~(60)Coγ射线辐射小麦种子贮藏效应的研究

用 2 5 0、30 0、35 0和 40 0Gy 4种不同剂量的60 Coγ射线照射鄂恩 4号小麦干种子 ,测定了照射后及时播种和贮藏 1 5 0d播种对小麦的发芽势、发芽率、幼苗高、幼苗根数和根长的影响。结果表明 :被照射的小麦种子 ,贮藏 1 5 0d后 ,较及时播种的小麦种子其发芽和生长得到了一定程度的恢复 ,即贮藏减轻了辐射损伤 ,但辐射效应仍然存在。     

~（60）Co γ射线不同剂量辐射甘蔗种芽试验初报

采用２０００γ、３５００γ、５０００γ、６５００γ、８０００γ５个等级的６０Ｃｏγ射线对甘蔗种芽进行电离辐射处理，观察对甘蔗生长发育的影响及苗期辐射形态变化，发现随着辐射剂量的增加出苗率明显下降，生长亦严重受阻，并发生矮化苗，茎色改变，心叶不抽出，株型变化等多种辐射形态变化。３５００γ以上的处理总变化数均在５０％以上，尤以５０００γ以上处理总变化率高，但差异不显著。初步认为蔗芽辐射育种的剂量范围为３５００－８０００γ，尤以３５００－６５００γ１为宜。     

60Co辐照装置增源后剂量场分布及剂量测定

为使60Co辐照装置增加总活度为28878居里(1．07PBq)由7根活度不同的进口源棒的增源顺利启用,通过测定源到辐照位置的重复性为0．14％<1％,符合标准。测定从铁皮盖板到源中心的高度为90．3cm。五次升源所得剂量率(Gy/min)分别为:11．4、10．95、10．98、10．89、10．87,相对偏差为0．55％<1％．说明辐射场空间剂量率精确性高。计算辐照场空间分布的不均匀度U0=Dmax／Dmin=1．46(U0<1.5),表明在同一参考面上,辐射源剂量场剂量分布符合要求。     

Electrophoretic comparison of the genomes of North American bluetongue viruses, one Australian bluetongue virus, and three other related orbiviruses

The genomes of U.S. bluetongue viruses, an Australian bluetongue virus, and three other related orbiviruses were analyzed by polyacrylamide gel electrophoresis. The genomes were comprised of ten segments of double-stranded (ds) RNA. Estimates of the molecular weights of the dsRNA segments revealed that the U.S. bluetongue serotypes were remarkably similar. Although the dsRNA profiles of the viruses exhibited common segments, each virus had a distinct dsRNA profile. The usefulness of the genome analysis as a diagnostic tool for identification and for epidemiologic studies is discussed.     

γ-聚谷氨酸高产菌株的鉴定及诱变选育

从豆豉中分离筛选出一株产γ-聚谷氨酸（γ-PGA）的菌株,经过形态、生理生化鉴定及16SrRNA基因序列分析,确定为枯草芽孢杆菌。其中Bacillus subtilis HD11γ-PGA产率最高,达6.8g/L。以HD11作为出发菌株,采用紫外线、硫酸二乙酯和亚硝基胍对其进行复合诱变,分离筛选得到一株稳定的高产突变株Bacillus subtilisHD-F9,经摇瓶发酵γ-聚谷氨酸的含量达到10.72g/L,较出发菌株提高56.3%。     

不同加成数C_(60)-β-丙氨酸衍生物的还原能力及金属螯合能力

为研究水溶性富勒烯衍生物抗氧化性能,通过控制反应配比,合成三种具有不同加成数目的水溶性C60-β-丙氨酸衍生物,C6(0NHCH2CH2COONa)nHn。采用红外、紫外、核磁共振、元素分析等手段对其进行结构表征。元素分析表明,三种不同加成数目的水溶性C60-β-丙氨酸衍生物A、B、C的加成数分别为2、4、8。同时考察三种衍生物的还原能力及金属螯合能力。结果表明,在还原能力体系中,当三种衍生物A、B、C浓度为2.5μmol.mL-1时,吸光度分别为0.424、0.612和1.040;在金属螯合能力体系中,三种衍生物A、B、C的半螯合浓度(当螯合率达到50%时,所需螯合物的浓度)分别为0.348、0.261和0.135μmol.mL-1。随着加成数目的增多,C60-β-丙氨酸衍生物的还原能力及金属螯合能力增强。     

