The effect of breed,sex, and oral meloxicam administration on pain biomarkers following hot-iron branding in Hereford and Angus calves

Hot-iron branding uses thermal injury to permanently identify cattle causing painful tissue damage. The primary objective was to examine the physiological and behavioral effects of oral meloxicam (MEL), compared to a control, administered at the time of hot-iron branding in Angus and Hereford steers and heifers. The secondary objectives were to investigate breed and sex effects on pain biomarkers. A total of 70 yearlings, consisting of 35 heifers and 35 steers (Angus, Hereford, or Angus × Hereford), were enrolled in the study. Animals were blocked by sex, randomized across weight, and assigned to receive MEL (1 mg/kg) or a placebo (CON). Biomarkers were assessed for 48 h after branding and included infrared thermography (IRT), mechanical nociceptive threshold (MNT), accelerometry and a visual analog scale (VAS), and serum cortisol and prostaglandin E₂ metabolites (PGEM). Wound healing was assessed for 12 wk. Hair samples to quantify cortisol levels were taken prior to and 30 d post-branding. Responses were analyzed using repeated measures with calf nested in treatment as a random effect, and treatment, time, treatment by time interaction, breed, and sex as fixed effects. There was a treatment by time interaction for PGEM (P < 0.01) with MEL having lower values than CON at 6, 24, and 48 h (MEL: 18.34 ± 3.52, 19.61 ± 3.48, and 22.24 ± 3.48 pg/mL, respectively; CON: 32.57 ± 3.58, 37.00 ± 3.52, and 33.07 ± 3.48 pg/mL; P < 0.01). MEL showed less of a difference in maximum IRT values between the branded (2.27 ± 0.29 °C) and control site (3.15 ± 0.29 °C; P < 0.01). MEL took fewer lying bouts at 0–12 h (4.91 bouts ± 0.56) compared with CON (6.87 bouts ± 0.55; P < 0.01). Compared with Hereford calves, Angus calves exhibited greater serum but lower hair cortisol, greater PGEM, more lying bouts, and less healed wound scores at 3, 4, and 5 wk. Compared with heifers, steers exhibited lower PGEM, lower branding site and ocular IRT, higher MNT, and lower plasma meloxicam levels. Steers spent more time lying, took more lying bouts and had greater VAS pain, and more healed wound scores at 5 wk than heifers. Meloxicam administration at branding reduced branding and control site temperature differences and reduced lying bouts for the first 12 h. Breed and sex effects were observed across many biomarkers. Changes from baseline values for IRT, MNT, lying time, step count, VAS pain, and wound scoring all support that branding cattle is painful.     

后墙立体栽培草莓提高冬季日光温室内温度

在日光温室的后墙上,采用管道无土栽培方式进行蔬菜或草莓生产,可以提高温室空间利用率和作物种植量,但可能会出现因为管道和植物的挡光而减少后墙蓄热、降低冬季温室温度的问题。为此,通过冬季连续31 d的温度监测,在3种典型气象（晴天、阴天、雪天）条件下,对比分析了有后墙立体基质栽培的日光温室（solar greenhouse with equipment,ESG）和无后墙立体栽培的日光温室（solar greenhouse with no equipment,NSG）温度环境的变化。监测结果表明,ESG的月平均气温较NSG高0.84℃,其中最大日温差为2.22℃,最小日温差为0.14℃。晴天条件下,ESG的日平均冠层温度和1.5 m高度处的空气温度分别是12.72和13.04℃,NSG分别是10.68和11.04℃;ESG的冠层温度最低值是4.68℃,而NSG最低值是4.10℃。可见,ESG较NSG的气温要略高一些;阴天和雪天条件下,2种温室内的温度环境无显著差别。因此,利用日光温室后墙进行立体基质栽培草莓,不但没有降低反而提高了冬季温室内的温度,是一种可行、值得推广应用的温室高效栽培技术。     

马铃薯播种机分体式滑刀开沟器参数优化与试验

针对马铃薯播种机的铧靴式和圆盘式开沟器存在的种薯株距分布不均、播种效果质量差、回土量小等问题,研制出分体式滑刀马铃薯开沟器,并进行关键参数优化,分析开沟和回土过程中土壤颗粒在开沟器上的运动规律,研究种薯落入垄沟后的运动过程,确定影响土壤覆盖种薯效果的因素。采用旋转正交的试验设计方法,以机具前进速度、开沟器侧面夹角、开沟器长度及开沟深度为试验因素,以种薯的横向偏移系数、株距变异系数和回土量为试验指标,分析实施田间试验结果,优化分体式滑刀开沟器的结构参数与最优运动参数:在前进速度为0.95 m/s、开沟器侧面夹角为60°、开沟器长度为500 mm、开沟深度为115 mm时,株距变异系数为10.8%、种薯的横向偏移系数为3.83 mm、回土量为24.1%,满足马铃薯播种作业的要求。该研究结果可为马铃薯开沟播种装置设计与应用提供参考。     

