猪源大肠杆菌O157∶H7耐药表型和消毒剂抗性调查

为了调查广西猪源大肠杆菌O157∶H7分离株的耐药表型和消毒剂抗性情况,本研究测定5株猪源大肠杆菌O157∶H7广西分离株的药物敏感性和消毒剂抗性,并应用PCR对5株细菌的耐氨基糖苷类抗生素基因:氨基糖苷磷酸转移酶基因aph(3)-Iia、乙酰转移酶基因aadA和aadB进行扩增。27种抗菌药物的敏感结果表明,5株菌株只对氟苯尼考、头孢曲松、头孢西丁和头孢噻肟敏感;对罗红霉素、多黏菌素B、利福平、林可霉素、阿莫西林、氨苄西林和头孢噻吩的耐药率为100%;对壮观霉素、链霉素、头孢拉定等药物的耐药率在20%~60%之间。在5株大肠杆菌O157∶H7广西分离株中,分别有1株、1株和3株菌株对23种、11种和9种抗菌药产生耐药性。PCR结果证实,5株细菌携带有耐氨基糖苷类抗生素基因,耐药基因的存在与其耐药表型有一定的相关性。消毒剂抗性结果显示,5株细菌对聚维酮碘溶液、新洁尔灭消毒液、稀戊二醛溶液、双季铵盐—碘消毒液、复方过氧乙酸具有抗性,只对二氯异氰尿酸钠粉敏感。研究结果对预防和控制广西大肠杆菌O157∶H7提供了数据。     

Correlation between expression of ALDH1/ABCG2 and microvessel formation in epithelial ovarian cancer

AIM: To elucidate the correlation between the expression of aldehyde dehydrogenase 1 (ALDH1)/ATP-binding cassette subfaminly G member 2 (ABCG2) and microvessel density (MVD) in epithelial ovarian cancer (EOC).METHODS: In 198 specimens of EOC and 60 specimens of ovarian benign epithelial tumor tissues, the protein expression of ALDH1/ABCG2 and CD105 (microvessel marker) was detected by immunohistochemical staining.RESULTS: The positive rates of ALDH1 and ABCG2 in the EOC were 64.1% and 61.6%, respectively, while the positive rates in benign epithelial tumor tissues were 8.3% and 6.7%, respectively, and there were significant differences between them (P<0.05). In EOC and benign epithelial tumor tissues, the MVD were 22.6±9.7 and 5.03±3.35, respectively, and the difference was also significant (P<0.05). The expression of ALDH1 and ABCG2 in EOC was significantly related to differentiation, FIGO stage,and abdominal organ and lymph node metastasis (P<0.05). MVD had correlation with differentiation, FIGO stage, ascite, and abdominal organ and lymph node metastasis (P<0.05). MVD had positive correlation with the expression of ALDH1 and ABCG2 (P<0.01). There was also a positive correlation between the expression of ALDH1 and ABCG2 (P<0.01). Over-expression of ALDH1/ABCG2 and MVD≥23 were related to the poor prognosis. The survival rates in ALDH1/ABCG2 positive and MVD≥23 groups were significantly lower than those in ALDH1/ABCG2 negative and MVD<23 groups (P<0.05). The FIGO stage, the expression of ALDH1/ABCG2 and MVD were indepen-dent prognosis factors of EOC (P<0.05).CONCLUSION: The results suggest that the expression of ALDH1/ABCG2 and MVD in EOC are related to differentiation, lymph node metastasis, clinical stage and prognosis. Combined detection of these indexes may play an important role in predicting the progression and prognosis of EOC.     

多效土壤消毒剂在蔬菜育苗中的应用

多效土壤消毒剂对蔬菜育苗苗床进行熏蒸消毒 ,可以有效降低苗期病害发生率、地下害虫虫口密度和杂草数量 ,并显著促进苗期生长发育 ,提高其壮苗指数     

栝楼组织培养研究

为解决籽用栝楼高品质种苗的不足,选用无毒、结籽率高并生长健壮的栝楼植株为材料,进行组织快繁。通过对栝楼不同外植体的选取、消毒方法、愈伤组织诱导、丛生芽诱导、继代和试管苗生根等相关条件研究,探讨栝楼组织快繁的技术方法。结果表明：以雌性栝楼4—5 月萌生的栝楼芽适宜做外植体,外植体用75%乙醇30 s,无菌水冲洗3 次,0.31% ClO2处理5 min,无菌水冲洗4~5 次效果好。适宜芽诱导的培养基为MS+1 mg/L 6-BA+ 0.3 mg/L NAA;继代培养基为MS+ 0.3 mg/L 6-BA+ 0.1 mg/L NAA和MS+0.4 mg/L 6-BA+ 0.2 mg/L NAA;栝楼根诱导培养基为MS+0.1 mg/L IBA和MS+0.1 mg/L NAA。     

