草鱼SGLT1/2基因克隆及葡萄糖处理对其mRNA表达的影响

作为重要的转运载体,SGLTs(钠依赖性葡萄糖协同转运蛋白,sodium-glucose co-transporters)在物质的吸收过程中发挥着关键的作用.SGLT1和SGLT2作为SGLTs家族的重要成员,参与调节体内的葡萄糖吸收,从而维持血糖的稳态.为研究SGLT1和SGLT2在草鱼葡萄糖吸收过程中的作用,本实验...     

基于 ArcGIS Server 9.0和 J2EE 构建多层结构的森林资源 WebGIS

阐述了基于ArcGIS Server 9.0和J2EE技术的多层结构森林资源WebGIS的开发思想和各功能层的实现技术,在开发过程中以"瘦客户"模式作为本系统的结构模式,并采用MVC设计模式对系统功能进行分割。同时,以大兴安岭塔河林业局森林资源数据为例,介绍了森林资源空间数据的发布、森林资源WebGIS系统的组成以及其界面的定制、主要功能等。结果表明:ArcGIS Server 9.0、J2EE技术与MVC设计模式的综合使用,使整个森林资源WebGIS系统的开发效率、数据安全性、跨平台可用性、系统功能可扩充性和使用灵活性等方面较传统的双层体系结构系统得到了较大的优化和提升。     

通过核基因ITS2片段研究四种鲇形目鱼类进化

采用鲇形目鱼鱼类通用引物扩增了鲇形目鱼类4种鱼,长吻(Leiocassi longirostris)、南方南方大口鲇(Si-lurus meridionalis)、黄颡鱼(Pseudobagrus fulvidraco)、斑点叉尾(Ictalurus punctatus)核DNA的ITS2片段,并构建UPMGA,NJ,ME和MP系统树。结果显示:根据遗传距离,斑点叉尾和黄颡鱼的亲缘关系最近(D=0.0661),接下来是黄颡鱼和南方大口鲇(D=0.1100),南方大口鲇和长吻(D=0.1184),斑点叉尾和南方大口鲇(D=0.1266),黄颡鱼和长吻(D=0.1490),斑点叉尾和长吻的亲缘关系是最远的(D=0.1503)。结果表明:长吻最早分化,其次是南方南方大口鲇和黄颡鱼,而斑点叉尾分化最晚。     

猪皮炎肾病综合征组织病理学和PCR诊断

应用病理学和PCR方法,对浙江某猪场送检的疑似猪皮炎肾病综合征（PDNS）病猪进行诊断。结果发现肾脏呈现纤维蛋白性肾小球肾炎变化,真皮及皮下血管表现为坏死性脉管炎,淋巴组织中大量淋巴细胞缺失、多核巨细胞浸润,PCR扩增产物显示猪圆环病毒2型（PCV-2）阳性。结果表明,该猪场感染了PCV-2,并表现为PDNS的病理特征。     

环境因素对淡水浮丝藻生长及产生嗅味的影响

研究温度、营养盐浓度以及光照强度对淡水浮丝藻(Planktothrix sp.)及其代谢产物二-甲基异莰醇(2-MIB)的影响,为控制丝状蓝藻生长及预警水体嗅味物质污染提供实践依据。浮丝藻由中国科学院水生生物研究所淡水藻种库分离于安徽巢湖;设置的实验温度为15、20、25、30℃,BG-11营养盐溶液体积分数为20%、30%、50%、100%,光照为1 000、2 000、3 000、4 000 lx。结果表明,温度对浮丝藻生长密度、总2-MIB生成量及胞外2-MIB分泌量影响最显著,25℃是浮丝藻最适宜生长温度,最高细胞密度可达6.04×10⁵个/mL,30℃是其最适宜的产2-MIB温度,总2-MIB生成量和胞外2-MIB分泌量均最高,分别为3.64×10³ng/L和2.78×10³ng/L;其次为营养盐浓度,增大BG-11营养盐浓度,浮丝藻生长密度和总2-MIB生成量会随之增加,低浓度营养盐时胞外2-MIB分泌量所占比例较高,表明低浓度营养盐更有利于浮丝藻进行胞外2-MIB分泌;光照对浮丝藻生长及产嗅味物质影响较小,...     

