基于调查数据的东海小黄鱼资源变化模式及评价

为筛选表征小黄鱼(Larimichthys polyactis)种群时间变化的主要特征,并分析其变化模式,根据2000―2015年渔业资源调查数据,利用最小/最大自相关因子方法(min/max autocorrelation factors,MAF)将生物学、捕捞强度、空间分布等19个指标进行融合分析,探讨了小黄鱼种群的年际变化模式和资源现状。通过一阶变异值计算各指标与时间的相关性,并以1为时间相关性分组标准,共筛选出7个指标,分别为生殖群体的性比组成(Sexratio S)、纬度分布重心(YCG)、异速生长系数(BS)、铺展面积(SA)、资源密度(CPUES)、条件因子(Condition S)和75%体长。将筛选后的指标用于MAF分析,根据MAF主分量值,两个主分量的一阶滞变异值为0.16、0.19,表现出相对较弱的时间连续性。从MAF得分的位相年际变化上,2000―2015年期间主分量MAF1可划分为3个位相,分别为2000―2002年、2003―2012年、2013―2015年;从趋势变化上,可分为2个趋势,分别为2000―2007年和2010―2015年。主分量MAF2可以划分2个位相,分别为2000―2007年和2010—2014年;从趋势上,可划分为4个趋势,分别为2000―2003年、2004―2006年、2007―2012年和2013—2015年。对第一主分量贡献度最高的指标是生殖群体的纬度分布重心(–0.756)和异速生长系数(–0.609);对第二分量贡献度最高的指标有生殖群体的性比组成(0.590)、异速生长系数(0.539)和扩散面积(–0.606)。主分量MAF1得分的年际变化与纬度分布重心和异速生长系数负相关;MAF2得分与性比、异速生长系数正相关,而与铺展面积负相关。由上可知,MAF1和MAF2在时间尺度上的变化模式反映了各指标的共同变化方向。研究结果为进一步甄选识别种群变化的主要指标及评估资源变化趋势提供科学依据。     

南海北部陆坡金鼻眶灯鱼秋季摄食节律

中层鱼是深海食物网的重要组成部分,其摄食节律的研究对探讨以中层鱼为核心的能量流动与物质循环具有重要意义。本研究利用2014年10月南海北部陆坡海域拖网调查数据,对中层鱼优势种金鼻眶灯鱼(Diaphus chrysorhynchus)秋季的摄食节律进行了研究。结果表明:(1)金鼻眶灯鱼取样体长范围为28~110 mm,集中于50~59 mm体长组,各时间段摄食等级与体长间均不存在正相关;(2)金鼻眶灯鱼在18:00左右上浮摄食,次日05:30左右达到摄食高峰,为连续摄食,5个取样时间段平均摄食等级范围为1.50~2.57,呈增长趋势;(3)差异性分析表明,金鼻眶灯鱼傍晚与夜间或清晨间摄食等级差异性显著,表现在18:30~19:30时间段与01:00~02:00、04:45~05:50或06:30~07:30三个时间段间(P=0.041;P=0.000;P=0.007),另03:30~04:30与04:45~05:50时间段间摄食等级也存在极显著差异(P=0.005);(4)金鼻眶灯鱼各时间段摄食等级频率呈波浪形变化,4级频率在06:30~07:30时间段达到最高峰;0级频率在黄昏18:30~19:30时最高,01:00~02:00降为0,03:30~04:30上升到黄昏时的一半,此后连续下降。总体而言,金鼻眶灯鱼傍晚至次日清晨连续摄食,连续排遗,摄食集中于清晨。综上所述,金鼻眶灯鱼是一种昼夜垂直迁移、夜间连续摄食与排遗的浮游动物食性鱼类,其在南海陆坡水域能量流与物质流中起着承上启下的作用。     