Experiment Study on Soil Properties of Shennv Stream Landslide in Wu Gorge

Accurate obtainting soil parameters of landslide is the key links for landslide stability evaluation and project management . This article takes Shennv Stream landslide in Chongqing Wushan County as an example, implementing unconsolidated and undrained shear test and mineral composition analysis by X ray diffraction for soil in different elevations of landslide ,conducting sensitivity test for various soil parameters of reservoir landslide change with different water level.Study shows that : Mineral composition of soil mainly contains Calcite, Quartz and clay mineral. Hydrophilic minerals in clay mineral have great effect on stability of landslide. Shear strength decreases by influence of water content on cohesion. Cohesion decreases sharply in a quadratic function relation with water content increasing .When water content comes to 18.21% ,change of cohesion tends to be stable.But the internal friction angle of landslide soil decreases linearly in small amplitude with an increase of water content. Research results may provide some important references for rationally choosing strength parameters of soil in similar landslide.     

Obesity increases initial rate of fibrin formation during blood coagulation in domestic shorthaired cats

Obesity predisposes to a prothrombotic state in humans, but whether a similar state occurs in obese animals is unknown. The objective of the current study was to examine the effect of body fat percentage (BF) on haemostatic parameters including thromboelastography with tissue factor as activator (TF-TEG) in client owned indoor-confined physically inactive cats. Seventy-two cats were included following an initial thorough health examination, and a complete blood count, biochemistry panel, conventional coagulation panel and a TF-TEG analysis were performed with tissue factor (1:50,000) as activator. The cats were anaesthetized, and BF was measured using Dual-energy X-ray absorptiometry. Significant difference between lean (BF < 35%, n = 26), overweight (35% < BF < 45%, n = 28) and obese (BF > 45%, n = 18) cats was identified using ANOVA. The correlation between BF, serum leptin and total adiponectin, respectively, with individual TEG and conventional coagulation parameters was evaluated. Obese cats showed a faster rate of fibrin formation (TF-TEG(R), p < 0.05), and TF-TEG(R) was positively correlated with plasma leptin levels. Increasing BF did not affect other conventional coagulation or TF-TEG parameters. In conclusion, this study indicates a connection between body fat content and altered haemostasis, also in cats. Whether feline obesity causes a hypercoagulable state of clinical relevance should be further investigated.     

Protective role of 14-3-3γ in burn and LPS-induced rat myocardial injury

AIM: To study the protective role of 14-3-3γ in burn or LPS-induced myocardial injury. METHODS: The rat model of burn or LPS-induced injury was established. The heart functions and 14-3-3γ protein expression were detected 3 h, 6 h, 12 h and 24 h after treatment. Primary neonatal rat cardiomyocytes were used in vitro. pFLAG-14-3-3γ plasmid was constructed and transfected into the cardiomyocytes 24 h before LPS-induced injury. The injury in the cardiomyocytes was evaluated by measuring the cell viability and the level of lactate dehydrogenase(LDH). Apoptosis of cardiomyocytes was detected by flow cytometry. Opening of mitochondrial permeability transition pore (mPTP) was also determined by Ca²⁺-induced swelling of isolated myocardial mitochondria. RESULTS: The expression of 14-3-3γ was elevated following the burn or LPS-induced myocardial injury in vivo. In vitro, transfection with pFLAG-14-3-3γ plasmid in to the cardiomyocytes significantly protected against LPS-induced injury. Compared with the cardiomyocytes without transfection with pFLAG-14-3-3γ plasmid, higher cell viability rate and lower LDH release, cell apoptosis and mPTP opening were observed in the cardiomyocytes transfected with pFLAG-14-3-3γ plasmid. CONCLUSION: The 14-3-3γ protein protects the heart against burn or LPS-induced injury by inhibiting the mPTP opening.     

微小隐孢子虫CP15-P23-CP15/60基因的融合表达及抗血清的制备

以微小隐孢子虫基因组DNA为模板,PCR扩增获得子孢子表面抗原CP15、P23和CP15/60基因。利用重叠延伸PCR(SOE PCR)将该3段基因片段串联在一起,各基因片段之间引入柔性氨基酸接头(GGGGS)编码基因。将串联基因克隆到原核表达载体pET-28a(+)上,构建重组表达载体,转化到大肠埃希菌BL21(DE3)中进行诱导表达,将纯化的重组蛋白免疫Balb/c小鼠制备多克隆抗体。结果表明,获得了CP15-P23-CP15/60融合基因,并在大肠埃希菌中高效表达,Western blot显示重组蛋白能被牛抗微小隐孢子虫阳性血清识别,制备的多克隆抗体能被重组蛋白特异性识别,表明获得的重组蛋白具有较好的抗原性。