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81.
【目的】探讨钙信号对小鼠脂肪组织中GPR120基因转录及脂肪生成的影响。【方法】用外源性葡萄糖酸钙和二氢吡啶钙离子通道的阻滞剂Nifedipine处理小鼠30 d,记录小鼠体质量的变化,测定小鼠皮下脂肪、附睾脂肪和肾周脂肪的沉积量,检测血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)的浓度,同时利用Real-ti me PCR法分析GPR120基因及与脂肪生成密切相关的转录因子PPARγ、C/EBPα和脂解基因HSL、生脂基因FASmRNA的表达情况,用SPSS软件分析小鼠附睾脂肪中GPR120与脂代谢相关基因表达的相关性。【结果】葡萄糖酸钙可延缓小鼠体质量的增加,减少体脂含量(P0.01),并使小鼠血清中的TG、TC和LDL-C浓度极显著降低(P0.01),HDL-C浓度显著升高(P0.05),可导致GPR120、PPARγ、C/EBPα和FAS的mRNA表达水平降低,且分别达到极显著(P0.01)或显著(P0.05)水平,并可使HSLmRNA表达水平极显著升高(P0.01);Nifedipine处理能使小鼠体质量增加(P0.05),促进体脂沉积(P0.01),并使小鼠血清中的TG和TC浓度显著升高(分别为P0.05和P0.01),GPR120、PPARγ、C/EBPα和FAS的mRNA表达水平极显著升高(P0.01),但却使HSLmRNA的表达水平极显著降低(P0.01)。相关性分析表明,GPR120 mRNA的表达水平与PPARγ、FAS和C/EBPα间呈显著正相关。【结论】钙信号在GPR120调节小鼠脂肪生成的过程中可能起到负调控作用。 相似文献
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分别用25%阿密西达SC、2%菌克毒克AS、4%农抗120AS防治平贝母锈病,与化学农药粉锈宁对比试验。结果表明:阿密西达防效为90.1%;菌克毒克防效为88.1%;农抗120防效为85.2%,粉锈宁防效为74.7%.生物农药防治效果比化学农药提高10.5-15.7个百分点,与对照比较均达到差异显著、极显著水平。 相似文献
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A spinosad-based fruit fly bait, GF-120, has recently become a primary tool for area-wide suppression or eradication of pest tephritid fruit flies. The present study assessed the attraction and feeding of five non-target fly species to GF-120 in Hawaii. These non-target flies include three beneficial tephritid species [Eutreta xanthochaeta (Aldrich), Tetreuaresta obscuriventris (Loew), Ensina sonchi (L.)] introduced for weed biological control, an endemic Hawaiian tephritid [Trupanea dubautiae (Bryan)] (all Diptera: Tephritidae) and the cosmopolitan Drosophila melanogaster Meigen (Diptera: Drosophilidae). All five non-target fly species were susceptible to GF-120, as was the target pest Mediterranean fruit fly Ceratitis capitata (Wiedemann). Feeding on, or even brief tasting of, GF-120 killed all fly species within 2 h. When individual flies were provided with a choice of GF-120 or honey solution, there was no difference in the frequency of first food encounter by E. xanthochaeta, D. melanogaster or C. capitata. The other three non-target species approached honey more often than GF-120 in their first food encounter. Feeding times on GF-120 and honey were not significantly different for D. melanogaster and C. capitata, while the other four non-target species fed longer on honey than on GF-120. There was no significant difference in feeding time on honey versus GF-120 between males and females of each species. These results suggest that area-wide treatment using GF-120 for the purpose of eradication of pest fruit flies has potential negative impacts on these and other non-target fly species in Hawaii. 相似文献
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脂肪酸在动物脂质代谢和免疫调节中起着至关重要的作用,G蛋白偶联受体120(G-protein coupled receptor 120,GPR120)又被称为游离脂肪酸受体4(free fatty acid receptor 4,FFAR4)是G蛋白偶联受体家族成员。它可以被长链脂肪酸激活,参与调节机体糖脂代谢,是治疗人及畜禽脂质代谢紊乱及免疫调节等相关疾病的热点靶点。本文就近来GPR120在脂肪组织中的研究现状、对免疫调节的作用及其激动剂或配体作为药物运用的可能做一综述。 相似文献
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1987~1989年进行了农抗120与常用化学杀菌剂防治西瓜枯萎病的药效对比,及不同使用期农抗120的防病效果试验,结果表明,全省多点用300倍农抗120,施药2次,防治西瓜枯萎病的效果平均为83.91±11.86%,比常用化学杀菌剂高23~43%,农抗120的防效与施药时期关系密切,两次灌根时间都应在发病之前,第一次施药时间:直播西瓜在4~5片真叶期,移栽西瓜在苗床期,右,据河南省120个县市,11万亩西瓜的防治示范结果,隔7~10天,进行第二次施药,病株率已控制在0~8%左平均防效为70~95%,每亩挽回西瓜损失200~400公斤。 相似文献
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Giordano A Rossi G Pieralisi C Paltrinieri S 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2008,37(1):21-30
BACKGROUND: The ADVIA 120 is an automated laser cell counter widely used in veterinary medicine. Although specific software for equine samples is available and validated, only a few reports have been published comparing the ADVIA 120 with other methods for equine hemogram evaluation. OBJECTIVES: The purpose of this study was to compare the hematologic values and reference intervals obtained on the ADVIA 120 with those obtained on an impedance cell counter and manual differential counts in healthy horses. METHODS: EDTA-anticoagulated blood samples were obtained from 114 clinically healthy horses of various breeds, both sexes, and 2-6 years of age. Samples were stored for up to 12 hours at 4 degrees C and then analyzed on the ADVIA 120 and the Hemat 8. A 100-cell to 200-cell differential leukocyte count was performed by 3 independent observers on May-Grünwald-Giemsa-stained smears. Intra-assay precision of the ADVIA 120 was determined by analyzing 5 replicates each of 10 of the blood samples. RESULTS: Results from the ADVIA were significantly higher than those from the impedance counter for RBC count, total WBC count, hemoglobin concentration, red cell distribution width, MCH, and MCHC, and significantly lower for HCT and platelet count. Significantly higher neutrophil and basophil counts and significantly lower lymphocyte counts were obtained with the ADVIA 120 compared with manual counts. Based on Passing-Bablok regression analysis, RBC and platelet counts were in good agreement between the 2 analyzers; a constant and proportional bias was present for other values. Coefficients of variation for erythrocyte parameters on the ADVIA were <1%, but were higher for platelet (6%), total WBC (2%), differential WBC (4%-30%), and reticulocyte (75%) counts. CONCLUSIONS: Results obtained with equine samples on the ADVIA 120 were comparable with those obtained on an impedance counter; reference intervals differed statistically but overlapped. The ADVIA had poor precision for reticulocyte and differential leukocyte counts such that the latter should always be verified on smears. 相似文献
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