不要把安全帽当凳子坐

编辑同志:近日,笔者下乡时,看到一个很不好的现象:在野外就餐时,部分参与农网改造升级工程的施工队员把安全帽放在地上当凳子坐。问其原因,有施工人员说:"坐在安全帽上既干净、舒服,又不用直接与地面接触,以免患上‘湿疹’等病症。"对此,笔者提醒有关人员:切不可把安全帽当凳子坐。     

红缘短鼻蝗染色体C带核型研究(蝗总科:癞蝗科)

[目的]研究红缘短鼻蝗的染色体C带核型。[方法]利用BSG法进行显带处理,显微摄影后,测量染色体和C带带纹的相对长度,计算每条染色体异染色质的含量。[结果]红缘短鼻蝗的染色体组成为2n♂=19,性别决定机制是XO型,染色体全部为端着丝粒染色体,且仅具有着丝粒C带带纹。此外,异染色质在染色体组中的总含量为16.40%。[结论]该研究为癞蝗的细胞遗传学和细胞分类学提供了染色体及其显带方面的资料。     

鸡蛋中臭鼻克雷伯氏菌的分离与鉴定

从市售鲜鸡蛋内容物中分离到一株革兰氏阴性杆菌,经形态学观察、培养特性观察及生化试验鉴定该菌为臭鼻克雷伯氏菌。药敏试验表明该菌对先锋霉素、链霉素、氯霉素和庆大霉素敏感。动物实验结果表明,该菌对小白鼠有致病力。一般情况下臭鼻克雷伯氏菌属于条件致病菌,从鸡蛋中分离出致病菌株,在公共卫生方面具有重要意义。     

灵验便方四则

1．治湿疹：取苦楝树皮、乌桕叶各200克,水煎洗患处。每日1剂,分2次洗,连洗数日可愈。用本法治痱子亦有良效。     

怎样防治幼兔球虫病

球虫病主要为害1-4月龄幼兔,死亡率很高。 一、症状 幼兔感染球虫病时,腹部膨胀,眼鼻分泌物及唾液分泌增多,精神沉郁,食欲减退。继而交替出现下痢或便秘。夏季多出现下痢,此时．患兔肛门四周被毛不洁,出现贫血、尿频,消瘦,卧地不起,最终衰竭而死亡。有的病例还出现鼻炎或结膜炎症状。球虫病兔愈后仍可带虫,互为感染。     

夏枯草可治小儿湿疹

取夏枯草150～200克,放入2500～3000毫升水中煮沸10～15分钟,去渣,倒入盆中,水温冷却至38℃～41℃。     

妙用麦麸接产子猪

在母猪生产前准备好半盆麦麸。母猪生产时,接产人员用左手握住子猪躯体,使子猪成水平状态,右手立即把子猪口、鼻内的黏液去除,并用干净的布擦净头脸,防止黏液堵塞口鼻,闷死子猪。然后将子猪放入盛麦麸的盆中,往子猪身上撒麸皮,接着用双手从前向后(包括腹下及腿部)搓掉麦麸,这样子猪身上的黏液均被麸皮吸干,子猪身上干干净净。用过的麸皮晒干后还可用来喂母猪,特别是后备母猪,一方面胎内带出的黏液里含有     

冬春季节牛传染性鼻气管炎的诊治

牛的传染性鼻气管炎,是由牛疱疹病毒、鼻病毒引起的一种急性、热性、高度接触性呼吸道传染病,病毒侵入牛体后,导致持续性感染,病牛长期乃至终生带毒,给控制和消灭本病带来极大困难。     

山羊地方性鼻内肿瘤病毒gag蛋白的原核表达、纯化及多克隆抗体的制备

为获得纯化的山羊地方性鼻内肿瘤病毒(enzootic nasal tumor virus of goats,ENTV)gag蛋白和抗gag蛋白的多克隆抗体,根据GenBank已登录的ENTVgag基因序列,设计合成1对特异性引物,应用PCP扩增gag基因并连接于原核表达载体pET-28a(+)中,构建重组质粒pET-gag,经鉴定正确后转化大肠杆菌Rosetta(DE3)株进行诱导表达,并进行SDS-PAGE分析。重组菌经IPTG诱导后成功表达相对分子质量约为69 000的重组蛋白,重组蛋白经镍柱亲和层析纯化、尿素梯度透析复性后免疫小鼠制备多克隆抗体。Western blot试验表明,重组蛋白能与制得的多克隆抗体反应,而与正常小鼠血清和山羊地方性鼻内肿瘤患羊血清不反应。本试验成功获得了纯化的ENTV gag蛋白和小鼠抗gag蛋白的多克隆抗体,为进一步研究gag蛋白在ENTV致病过程中的作用提供了材料。     

Isolation and Identification of Orf Virus from Jiangsu Province and Phylogenetic Analysis of Their B2L Gene

The study was aimed to investigate prevalence of Orf virus (ORFV) in Jiangsu province in recent years and control Orf better. A total of 121 tissue samples were collected in some farms from 2013 to 2015 and subjected to PCR detection, viral isolation and phylogenetic analysis of B2L gene. Four samples were ORFV positive by PCR. The viruses were isolated by passaging in ovine fetal turbinate (OFTu) cells and MDBK cells, and were named as ORFV/Ovis/XZ/Jiangsu/2015/China, ORFV1/Ovis/DT/Jiangsu/2015/China, ORFV2/Ovis/DT/Jiangsu/2015/China and ORFV/Ovis/SL/Jiangsu/2015/China,respectively. The B2L gene was amplified and sequenced for the phylogenetic study. The nucleotide homology of these 4 strains was 98.5% to 100.0%. ORFV/Ovis/XZ/Jiangsu/2015/China, ORFV1/Ovis/DT/Jiangsu/2015/China and ORFV2/Ovis/DT/Jiangsu/2015/China, gathered into a cluster with SC-JY, GX-YB, JS-FX isolates and the nucleic acid homology of these strains was 97.8% to 100%. ORFV/Ovis/SL/Jiangsu/2015/China gathered into a cluster with LiaoNing, HuB and Gansu isolates, the nucleic acid homology was 98.8% to 98.9%. The nucleic acid homologies of 4 strains and ORFV strain China vaccine was 96.8% to 98.1%. The result showed that the ORFVs in Jiangsu province might be from different source. For controlling the spreading of this virus, it was necessary to carry out deep epidemiological survey in Jiangsu province.