cDNA Cloning and Sequence Analysis of Rice Sbel and Sbe3 Genes

Two starch-branching enzyme (SBE) in rice, is known to be a key enzyme in amylopectin biosynthesis. The cDNA of two SBE(starch-branching enzyme) genes SheI and Shed encoding SBE Ⅰ and SBE Ⅲ (two major isoforms in rice) were cloned by an improved RT-PCR technique, from a template cDNA libray, derived from the total mRNAs extracted from the immature seeds of a japonica rice Wuyunjing 7. DNA sequence analysis showed that the size of the cloned SheI and Shed cDNAs were 2490 and 2481 bp long, respectively, including their entire coding sequences. Comparison analysis indicated that the nucleotide sequence of She3 was the same as that of shed (Genbank Accession No. D16201) as reported previously. There were only four base-pairs difference,which resulted in changes of two deduced amino acids between the cloned She1 cDNA and the reported she1 (Genbank Accession No. D11082). The cloned SheI and Shed cDNAs make it possible to improve rice starch quality through genetic engineering.     

木聚糖酶产生菌的筛选及酶学性质研究

从实验室保藏的13株菌株中筛选到l株高产木聚糖酶菌株-黑曲霉2107,酶谱检测发现,该茵株产2种木聚糖酶X-1和X-2.X-1和X-2的最适反应温度都为50℃,并在30～50℃都较稳定.X-1的半失活温度50～55℃,X-2的半失活温度为55～60℃.X-1的最适pH约为5,pH 4～10较稳定;X-2的最适pH约为3和4之间,pH 2～5之间较稳定.各种金属离子对2种酶都有不同程度的抑制作用.     

漆酚热预聚漆研究（Ⅰ）──漆酚热预聚漆酶催化成膜

为了充分让漆酶在最后催化成膜中发挥作用，将生漆分离出漆酚和粗漆酶后，先将漆酚进行热预聚，再用粗漆酶配制成漆酚热预聚漆。试验结果表明：此法配制出的漆液，不仅能在漆酶催化下成膜，而且具有粗漆酶用量少、干燥性能好的特点。     

棉花纤维发育后期Mg2+,K+-ATP酶的活性分析

1989～1990年选用陆地棉(G.hirstum L.)北农1号品种和海岛棉(G.barbadenseL.)海7124品种为材料,对棉纤维发育后期的纤维素形成过程进行了研究。结果表明:①随着棉纤维的发育 Mg~(2+),K~+-ATP 酶活性逐渐下降。②Mg~(2+),K~+-ATP 酶活性的最适 pH 值为6.5。Mg~(2+),K~+ATP 酶活性随[Mg~(2+)],[K~+]和[NADH]/[NAD~+]的增大而变大。③Mg~(2+),K~+ATP 酶活性变化与还原性糖含量变化呈显著正相关(r=0.811~*),与纤维素含量变化呈极显著负相关(r=-0.900~(**))。④Mg~(2+),K~+-ATP 酶参与了纤维素的形成过程。     

新疆驴血液蛋白质(酶)多态性研究

试验采用聚丙烯酰胺和浓度梯度电泳，对新疆驴的白蛋白（Alb)，前白蛋白（pre)、后白蛋白（poA)、运铁蛋白（Tf)以及酯酶（Es)、碱性磷酸酶（AKP）6种蛋白质（酶）位点进行多态性研究。结果表明，运铁蛋白、白蛋白位点表现多态型；前白蛋白、后白蛋白、酯酶均不表现多态型；有碱性磷酸酶的个体占61．11％，无碱性磷酸酶的个体占38．89％。     

应用间接免疫酶组化法诊断鹌鹑新城疫

新城疫(ND)是极易传染的毁灭性传染病,最常侵害鸡和其他禽类,有关鸡新城疫的诊断方法很多,但鹌鹑新城疫的发病特点与鸡新城疫有些差异,有关应用间接免疫组化法诊断鹌鹑新城疫未见报道。我们采用间接免疫组化法检测NDV抗原在人工感染、自然感染病例的患鹑体内的分布特点,以期为诊断鹌鹑新城疫提供依据。 1 材料与方法 1.1 NDV种毒 A株NDV,由我院微生物教研室从病死鹌鹑分离并确证为NDV;F_(48)E_8株NDV由中监所引进。以上两毒株用前经鸡胚传代,分离病毒于