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排序方式: 共有111条查询结果,搜索用时 31 毫秒
61.
阿拉尔地区野生墨汁鬼伞生物学特性研究初报 总被引:1,自引:0,他引:1
阿拉尔地区野生墨汁伞可用一般食用菌的培养方法进行培养,繁殖,其菌丝体生长所需的温度范围为4℃-30℃,最适生长温度为20℃-25℃左右,PH值范围为5-10,最佳为6-8,是一种怕光、需氧生长的菌丝体,其子实体是一种中高温型食用菌。 相似文献
62.
Hoffmann G van den Ingh TS Bode P Rothuizen J 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2006,20(4):856-861
This study summarizes the clinical and pathologic findings in 15 Labrador Retrievers with copper-associated chronic hepatitis (CACH). Our hypothesis was that this form of hepatitis is caused by a defect in hepatic copper metabolism, which most likely originates from a genetic defect. Affected Labradors consisted of 11 female and 4 male Labrador Retrievers. Eight family members of 2 of these patients were examined prospectively, as were 6 unrelated healthy Labrador Retrievers. All dogs were registered at the breed club. The average age at clinical presentation was 7 years (range, 2.5-10.5 years). All dogs were presented for anorexia, which was associated with vomiting in 8 patients. The diagnosis of CACH was based on histologic examination of liver biopsy specimens in all dogs, including semiquantitation of copper. A disproportionate increase in alanine aminotransferase (ALT) activity relative to alkaline phosphatase (ALP) activity, as well as the centrolobular localization of copper and the association of copper accumulation with hepatic lesions, suggested a primary copper storage disease rather than primary cholestatic liver disease causing copper accumulation. Mean hepatic copper concentration measured in related Labradors was 1,317 microg/g dry weight liver (range, 402-2,576 microg/g). Mean hepatic copper concentration of unrelated normal Labradors was 233 microg/g dry weight liver (range, 120-304 microg/g). Our findings support the hypothesis that a hereditary form of hepatitis occurs in Labrador retrievers and is caused by a defect in hepatic copper metabolism. 相似文献
63.
M. Trotta E. Carli G. Novari T. Furlanello L. Solano-Gallego 《Veterinary parasitology》2009,165(3-4):318-322
A 4-year-old intact female American Pit Bull Terrier from Italy descendant of an American-born bitch was evaluated for anorexia, lethargy, weakness, and intermittent vomiting. On physical examination, the dog was dehydrated, had pale mucous membranes, hunched posture and abdominal pain. A moderate anemia was observed. Splenomegaly and hyperechoic regions suspected as infarcts in the spleen were seen on abdominal ultrasound. Based on the suspicion of splenic torsion, splenectomy was performed. After surgery, the clinical condition deteriorated. A follow-up complete blood count demonstrated severe macrocytic normochromic anemia with evidence of marked regeneration, left shift neutrophilia, monocytosis and marked thrombocytopenia. Blood smear evaluation revealed single to multiple, variable sized (1–3 μm in diameter), and round to oval to band-like piroplasms within many red blood cells consistent with small form Babesia spp. or Theileria spp. A partial segment of the 18S rRNA gene was amplified and the PCR product was analyzed by direct sequencing. The nucleotide sequence was completely identical to that of Babesia gibsoni present in GenBank®. This is the first molecular detection and characterization of B. gibsoni infection in a sick dog from Italy. 相似文献
64.
采用正交试验方法对美味蘑菇原生质体制备与再生最佳体系进行筛选,包括酶条件、渗透压稳定剂、菌龄、酶解的时间及温度。结果表明,原生质体最佳制备条件为液体静置培养12d菌丝体,0.6mol·L-1NaCl作为渗透压稳定剂,在2%溶壁酶和3%蜗牛酶作用下,28℃酶解3h,原生质体制备率为3.6×106个·mL-1;最佳再生条件为液体静置培养12d菌丝体,0.6mol·L-1MgSO4·7H2O作为渗透压稳定剂,在2%溶壁酶和1%蜗牛酶作用下,35℃酶解1.5h,原生质体再生率为3.01%。 相似文献
65.
[目的]为了掌握口蹄疫疫苗的过敏反应情况.[方法]在给36头种公牛注射口蹄疫疫苗的同时,注射Vc注射液.观察了注射后种公牛体表反应特征,进行了注射前后一个月内冻精质量对比,运用SAS软件进行统计分析.[结果]显示:种公牛无过敏反应.[结论]此项研究对于种公牛注射口蹄疫疫苗具有实际操作参考价值. 相似文献
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68.
为探究外生菌根真菌的发酵条件,并进行杨树溃疡病的田间防治试验。以红绒盖牛肝菌[ Xerocomus chrysenteron (Bull.: Fr.) Quél]为实验材料,分析不同的接种量、溶解氧及pH值对其液体发酵的影响,发酵产物并用于杨树溃疡病的田间防治。结果表明:接种量7.2%,发酵中后期溶解氧浓度不低于33%,培养基pH保持5.5左右时可有效提高红绒盖牛肝菌菌丝生物量,且缩短了发酵周期;灌根法研究红绒盖牛肝菌对杨树溃疡病的田间防治效果,防治效果可达到54.5%。该实验所得出的红绒盖牛肝菌发酵条件和野外防治数据可为今后该菌株田间应用提供参考。 相似文献
69.
为研究小刺猴头菌[Hericium caput-medusae(Bull.:Fr.)Pers]发酵浸膏多糖的抗氧化活性。以工厂化小刺猴头菌发酵浸膏为原料,采用透析法分级,DEAE sepharose fast flow柱层析纯化,分别获得粗多糖组分HFCP1和中性多糖HFCP1-1;通过PMP柱前衍生-高效液相色谱法和红外光谱法对单糖组成和结构进行分析,单糖组成均以葡萄糖和半乳糖为主。化学法检测多糖抗氧化活性,结果显示:HFCP1和HFCP1-1清除羟基自由基、超氧阴离子自由基、2,2′-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate,ABTS+)和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)的IC50值分别为7.66和6.77mg/mL、6.16和5.30mg/mL、0.63和0.85mg/mL、0.39和2.62mg/mL。以RAW264.7细胞建立H2O2诱导损伤模型,细胞抗氧化结果表明:HFCP1和HFCP1-1处理组与模型组相比均显著增强细胞活力(P0.05),浓度为100和200mg/L时MDA含量与模型组相比均显著下降(P0.05);HFCP1浓度为100和200mg/L时与模型组相比显著增加SOD和GSH-Px的活性(P0.05);HFCP1-1浓度为200mg/L时与模型组相比显著增加SOD活性(P0.05);也能增加GSH-Px的活性,但未达到显著水平(P0.05)。 相似文献
70.
对不同冻精稀释液配方进行对比试验,结果表明,配方Ⅰ和配方Ⅱ对奶牛冻精的活力效果基本一致,对西门达尔牛冻精的活力效果配方Ⅱ稍好于配方Ⅰ。在对冻精顶体完整率的对照试验中发现,荷斯坦牛和西门达尔牛都表现出配方Ⅲ明显好于配方Ⅱ。 相似文献