渤海小黄鱼摄食习性

根据2009年8月至2011年5月4个航次渤海底拖网调查所获得的样品,采用胃含物分析法、K-W检验和聚类分析等方法,对38～218 mm体长范围小黄鱼(Larimichthys polyactis)的摄食习性及其随体长、季节的变化进行了研究,并探讨了小黄鱼摄食随海域和年际的时空变化。结果表明:渤海小黄鱼摄食的饵料有40余种,主要以鱼类、虾类和浮游动物为食,优势饵料种类为六丝钝尾虾虎鱼(Amblychaeturichthys heaema)、日本鼓虾(Alpheus japonicus)、太平洋磷虾(Euphausia pacifia)、长额刺糠虾(Acanthomysis longirostris)和中华哲水蚤(Calanus sinicus)。渤海小黄鱼在春夏秋这三个季节中,春季的摄食强度最低,夏季和秋季的摄食强度都较高,不同的是夏季摄食率更高,而秋季的摄食量更高。随着体长的增大,渤海小黄鱼的摄食策略发生了相应的变化。从摄食强度分析,在体长较小时,胃饱满指数低,小黄鱼通过高摄食率来提高摄食强度;随着体长的增加,通过高胃饱满指数来提高摄食强度。从摄食的饵料个体分析,小黄鱼采取了随着体长增加摄食饵料个数减少,饵料个体增大的摄食策略。从摄食的饵料组成分析,小黄鱼随着体长增加发生了显著的食性转换现象:体长小于60 mm属浮游动物食性;体长60～99 mm时,属混合动物食性;体长100～119 mm时,属虾食性;体长超过120 mm,包括了虾/鱼食性和鱼食性。小黄鱼摄食的时空变化与环境中优势饵料生物的数量波动密切相关。     

Blood Chemistry and Histological Properties of Wild and Cultured Sea Bass (<Emphasis Type="Italic">Dicentrarchus labrax</Emphasis>) in the North Adriatic Sea

Histological and biochemical procedures were employed to study liver histomorphology and plasma levels of aspartate aminotransferases (AST), alanine aminotransferase (ALT), triglyceride (TRIG), cholesterol (CHOL), glucose (GLU) and total protein (TP) in cultured (CSB) and wild sea bass (WSB) (Dicentrarchus labrax) from the northern part of the Adriatic Sea. Histopathological changes in liver included varying degrees of infiltration and lipid degeneration of hepatocytes in examined cultured fish. No significant differences between median AST values of CSB (44 IU) and WSB (45 IU) were observed. Values of ALT were <5 IU in both groups. TRIG, CHOL, GLU and TP levels were higher in CSB (2.08 mmol/L, 3.67 mmol/L, 10.66 mmol/L and 49 g/L, respectively) than in WSB (0.67 mmol/L, 2.74 mmol/L, 3.68 mmol/L and 36 g/L, respectively). The study showed differences between plasma biochemical parameters and liver histomorphology of CSB and WSB. This can be explained as a consequence of different diets (artificial in contrast to natural foods), which influence energy metabolism.     

渔业资源可持续利用系统化评价方法的应用研究

渔业资源可持续利用是一个复杂的系统工程，文章以可持续发展的理论为基础，提出了渔业资源可持续利用的发展变，协调度和耦合度的评价方法，该方法具有比传统的生物－经济模型更大的优点，它全面反映了渔业资源可持续利用的各个方面，文章以1978－1990年东海区渔业资源的发展状况作为实证分析，结果表明：1978年以后，东海区渔业资源可持续利用发展和耦合度基本上呈现下降的趋势，而协调度则出现波动的状况。     

Interaction between monk seals Monachus monachus (Hermann, 1779) and marine fish farms in the Turkish Aegean and management of the problem

Mediterranean monk seals Monachus monachus attacked fish on 11 marine fish farms in the Turkish Aegean between 1992 and 2000. There were 40 attacks on fish farms where gilthead sea bream Sparus auratus and European sea bass Dicentrarchus labrax were raised in holding cages. Single seals usually attacked cages at night, regardless of the size of fish in the cages. The seals damaged both holding nets of the cages and fish and, on most occasions, fish escaped as a result of the attacks. With the exception of only one facility, all fish farms attacked by monk seals were concentrated on the large peninsulas including Karaburun and Bodrum Peninsulas, and the number of seal attacks was higher during the winter months. A direct intervention to deter seals such as the use of lights, feeding with pesticide‐injected fish, noise generation, warning and direct shots with rifles and physical exclusion of seals from holding cages by installing antipredator nets were applied to prevent or reduce seal attacks. Our study shows that deterrents such as lights and warning shots were not effective in preventing monk seal attacks. However, both curtain‐ and bag‐type antipredator nets were found to be effective.     