癸甲溴铵对褐牙鲆的急性毒性及抑菌试验

通过药浴方式,进行了癸甲溴铵对褐牙鲆的急性毒性试验;采用二倍稀释法,进行了癸甲溴铵对鳗弧菌、副溶血弧菌的体外抑菌试验;探讨了癸甲溴铵对鳗弧菌引起的褐牙鲆细菌性疾病的防治效果。结果表明,在19.0～21.0℃和24.5～25.5℃时,癸甲溴铵对褐牙鲆的24、48h半数致死浓度(LC50)和安全浓度(Sc)分别为6.35、4.84、0.84和2.43、2.14、0.5mg/L。通过对中毒死亡褐牙鲆的病理组织学观察表明,癸甲溴铵对褐牙鲆的鳃损害最严重,其次是肝脏,对肾脏的损害相对较轻,特别是对鳃的损害与鱼的死亡有很大的关系。在24和32℃,癸甲溴铵对鳗弧菌、副溶血弧菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为10、20和5、10mg/L。防治效果试验表明,癸甲溴铵0.3mg/L浓度药浴可有效防治鳗弧菌引起的褐牙鲆细菌性疾病。     

Celecoxib inhibits viability,induces apoptosis and inhibits autophagy in acute myeloid leukemia cell lines HL-60 and HL-60A

AIM: To investigate the effects of celecoxib on viability, apoptosis and autophagy in acute myeloid leukemia (AML) cell lines HL-60 and HL-60A. METHODS: The HL-60 cells and HL-60A cells were cultured with various concentrations (0, 20, 40, 60, 80 and 100 μmol/L) of celecoxib. The inhibitory effect of celecoxib on the cell viability was evaluated by MTT assay. Apoptosis was analyzed by Annexin-V/PI staining. Apoptosis-related and autophagy-related proteins were determined by Western blot. RESULTS: IC₅₀ of celecoxib were 49.4 μmol/L, 32.0 μmol/L and 25.1 μmol/L for HL-60 cells treated with celecoxib for 24 h, 48 h and 72 h, respectively. For HL-60A cells, the corresponding IC₅₀ were 69.1 μmol/L, 42.5 μmol/L and 29.6 μmol/L, respectively. The results of flow cytometry analysis showed the proportions of Annexin-Ⅴ⁺ PI^-, Annexin-Ⅴ⁺ PI⁺ and Annexin-Ⅴ^-PI⁺ cells were increased in the HL-60 cells, and those of Annexin-Ⅴ⁺PI^- and Annexin-Ⅴ⁺ PI⁺ cells were increased in the HL-60A cells treated with celecoxib for 24 h. After treated with celecoxib, the induction of apoptosis was observed, the apoptosis-related proteins cleaved caspase-3 and cleaved PARP were upregulated, the autophagy-related proteins LC3 II and P62 were both increased, and mTOR, p-mTOR, 4-EBP and p-4-EBP were not changed, indicating that celecoxib inhibited autophagy in the AML cells without the mTOR pathway involvement. CONCLUSION: Celecoxib inhibits the viability of HL-60 cells and HL-60A cells in a time-and dose-dependent manner by its effects of inducing apoptosis and necrosis. Celecoxib inhibits mTOR-independent autophagy in AML cells, indicating a possible way of using celecoxib for enhancing the antitumor activity of therapeutic agents to induce cytoprotective autophagy in the AML cells.     

二次聚类与神经网络结合的日光温室温度二步预测方法

精确预测日光温室温度是实现对温室精准调控的前提。由于温室是复杂非线性系统,受室内外众多环境因素影响,且部分因素难以准确测量和建模,因此,难以通过机理分析建立室外因素精确影响室内温度的物理模型。而现有时间序列分析、人工神经网络等仅基于数据的方法预测准确度也较低。本文提出连续时间段聚类与BP神经网络相结合的二步日光温室温度预测方法。首先,进行二次聚类,对室外温度情况相似的日进行聚类,并将全年划分为若干个类似时间段,根据连续时间段内相似日的数量进行聚类,将全年内的连续时间段归入若干类别。其次,对不同类别的时间段,分别采用BP神经网络建立室外温度、相对湿度、太阳辐射、风速和温室室内温度间的关联模型,通过数据训练,能够较为准确的根据室外环境数据预测室内温度。通过涿州实验农场2年数据试验验证,通过二次聚类,全年连续时间段可划分为3类,通过分别建立BP神经网络并分别训练,结果表明本方法预测误差仅为6.23%,与现有未分类的BP神经网络预测算法对比,本文方法有效地提高了准确度,平均误差降低5.4个百分点。     