β-Carotene from the Alga Dunaliella bardawil Decreases Gene Expression of Adipose Tissue Macrophage Recruitment Markers and Plasma Lipid Concentrations in Mice Fed a High-Fat Diet

Vitamin A and provitamin A carotenoids are involved in the regulation of adipose tissue metabolism and inflammation. We examined the effect of dietary supplementation using all-trans and 9-cis β-carotene-rich Dunaliella bardawil alga as the sole source of vitamin A on obesity-associated comorbidities and adipose tissue dysfunction in a diet-induced obesity mouse model. Three-week-old male mice (C57BL/6) were randomly allocated into two groups and fed a high-fat, vitamin A-deficient diet supplemented with either vitamin A (HFD) or β-carotene (BC) (HFD-BC). Vitamin A levels in the liver, WATs, and BAT of the HFD-BC group were 1.5–2.4-fold higher than of the HFD group. BC concentrations were 5–6-fold greater in BAT compared to WAT in the HFD-BC group. The eWAT mRNA levels of the Mcp-1 and Cd68 were 1.6- and 2.1-fold lower, respectively, and the plasma cholesterol and triglyceride concentrations were 30% and 28% lower in the HFD-BC group compared with the HFD group. Dietary BC can be the exclusive vitamin A source in mice fed a high-fat diet, as shown by the vitamin A concentration in the plasma and tissues. Feeding BC rather than vitamin A reduces adipose tissue macrophage recruitment markers and plasma lipid concentrations.     

硅对盐胁迫下黄瓜幼苗根系质膜、液泡膜酶活性的影响

研究硅对盐胁迫下黄瓜幼苗生长及根细胞质膜H^＋-ATPase、液泡膜H^＋-ATPase、H^＋-PPase和Ca^2＋-ATPase活性的影响。结果表明：盐胁迫严重抑制黄瓜幼苗生长,根细胞质膜H^＋-ATPase、液泡膜H^＋-ATPase、H^＋-PPase和Ca^2＋-ATPase活性明显降低。硅处理明显提高盐胁迫黄瓜根液泡膜H^＋-ATPase、H^＋-PPase、Ca^2＋-ATPase活性,一定程度上维持了液泡膜质子泵活性,有效地防御了细胞质酸化,这可能是硅提高黄瓜耐盐性的一个重要因素。     

保护地CO2施肥研究进展

对保护地内ＣＯ的变化规律,ＣＯ２施肥的效果,增产的原因,施肥时间等的研究结果,进行了综述,并介绍了ＣＯ２的施用方法、影响施肥效果的因素及注意事项。     

Molecular evidence for cosmopolitan distribution of platyhelminth parasites of tunas (Thunnus spp.)

Global distribution of platyhelminth parasites and their host specificities are not well known. Our hypothesis was that platyhelminth parasites of large pelagic fishes are common around the world. We analysed molecular variation in three different taxa of platyhelminth parasites infecting four species of tunas: yellowfin tuna (Thunnus albacares, Scombridae) from Western Australia, southern bluefin tuna (Thunnus maccoyii, Scombridae) from South Australia, Pacific bluefin tuna (Thunnus orientalis, Scombridae) from Pacific Mexico and northern bluefin tuna (T. thynnus, Scombridae) from two localities in the Mediterranean (Spain and Croatia). Comparisons of ITS2 and partial 28S rDNA demonstrated two congeneric species of blood flukes (Digenea: Sanguinicolidae) from multiple hosts and localities: Cardicola forsteri from southern bluefin and northern bluefin tunas, and Cardicola sp. from Pacific bluefin and northern bluefin tunas; and a gill fluke, Hexostoma thynni (Polyopisthocotylea: Hexostomatidae), from yellowfin, southern bluefin and northern bluefin tunas. Partial 28S rDNA indicates that a second type of fluke on the gills, Capsala sp. (Monopisthocotylea: Capsalidae), occurs on both southern bluefin and Pacific bluefin tunas. This appears to be the first report of conspecific platyhelminth parasites of teleosts with a wide‐ranging geographical distribution that has been confirmed through molecular approaches. Given the brevity of the free‐living larval stage of both taxa of flukes on the gills (H. thynni and Capsala sp.), we conclude that the only feasible hypothesis for the cosmopolitan distribution of these flatworms is migrations of host tunas. Host migration also seems likely to be responsible for the widespread occurrence of the two species of blood flukes (Cardicola spp.), although it is also possible that these were translocated recently by the spread of infected intermediate hosts.     

辣椒新品种川腾2号的选育

川腾2号辣椒是以自交系03-8-4-2作母本,以自交系03-9-4-6作父本配制而成的一代杂种。该品种极早熟;果实粗牛角形,纵径12.2cm,横径4.5cm,果肉厚0.4cm,单果质量53.2g;青熟果深绿色,老熟果红色,外观商品性好;肉质脆嫩,微辣,适鲜食。露地667m2产鲜椒3000kg左右。耐寒、耐热、耐旱、耐涝,抗病毒病、疫病、炭疽病、蚜虫、跗线螨、烟青虫等的能力超过湘研1号,与湘研11号相当,为强或较强,适合四川和全国种植。