ELISA在牛病毒性腹泻病检测中的应用

牛病毒性腹泻病呈世界性分布,在许多养牛业发达国家尤其严重,造成该病广泛流行的主要传染源是牛群中存在持续性感染牛,因此建立起特异、敏感、简便且快速的诊断方法来检疫、淘汰持续性感染牛是防制该病的关键之一。ELISA因其特异性强,敏感性高,重复性好,方法快速、简便,设备简单等优点,而得到日益广泛的应用,愈来愈受到人们的重视。目前,应用ELISA检测牛病毒性腹泻病毒已有不少研究,主要包括抗原捕获ELISA、间接ELISA、ABS-ELISA、双抗体夹心ELISA、单抗夹心ELISA、竞争ELISA以及M-ELISA等。文章就这几种检测方法的研究进展进行了概述,为进一步控制牛病毒性腹泻病毒提供参考。     

牛网胃瘘管的手术治疗

牛误食尖硬异物造成网胃瘘管者,临床少见。多年来笔者诊疗2例,均用切除剑状软骨,内翻瘘管的方法治愈。现报告如下。     

一例牛瓣胃阻塞的治疗与体会

牛瓣胃阻塞是因前胃弛缓，瓣胃收缩力减弱，内容物在瓣胃内异常干涸，使瓣胃扩张、坚硬、疼痛，导致严重消化不良为特征的疾病。本病奶牛、舍饲牛易发生。     

牛中暑的科学防治

牛中暑是因天气高温、高湿或烈日曝晒而引起的，它是一种全身代谢扰乱，血液循环和中枢神经系统高度障碍的急性病。中暑的病情发生、发展急剧．往往迅速死亡，所以正确的预防、抢救和治疗至关重要。但我们在临床实践中．常见由于误识或采取措施不当．治疗错误等．而致发病率或致死率增高的情况．现就其防治中的若干问题做一简述。     

Study on Proliferation and Apoptosis of EBL Cells Induced by P48 Protein of Mycoplasma bovis

The aim of this study was to investigate the effect of P48 protein on proliferation and apoptosis of embryonic bovine lung (EBL) cells.In this study,samples which co-incubated with P48 protein and EBL cells in different concentrations at different time points were collected,the proliferation rate of the cells was detected by MTT method,and the changes in the nuclear morphology of EBL cells were observed by DAPI staining method.Meanwhile,flow cytometry was used to detect the apoptosis rate of EBL cells induced by P48 protein.Real-time quantitative PCR was used to detect the changes in mRNA level of apoptotic marker genes,and Western blotting tested the Bax and Beclin-1 protein expressions.The results showed that under the condition of 72 h and 10 μg/mL of protein concentration treatment,P48 extremely significantly inhibited EBL cells proliferation (P<0.01),while 0.1 and 0.5 μg/mL protein concentration had no inhibitory effect (P>0.05).The nuclear morphology showed no significant change after protein induction for 12 h,but wrinkled and condensed at 24 h.The nucleus was fragmented,and a sprouted apoptotic body was appeared at 48 and 72 h.Apoptosis related genes expression showed no obvious increase at 2 and 12 h at mRNA level,but gradually increased at 24,48 and 72 h,and it showed a time-dependent manner.Accordingly,the expression of apoptosis marker proteins Bax and Beclin-1 significantly increased.Flow cytometry analysis showed that the apoptosis rate of EBL cells induced by P48 protein was 48.44%.In conclusion,P48 recombinant protein of Mycoplasmas bovis inhibited the proliferation of EBL cells and promoted their apoptosis,which provided reference for revealing the pathogenic mechanism of Mycoplasmas bovis.     

谈杂交牛犊的饲养管理

近年来,肉牛养殖成了农村经济发展的一项支柱产业。牛冻配改良技术的普及应用,加快了养牛业从传统的役用向肉用方向发展,肉牛生产初具规模,但农户容易忽视杂交牛犊的饲养管理,使杂交牛犊的杂交优势得不到充分体现,甚至死亡,造成一定的损失。因此,重视和加强牛犊饲养管理,增     

日本牛的遗传资源

本文扼要回顾了日本牛种资源的发展历史;介绍了日本牛的遗传资源状况和各主要牛种的特征特性;从日本牛的遗传可变性和遗传距离等方面分析了日本牛品种之间的遗传关系;并展望了日本黑毛和牛、日本褐牛、日本短角牛、日本无角牛、见岛牛、口子岛牛等日本牛种资源的发展前景。     

犊牦牛血浆和心肌中心钠素含量的测定

心房利钠多肽(Atrial natriuretic peptide,ANP),又称心钠素,是哺乳动物心房肌细胞分泌的一种肽类激素,具有强大的利尿、利钠、扩张血管、支气管、降低血压和心输出量的作用[1,2].     

浅析荷斯坦牛最高日产奶量与305 d校正产奶量的相关关系

荷斯坦牛最高日产奶量与305d校正产奶量间相关系数r=0．8164（P〈0．01）,最高日产奶量（x）与305d校正产奶量（y）回归方程为y=182．04x＋617．97。用最高日产奶量估计公牛女儿一胎305d校正产奶量,进行后裔测定,可缩短选种周期200d左右,为早期选种提供了依据。     

鸡的天然增色饲料添加剂

1．万寿菊：采集万寿菊花瓣风干后研成细末，在鸡饲料中添加0.3％，可使蛋鸡产出深橙色蛋黄蛋，肉鸡则皮肤呈金黄色。