中、美学校体育课程设置比较分析*

运用比较分析等方法，对宾夕法尼亚州和上海市学校体育课程设置进行比较分析发现，美国学校体育课程设置内容广泛，重视个体全面发展、注重人文关怀、注重各级学校体育课程衔接；提出上海市学校体育课程设置要提高衔接性、体育课程设置应该重视人文关怀，关注残疾人体育、充分利用体制优势，促进学生养成良好体育锻炼习惯、加强学校、家庭和社区的联动，构建完整体育活动圈等建议。以期为学校体育课程改革提供些许有益的参考，从而进一步促进学生身心健康。     

基于XML的科研管理信息系统研究

该文在分析农业科研单位科研管理现状的基础上,提出了一种基于XML技术的科研管理信息系统,并进一步介绍了系统采用的B/C/S结构模型,阐述了系统的功能模块和系统实现的部分关键技术。得出该系统能降低对客户端的要求,减少系统的通讯量,使得数据的呈现、交互更加容易,从而提高了科研管理工作的效率。     

Vineyard soil bacterial diversity and composition revealed by 16S rRNA genes: Differentiation by geographic features

Here, we examine soil-borne microbial biogeography as a function of the features that define an American Viticultural Area (AVA), a geographically delimited American wine grape-growing region, defined for its distinguishing features of climate, geology, soils, physical features (topography and water), and elevation. In doing so, we lay a foundation upon which to link the terroir of wine back to the soil-borne microbial communities. The objective of this study is to elucidate the hierarchy of drivers of soil bacterial community structure in wine grape vineyards in Napa Valley, California. We measured differences in the soil bacterial and archaeal community composition and diversity by sequencing the fourth variable region of the small subunit ribosomal RNA gene (16S V4 rDNA). Soil bacterial communities were structured with respect to soil properties and AVA, demonstrating the complexity of soil microbial biogeography at the landscape scale and within the single land-use type. Location and edaphic variables that distinguish AVAs were the strongest explanatory factors for soil microbial community structure. Notably, the relationship with TC and TN of the <53 μm and 53–250 μm soil fractions offers support for the role of bacterial community structure rather than individual taxa on fine soil organic matter content. We reason that AVA, climate, and topography each affect soil microbial communities through their suite of impacts on soil properties. The identification of distinctive soil microbial communities associated with a given AVA lends support to the idea that soil microbial communities form a key in linking wine terroir back to the biotic components of the soil environment, suggesting that the relationship between soil microbial communities and wine terroir should be examined further.     

Establishment of macro-aggregates and organic matter turnover by microbial communities in long-term incubated artificial soils

The study of interactions between minerals, organic matter (OM) and microorganisms is essential for the understanding of soil functions such as OM turnover. Here, we present an interdisciplinary approach using artificial soils to study the establishment of the microbial community and the formation of macro-aggregates as a function of the mineral composition by using artificial soils. The defined composition of a model system enables to directly relate the development of microbial communities and soil structure to the presence of specific constituents. Five different artificial soil compositions were produced with two types of clay minerals (illite, montmorillonite), metal oxides (ferrihydrite, boehmite) and charcoal incubated with sterile manure and a microbial community derived from a natural soil. We used the artificial soils to analyse the response of these model soil systems to additional sterile manure supply (after 562 days). The artificial soils were subjected to a prolonged incubation period of more than two years (842 days) in order to take temporally dynamic processes into account. In our model systems with varying mineralogy, we expected a changing microbial community composition and an effect on macro-aggregation after OM addition, as the input of fresh substrate will re-activate the artificial soils. The abundance and structure of 16S rRNA gene and internal transcribed spacer (ITS) fragments amplified from total community DNA were studied by quantitative real-time PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE), respectively. The formation of macro-aggregates (>2 mm), the total organic carbon (OC) and nitrogen (N) contents, the OC and N contents in particle size fractions and the CO₂ respiration were determined. The second manure input resulted in higher CO₂ respiration rates, 16S rRNA gene and ITS copy numbers, indicating a stronger response of the microbial community in the matured soil-like system. The type of clay minerals was identified as the most important factor determining the composition of the bacterial communities established. The additional OM and longer incubation time led to a re-formation of macro-aggregates which was significantly higher when montmorillonite was present. Thus, the type of clay mineral was decisive for both microbial community composition as well as macro-aggregation, whereas the addition of other components had a minor effect. Even though different bacterial communities were established depending on the artificial soil composition, the amount and quality of the OM did not show significant differences supporting the concept of functional redundancy.     