In vitro primary porcine alveolar macrophage cell toxicity and African swine fever virus inactivation using five commercially supply compound disinfectants under various condition

Efficacy of African swine fever virus (ASFV) inactivation using five commercially supply compound disinfectants was evaluated under various condition. Virucidal efficacy demonstrated that products A and E could inactivate at 1:800 within 1 min for both temperatures, while products B, C and D inactivated at 1:400. However, product D could inactivate at 1:800 when the exposure time was extended to 30 min and effected only 20°C. In addition, the cytotoxicity demonstrated that products A, B, C, D and E did not significantly affect to cell at 1:51,200, 1:12,800, 1:12,800, 1:25,600 and 1:12,800 dilution, respectively. In conclusion, these disinfectants could inactivate ASFV, however, the application of these products should be performed under safety precautions to prevent cytotoxicity in humans and animals.     

过氧乙酸戊二醛复方消毒剂与氨气的互作

为研究氨气与过氧乙酸戊二醛复方消毒剂(简称复方消毒剂)相互中和的效果及氨气对消毒效果的影响,通过测定不同剂量512倍稀释的复方消毒剂,在不同作用时间、不同氨气浓度时,喷雾消毒前后气雾柜内细菌数量和氨气质量浓度,计算杀菌率和氨气中和率,以确定512倍稀释的复方消毒剂对氨气的中和效果及高浓度氨气对其杀菌效果的影响.结果显示...     

得克斯消毒剂室内抑菌效果与应用研究

研究了食用菌接种和糙皮侧耳（Ｐｌｅｕｒｏｔｕｓ ｏｓｔｒｅａｔｕｓ）生料栽培过程中得克斯消毒剂的抑菌效果。结果表明,Ｂ型配方（３ｇ／ｍ＾３）烟熏消毒效果达１００％,Ｄ型配方（０．１％）对木霉等５种杂菌的抑菌效果均达１００％,对糙皮侧耳菌株ＣＣＥＦ－８９无抑制作用。栽培试验表明,Ｄ型配方（０．１％）拌料处理,污染率〈１％,比多菌灵处理增产２３．９％;喷施０．１％～０．１５％Ｄ型药液,防病效率比常用消     

新型水产养殖消毒剂的制备、性能及其临床应用Ⅰ．碘伏

研究了新型水产养殖消毒剂——碘伏的制备、稳定性、毒性和消毒效果 .通过产品的理化性能、毒理、定量杀菌效果等试验了解产品性能 .结果表明 :该产品稳定 ,定温下有效期至少 1a;在使用体积分数范围内 ,对鳗鱼、甲鱼无刺激性 ;对家畜 L D50 >50 0 0 mg· kg-1,对欧洲鳗、日本鳗、甲鱼的 L D50 分别为 144 5、1750、3975mg· kg-1;2 2℃下 ,对嗜水性单胞菌最低杀菌体积分数为 1.0× 10 -6,杀菌率达 10 0 % ,在 3.0× 10 -6的体积分数下作用 4 h;对日本鳗爱德华氏病治疗有效率达 80 % ,对欧洲鳗赤鳍病治疗有效率达 85% .适当提高温度和酸度可提高消毒杀菌效果     

安全型奶牛消毒剂牧场应用效果的研究

本文对安全型奶牛消毒剂牧场的应用效果进行了研究,以碘伏为对照,通过菌落总数、金黄色葡萄球菌数以及大肠杆菌等指标的测定推断出本消毒剂最佳药剂组合配比,并在此基础上,测定了消毒剂使用前后的体细胞数变化。本消毒剂高效、持久并能有效降低体细胞数目,可预防奶牛乳房炎症的发生,提高生奶的质量控制水平。