线粒体复合体Ⅰ呼吸抑制剂的研究

线粒体复合体Ⅰ呼吸抑制剂不仅在医药上有着重要的研究价值,在农药方面也有着特殊的意义。这类药剂的作用机制比较特殊,害虫不易产生抗性,是一类非常有前途的杀虫药剂。从植物,微生物等生物体中发现了许多线粒体复合体Ⅰ呼吸抑制剂,如鱼藤酮、粉蝶霉素A、辣椒碱,番荔枝内酯、myxalamid等,它们可以作为农药的先导化合物进行药物合成。根据不同的作用方式,线粒体复合体Ⅰ呼吸抑制剂可分3种类型,分别以粉蝶霉素A、鱼藤酮、辣椒碱为代表。     

免疫抑制剂作用机理的研究进展

20世纪以来,科技工作者对硫唑嘌呤、咪唑立宾、环孢素等免疫抑制剂的作用机理进行了大量研究,本文综述了近年来用于临床或处于临床试验阶段的免疫抑制药物的作用机理研究进展.     

加大建设新农村的农业财政投资力度研究

农业是维持我国社会稳定和国民经济持续健康发展的基础产业，农业具有“准公共产品”属性，决定了政府必须介入农业领域进行投资与保护，农业财政投资与农业发展密切相关。论文在对财政支持与农业发展理论思考的基础上，针对当前我国农业财政投资的现状和存在的问题进行了研究，对产生问题的宏观制度性根源进行了分析，并提出了相应的政策措施。     

Mechanisms Involved in the Growth Promotion of Weaned Piglets by High-level Zinc Oxide1

Dietary supplementation with high lev- els of zinc oxide （ZnO） is an effective method to prevent diarrhea and promote growth in weaned pig- lets. However, the mechanisms behind these beneficial effects are not well understood. Recent advances in this field indicated that the effects of ZnO on piglet growth are achieved through multiple regulatory path- ways rather than a single pathway. This review focu- ses on recent advances in the effects of high level ZnO on： 1 ） Zn status in weaned piglets; 2 ） intestinal microbe flora regulation; 3 ） intestinal structure and function;4） ion secretion in the intestine epithelium;5） intestinal immunology; and 6） secretion of brain-in- testine peptides. ZnO appears to regulate the intestinal microflora and decreases the release of histamine to prevent diarrhea. ZnO also increases the expression of intestinal insulin-like growth factor-1 （ IGF-1 ） and the IGF-1 receptor to ameliorate intestinal injury associat- ed with weaning, and mediates secretion of brain-gut peptides to stimulate food intake, to promote the growth of piglets. Better understanding of the underly-1 ing mechanisms should inspire us to supplement the diet for weaned piglets with ZnO and investigate new approaches to prevent diarrhea and enhance growth.     

β-折叠抗菌肽的研发及应用策略

抗生素的滥用导致全球细菌耐药性问题愈发严重,严重威胁人类、畜禽健康及畜牧业发展,功能多样且不易导致细菌产生耐药性突变的抗菌肽（antimicrobial peptides,AMPs）逐渐发展为抗生素的潜在替代品。β-折叠是抗菌肽一个主要的二级结构分类,该类肽通常由一个或多个二硫键来维系结构的稳定,现已发现许多抗菌肽具有此类结构。相比于目前研究广泛的α-螺旋AMP,它们被认为拥有更强的抗酶解能力和更高的细胞选择性。本篇综述介绍了β-折叠抗菌肽的来源和抗细菌机理,并梳理了一些常见的分子设计方法和应用策略,以期为β-折叠抗菌肽的研发应用提供新思路。     

酪酸梭菌的作用机理及其在动物生产中的应用

本文综述了酪酸梭菌的作用机理及其在动物生产中的应用。     

热应激对畜禽胃肠道的影响及机制

本文就热应激对畜禽胃肠道结构和功能的影响及其机制最新研究进展作一综述。     

复方二氯异氰尿酸钠和双季铵碘中和剂中和效果考察

采用悬液定量杀菌试验程序，对两种中和剂配方进行了中和剂鉴定试验，以便对复方二氯异氰尿酸钠和双季铵碘两种消毒剂的消毒效果进行评价。试验结果表明，中和剂配方Ⅰ（含3％吐温-80、0．5％硫代硫酸钠）对复方二氯异氰尿酸钠，中和剂配方Ⅲ（含3％吐温-80、0．5％亚硫酸钠、适量卵磷脂）对双季铵碘均有良好的中和效果，且末见中和剂配方Ⅰ、中和剂配方Ⅲ及其相应中和产物对指示菌金黄色葡萄球菌的生长有明显影响。因此认为，中和剂配方Ⅰ、中和剂配方Ⅲ可分别用于复方二氯异氰尿酸钠和双季铵碘消毒剂消毒效果的鉴定。     

A型流感病毒跨种传播和致病性相关蛋白研究进展

A型流感病毒是重要的人和动物病原体,每年都会在人群中引发季节性流感流行,偶尔还会引发全球流感大流行,对公共卫生安全构成严重威胁.研究发现,A型流感病毒的跨种传播与该病毒的多个蛋白有关,其中血凝素是该病毒宿主范围的主要限制因素,但聚合酶蛋白和非结构蛋白1(NS1)的作用也不容忽视.A型流感病毒通过不断地适应各类宿主和长期...     

Screening and Identification of Protease-producing Bacillus amyloliquefaciens from Pig's Intestines and the Preliminary Study of Its Enzymatic Properties

A Bacillus strains,MY-6 strain which secreted high amount of protease was isolated from healthy pig's intestines,and it was identified as Bacillus amyloliquefaciens by the methods of morphological observation, biochemical tests,16S rDNA sequence amplification and comparison, biolog identification. In order to get an initial understanding of the enzymatic properties of its protease, the dynamic relationship between protease activity and bacterial life cycle, effects of pH,temperature,metal ions and inhibitors on protease activity,and the protease hydrolysis of different substrates were investigated. The results showed that,the protease synthesis of MY-6 strain was synchronized with its spore formation which began in the early stage of the exponential growth phase. The optimum conditions for the protease was 40℃ and pH 7.0,Ca²⁺ and Mn²⁺ had a intensive effects on the protease activity, PMSF could completely inhibit the activity of protease, indicating that the protease was a serine protease.The content of TCA soluble peptides for protein hydrolyzate fish protein, soybean protein, corn protein and casein was 0.83,0.67,0.58 and 0.38 μmol/g,respectively. In conclusion,Bacillus amyloliquefaciens MY-6 strain could be used as an initial selection of probiotic Bacillus to improve protein digestion.