.05),8 h和10 h分别为0.302±0.0215和0.263±0.0287(P0.01)。牛冻精解冻后在4℃和15℃保存10 h以内,精子活力仍然维持在一个较高水平,符合输精要求,在此温度和时间范围内进行长距离携带,对精液质量影响小,可以达到预期的输精效果。环境温度在25℃左右时,冻精解冻后宜于4 h以内进行输精。     

影响种公牛精液品质因素的研究

[目的]本文旨在分析种公牛精液品质的影响因素,以便进一步提高种公牛冷冻精液质量。[方法] 对2010年至2020年采精记录种公牛的射精量、原精活力、精子密度、解冻活力进行统计分析。[结果] 笔者对影响牛精液质量的主要因素进行了研究。结果发现,不同种之间、不同品种之间、不同品系之间、不同年龄、不同季节之间、不同饲养和管理方法之间,公牛的精液质量存在显著差异。 [结论] 遗传、气候环境、饲养管理等因素影响种公精液质量。     

早胜牛肉用性能测定报告

选择18月龄早胜牛公牛6头,24月龄母牛4头屠宰,测定产肉性能,观察其产肉性能提高状况和了解不同性别的产肉特点。结果表明,18月龄公牛除屠宰率和净肉率与1982年测定结果差异不显著(P<0.05)外,胴体重、净肉重、胴体产肉率、肉骨比、肉脂比和眼肌面积测定结果均极显著(P>0.01)高于1982年测定结果。另外虽然公牛月龄小于母牛6个月,但净肉率、肉脂比和眼肌面积测定结果均显著地高于母牛,同时肉中脂肪含量显著低于母牛。     

平凉红牛品牌提升与发展策略研究

为了解决平凉红牛品种选育缓慢、品牌知名度低等短板,本文介绍了平凉红牛的发展现状,提出了平凉红牛品牌提升的思路和发展策略,旨在加大平凉红牛的产业化开发力度,为打响平凉红牛品牌,发展平凉红牛产业奠定坚实的基础。     

Ultrasonographic Appearance of Dandy Walker-Like Syndrome in a Boston Terrier

Congenital cerebellar disorders can be caused by in utero or neonatal infections, genetic aberrations causing malformations, and neurodegenerative processes. Most congenital cerebellar disorders are diagnosed definitively with histopathology. However, antemortem diagnosis of cerebellar malformations can be made by imaging the brain. This paper describes the antemortem appearance of a congenital cerebellar malformation in a Boston Terrier puppy on ultrasound images. This appearance was compared with the postmortem findings that were comparable to Dandy Walker Syndrome in humans. A previous report of this syndrome in Boston Terriers suggests the problem may be inherited in this breed.     

猴头菌栽培模式研究

应有正交试验设计Ｆｕｚｚｙ分析法,研究了猴头菌袋料栽培中不同菌株、出菇方式及料袋袋宽对生物学效率的影响。结果表明,菌株Ｈ８９０８、袋宽１８０ｍｍ的料袋、两端出菇的组合为最佳栽培模式。以此模式栽培猴头菌,生长学效率达１６５％。