鹅源H5N1亚型禽流感病毒感染SPF雏鸡免疫球蛋白含量的变化研究

以7日龄SPF雏鸡为试验动物,采用常规间接酶联免疫吸附试验(IELISA)法,对鹅源H5N1亚型禽流感病毒(AIV)感染SPF雏鸡后,其泪液、气管液、肠液、胆汁的IgA、IgG、IgM含量的动态变化进行了检测.结果发现,鹅源H5N1亚型中强毒AIV感染SPF雏鸡后,其泪液、气管液、肠液、胆汁的免疫球蛋白IgA、IgM含量均在1～3 d或1～4 d显著低于相应对照雏鸡(P＜0.05),而感染后第5天,上述被检指标开始升高,并于5～8 d或5～14 d,病毒感染雏鸡的IgA和IgM显著高于对照雏鸡(P＜0.05或P＜0.01).表明7日龄SPF雏鸡感染AIV后的早期,其消化道和呼吸道的体液免疫功能降低.     

如何提高犊牛成活率

犊牛是高产奶牛的第一步。同时犊牛饲养存在很高风险。尤其在断奶前的饲喂。如何降低发病率提高成活率是每个牧场都在努力的目标。现将犊牛饲养控制点整理汇报,希望能给大家一定的参考。     

浅述母猪的安全分娩

母猪的分娩包括分娩前的准备、接产与助产、产后护理3个环节,母猪产仔成活数的多少直接关系到养猪的经济效益,所以应保证母猪安全分娩。根据笔者多年的生产实践,现将母猪的安全分娩浅述如下。     

一例瘤胃切开术诊断性治疗奶牛瘤胃异物

某养殖户一头荷斯坦奶牛出现食欲不振、采食量和产奶量下降、精神萎靡等症状,疑似为瘤胃异物继发前胃弛缓,间歇性瘤胃鼓胀,决定采取瘤胃切开探查术进一步确诊。最终在网胃底部掏出约200g铁渣和机器螺丝,并在瘤胃底部及网瓣孔处掏出缠成团状并已发生部分钙化的绳子和地膜,术后该奶牛恢复健康。     

浅谈蚕期后“回山消毒”的几种做法

养蚕期结束后,往往都是夏收或者秋忙季节,广大蚕农往往忽视蚕期后的＂回山消毒＂工作,即使做了也是马马虎虎,不能有效降低病毒,易造成大环境污染。事实上,＂回山消毒＂是下期蚕前消毒的基础,所以必须做到超前准备、工作到位、不能仓促。经过一季蚕的连续饲养,上蔟采茧后,蚕室中病原物及病死蚕体的病原数量最多、病原的毒力最强,所以要及时杀菌消毒。     

家兔腹泻病的防治

家兔腹泻病是指临床上具有腹泻症状的一类疾病,主要表现为粪便不成球形,稀软,呈粥状或水样。各个年龄段的家兔均可发生,以断奶前后的幼兔发病率最高,治疗不当常引起死亡。该病各地饲养场(户)均有发生,特别是1~3月龄的仔兔,死亡率轻者在5%~10%,严重者可达100%。该病病因多,如饲料不清洁,混有泥沙、污物等或饲料发     

Research on the Antioxident Function of Sulforaphane on the Leydig Cells of Cadmium Exposured Mice

The study was aimed to explore the antioxident function of sulforaphane (SFN) on the leydig cells of cadmium (Cd) exposured mice.Cd and SFN were added to the cell culture medium of TM3 cell, half inhibitory concentration (IC₅₀) of Cd and SFN safe dose range were determined.The tested model was set up,and the relative survival rate of TM3 cells, lactate dehydrogenase (LDH) activity and cell antioxidant levels were determined to study the antioxident function of SFN to cadmium exposured mice.The results showed that:①With the increase of Cd concentration,the relative survival rate of TM3 cells was decreased,and the IC₅₀ of Cd was 51.4 μmol/L;Within a certain concentration range,SFN could increased the cells survive rate,but there was toxicity when the SFN concentration was more than the scope,and the greater the concentration,the greater the toxicity. At experimental condition,SFN safety concentration were 2.5,5 and 10 μmol/L.②Compared with the control group,the GSH content,the T-SOD and GSH-Px activities in Cd group were significantly or extremely significantly decreased (P < 0.05;P < 0.01),and the LDH activity,the MDA content were increased.Additionally,the LDH activity and MDA content of SFN groups were decreased,while others three indexes were increased. Compared with Cd group,the GSH content,the T-SOD and GSH-Px activities of Cd+SFN groups were significantly or extremely significantly increased (P < 0.05;P < 0.01),however,the LDH activity,MDA content were decreased. The result indicated that SFN had the antagonism effect on toxicity of Cd in TM3 cell.     

牦牛成纤维细胞敲低HO-1基因后对相关基因表达的影响

血红素加氧酶1(heme oxygenase1,HO-1),是血红素分解代谢过程中的限速酶,起到重要的抗氧化损伤作用,HO-1活性的改变直接影响抗氧化损伤能力的变化。为研究HO-1基因在牦牛高海拔低氧适应的氧化损伤功能,通过构建HO-1基因的siRNA序列,转染牦牛胎儿成纤维细胞,试验前期发现HO-1基因过表达后与CYGB(胞红蛋白)、eNOS(内皮型一氧化氮合酶)基因相关。通过qRT-PCR和蛋白免疫印记(Western blot)检测转染HO-1 siRNA序列的牦牛成纤维细胞在正常条件和低氧条件(5%氧气)下0,24,48,72 h相关基因和蛋白表达变化。结果显示:siRNA转染牦牛成纤维细胞后,试验组HO-1的表达量显著低于空白组和阴性组(P0.01),试验组CYGB表达量也低于空白组和阴性组(P0.01),且HO-1、CYGB蛋白表达量在72 h最低,试验组eNOS mRNA表达量显著低于空白组和阴性组,eNOS蛋白未见表达。结果揭示:牦牛成纤维细胞敲低HO-1基因后,HO-1基因和蛋白表达量明显下调,且对携氧蛋白CYGB及eNOS信号通路的表达具有相同的影响。该结果为牦牛HO-1的功能及其在牦牛缺氧保护机制的研究提供理论依据。     

基于Fiber1基因分型的安卡拉病毒感染病例实验室检测

为确诊贵州省某养鸡场发生疑似安卡拉病毒感染的病例,实验基于安卡拉病毒Fiber1基因设计合成引物,对病料样本进行病原核酸检测和核酸测序与序列分析。结果:(1)从病例的肝脏样本中扩增出大小约1 296 bp的Fiber1基因目的片段,与预期结果相符。(2)序列测定与分析显示,扩增的Fiber1基因序列与国内分离株序列同源性高,亲缘关系近。(3)系统进化分析显示,感染病例中的安卡拉病毒流行株(命名为:GZ-LPS)与K31、MX-SHP9、ON1和河北、江苏等地的FAdV-4分离株处于同一进化分支,而与其他血清型禽腺病毒株处于不同进化分支。结论:此次疫情经实验室诊断,确定为禽腺病毒Ⅰ群C种血清4型的安卡拉病毒感染所致。