Ethanol extract from Cortex Albiziae attenuates mouse acute liver injury induced by carbon tetrachloride via modulation of NF-κB pathway

AIM:To investigate the protective effect of ethanol extract from Cortex Albiziae on acute liver injury, and to explore its possible mechanism. METHODS:Acute liver injury in mice was induced by single intraperitoneal injection of 25% carbon tetrachloride (olive oil solubilization). The effective parts of ethanol extract from Cortex Albizziae against acute liver injury were screened. The pathological changes of the liver tissues were examined by pathological sections with HE staining. The activity of total superoxide dismutase (T-SOD) and the content of malondialdehyde (MDA) of the liver tissues were detected, the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were mea-sured by ELISA, and the protein expression levels of NF-κB p65, Bcl-2 and Bax in the liver cells of the mice in each group were determined by Western blot. RESULTS:Compared with model group, the serum levels of AST and ALT in low-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-L, 4 mg·kg^-1·d^-1) group and high-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-H, 8 mg·kg^-1·d^-1) group were significantly decreased. The necrosis extent and degree of the hepatocytes and infiltration of inflammatory cells were significantly lower than that in model group. Compared with model group, the serum levels of TNF-α and IL-6 in AB-H group and AB-L group were significantly decreased (P<0.05). The protein level of NF-κB p65 in the nuclei of mouse liver cells in AB-H group and AB-L group were also decreased significantly (P<0.05). Compared with model group, the protein expression of Bax was decreased, the protein expression of Bcl-2 was increased, and the Bcl-2/Bax ratio was increased in AB-L group and AB-H group. CONCLUSION:The n-butanol phase of ethanol extract from Cortex Albiziae may protect the liver by reducing the activation of NF-κB p65, inhibiting the excessive release of inflammatory cytokines IL-6 and TNF-α, and decreasing hepatocyte apoptosis via regulating Bcl-2 and Bax expression.     

基于USB Key的水产品企业监管溯源系统设计与应用

根据目前水产品监管溯源系统中水产品企业身份认证存在的问题,设计了一种基于USB Key的水产品企业监管溯源系统。首先,分析了几种身份认证技术的方法,重点阐述了其优缺点;然后,设计了USB Key硬件电路关键部分、软件系统以及基于USB Key认证的水产品企业的认证流程,解决了水产品信息传输的安全性问题;最后,描述了基于USB Key的水产品企业监管模式在江苏省水产品企业认证与监管追溯平台中的应用。研究结果表明,该监管溯源系统便捷安全,稳定可靠,能实现产地准出、市场准入、企业资质认证、政府实时监控的水产品企业监管溯源体系。     

3FDD―6型后悬挂滚筒式棉花打顶机

棉花适时打顶心是棉花生产过程中的关键环节。本文简要介绍了一种新型棉花打顶机具—3FDD-6型后悬挂滚筒式棉花打顶机的主要结构、工作原理、主要技术参数。并对主要结构部件进行了设计介绍,同时也分析了该机的社会效益与经济效益。     

视觉导引AGV鲁棒特征识别与精确路径跟踪研究

针对AGV多分支路径与工位点标识的可靠识别以及导引路径的精确跟踪问题,提出了一种基于双视野窗口的鲁棒特征识别与精确路径跟踪方法。采用整幅视野范围作为模式识别窗口,在该窗口采用基于核主成分分析(KPCA)和BP神经网络的识别方法,将路径特征通过核函数映射到高维空间进行PCA降维,再利用BP神经网络识别降维后的样本矩阵。同时提出一种导引扫描窗口设置方法,该窗口范围取决于摄像机竖直视角以及摄像机安装倾斜角,在导引扫描窗口内将导引路径简化为直线模型并用最小二乘法拟合,针对拟合直线计算导引所需的路径偏差。实验结果表明,KPCA-BP方法显著提高了路径特征识别的实时性和鲁棒性,6类路径特征的平均特征识别正确率为99.5%;导引扫描窗口有效减小了导引路径直线拟合的计算误差,直线路径跟踪误差小于3 mm,曲线路径跟踪误差小于30 mm。