A spatially explicit fitness-based model of capelin migrations the Barents Sea

The geographical distribution and production of the Barents Sea capelin (Mallotus villosus, Osmeridae) is modelled by the use of a state-variable optimization technique (dynamic programming), where the main objective of individuals always is to maximize fitness, or total expected reproduction (R_O), by selecting the most profitable habitats through time. Fitness is gained by successful reproduction (a function of size) during the spawning season on the breeding grounds off northern Norway. The environment (predators, temperature and zooplankton prey) is determined by a meteorologically forced circulation model for the year 1980, creating a spatial and seasonal fluctuation in the environment. Predation from cod is the main source of mortality, and the distribution of the cod (Gadus morhua) stock is assumed to vary with temperature. Growth is predicted from a bioenergetic model, incorporating the cost of swimming between feeding areas and spawning grounds. Field data of the capelin stock recorded during autumn cruises from 1979 is implemented at the start of the model, and then this stock is modelled through 1980 and the first months of 1981. Model predictions are compared with the observed distribution of capelin in autumn 1980. Habitat selection has consequences for the dynamics of the population and growth of individuals, demonstrating the importance of combining external (environmental) and internal (evolutionary) forcing to understand and predict the dynamics of fish populations. This study is the first application of dynamic programming to model the dynamics and ecology of horizontal fish migration, and we suggest that the method may be developed into a useful tool for the management of short-lived species.     

Insights into the divergent evolution of the oceanic squid Sthenoteuthis oualaniensis (Cephalopoda: Ommastrephidae) from the Indian Ocean

Sthenoteuthis oualaniensis is known for its complex population structure with three major transoceanic forms (viz. middle-sized, dwarf, and giant forms) whose taxonomic status has been disputed for decades. This integrated taxonomic study examines these prevenient morphotypes gathered on cruises in the Indian Ocean to ascertain their status in the evolutionary history of the species. Molecular analyses employing mitochondrial (COI, ND2) and nuclear (H3) markers revealed four genetically distinct and novel lineages of the species in the Indian Ocean, representing three morphotypes from the Arabian Sea and one from the Southern Indian Ocean. The mitochondrial-based phylograms revealed two distinct clades in the species: “dwarf forms + giant form” and “middle-sized forms,” which further branch into geographically structured evolutionary units. Species delimitation analyses recovered five distinct clades, namely, the Arabian Sea giant and dwarf forms, Equatorial, Eastern Typical, and Other Middle-sized forms, representing the consensus molecular operational taxonomic units. H3 being heterozygous could not resolve the phylogeny. Haplotype network and AMOVA analysis of mtDNA genes indicated explicit phylogeographic structuring of haplotypes, whereas these outputs and PCA results were incongruent with the morphological grouping. Phenetic features distinguishing the morphotypes were sometimes plastic and mismatched with the genotypes. The giant form was genetically close to the dwarf forms, contradicting the earlier notion that it descended from the middle-sized form. It may be assumed that the dwarf form evolved following sympatric speciation and adaptation to warm equatorial waters, while the focal features of the Western Arabian Sea guide toward allopatric speciation of the giant form.     

Morphologic and cytochemical characteristics of blood cells of juvenile loggerhead sea turtles (Caretta caretta)

A morphologic classification based on the cytochemical characteristics of blood cells of 35 juvenile loggerhead sea turtles (Caretta caretta) is described. Cytochemical stains included benzidine peroxidase, chloroacetate esterase, alpha-naphthyl butyrate esterase (with and without sodium fluoride), acid phosphatase (with and without tartaric acid), Sudan black B, periodic acid-Schiff, and toluidine blue. The morphologic characteristics of erythrocytes were similar to those reported in green turtles. Six types of white blood cells were identified: heterophils, eosinophils, basophils, lymphocytes, monocytes and thrombocytes. Except for the basophils, the rest of the white blood cells from loggerhead turtles had different cytochemical characteristics compared to blood cells from other sea turtle species. The leukocyte differential count was different from that reported for other sea turtle species. Heterophils were the most numerous leukocytes from these loggerhead turtles, followed by lymphocytes, eosinophils, monocytes and basophils. This paper provides a morphologic classification of blood cells of loggerhead sea turtles that is useful for veterinary surgeons involved in sea turtle conservation.     

东海区小黄鱼资源利用现状分析

本文主要通过对东海区小黄鱼近年群体组成变化及其部分生物学特性变化来分析目前该资源利用状况,结果表明：一方面虽然近年小黄鱼产量大幅增加,但其平均体重、平均体长及平均年龄均出现明显下降,资源状况不容乐观;另一方面通过对小黄鱼１９９８年与１９８３年体长体重生长方程比较,发现１９９８年极限体长体重和体重生长速度均小于１９８３年,１龄鱼体长生长速率却大于１９８３年同期生长速度。小黄鱼体垂年龄换算组成表明其一