Breeding Report of New Mung Bean Variety“Ji Heilv No.12”

The mung bean variety Ji Heilv No.12 was bred by lnstitute of Characteristic Crop Research, Chongqing Academy of Agricultural Sciences and lnstitute of Food and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences. using Jilv 9 and Jilv 7 as female and male parent respectively,with pedigree method. Ji Heilv No.12 is a new variety with features of high and stable yield,broad adaptability and strong resistance in yield trails during 2011-2012; it was approved and released by Chongqing Provincial Committee of Crop Variety ldentification in 2012,suitable for cultivating in most area of Chongqing.     

RF-IC卡门禁系统设计

门禁系统能够给各种需要一定程度管理的场所带来极大的方便.它不仅能制止非法人员的侵入,也可对合法人员的行为时间进行记录,在很大程度上提高了其在安全管理方面的层次,具有较高的现实意义和市场需求.文章主要介绍了RF_IC卡门禁系统,并对系统的总体设计方案作了简要论述.     

鲍曼不动杆菌雏鸡分离株CCGGD201101的分离、鉴定及致病性研究简

试验旨在鉴定吉林省某雏鸡孵育基地病死雏鸡组织中分离出的1株致病性菌CCGGD201101株并测定其致病性。对疑似致病菌进行生理生化试验、16S rDNA测序鉴定，并人工接种昆明鼠，测定其半数致死量，验证细菌毒力。经鉴定该菌为鲍曼不动杆菌（Acinetobacter baumannii）。以鲍曼不动杆菌CCGGD201101分离株为研究对象，并以鲍曼不动杆菌标准株（ATCC 19606）为对照，测得半数致死量，进一步证明鲍曼不动杆菌病死鸡分离株CCGGD201101具有较强致病性。     

基于RFID技术的电动车充电系统

本文设计了基于RFID技术的一种刷卡式的充电服务终端硬件装置,实现刷卡自动计费。本程序以AT89S52单片机为主控制器,用MFRC500射频识别芯片进行识别,并与射频卡进行通信。     

Devastation of sesame (Sesamum indicum L.) crops in different agroclimatic zones of India by genetically diverse subgroups of phytoplasma

The sesame crop is highly susceptible to infection by phytoplasmas, a class of cell wall-less plant pathogenic bacteria (Mollicutes), which is responsible for widespread loss of sesame crops in both North and South India in recent years. Therefore, characterizing the pathogen population is required before the control measures can be devised and implemented. With molecular tools based on nested polymerase chain reaction (PCR) assays, sequencing, restriction profiling, and phylogenetic analysis, phyllody-affected sesame plants collected from nine different states of India were found to be infected by phytoplasmas belonging to two 16Sr groups, namely 16SrI and II. Two subgroups of phytoplasma −16SrI-B and 16SrII-D— were prevalent in symptomatic sesame samples collected from North India, whereas phytoplasma of only the 16SrII group was found in South India. However, the latter samples were diverse, belonging to three different subgroups (16SrII-A, II-C, and II-D). In addition, yearly phyllody-affected sesame samples from Delhi for 4 consecutive years (2007–2010) showed variation in the infecting phytoplasma: the subgroup 16SrII-D was detected in samples collected in 2007, and 16SrI-B was predominantly found in the samples collected in the subsequent years. The study also provides molecular evidence for the association between 16SrI-B phytoplasma and different symptoms in sesame crops such as fasciation, little leaf, and stunting. This is the first study to report the association of the phytoplasma subgroups 16SrII-A and II-D with sesame crops in India. This study provides a baseline for designing specific detection and molecular analysis strategies for quarantine purposes. It also highlights the need for examining the dynamics of seasonal or location-specific variation in vector populations to determine the pattern of infection outbreaks.     

引入6S管理体系提高个体食品生产者职业素质

介绍了6S管理的内涵、实施方案,以及引入6S管理的必要性与可行性。针对当地个体食品生产者生产馒头的现状,引入6S管理体系,取得了良好的效果。