Inhibition of HSP70 Gene Expression by Modified Antisense and Its Effects on Embryonic Sensitivity to Heat Shock

Experiments were performed to evaluate the efficiency of inhibition of HSP70 gene expression by antisense oligonucleotides complementary to the mRNA of HSPTO and to test the effects of inhibition of HSP70 gene expression on subsequent embryonic sensitivity to heat shock. The results showed that transfection of pre-implantation embryos at 4-cell stage with 5 μM antisense oligo had no effect on in vitro blastocyst development. However, transfection with 10 to 40 μM antisense oligo had reduced in vitro blastocyst development to 15, 10% and 0; For the embryos which exposed to 40 μM As arrested at the 16-cell stage, there was no blastocyst formation within the heat shock groups. In contrast, transfection had no effect on embryonic sensitivity to heat shock, above 25% of embryos developed to blastocyst stage in control groups.     

赤羽病的诊断与防治

文章介绍了赤羽病的危害、流行病学、临床症状及诊断。该病多发生于热带和温带地区，可引起多种动物感染，怀孕的牛、绵羊和山羊对本病最易感。根据流行特点、临床表现和病理变化可做出初步诊断。但确诊必须进行实验室检查(包括病原学鉴定和血清学试验)，其中，以中和试验结果较为可靠。     

我国成功“克隆”天然牛黄

我国消费者今后不必再为买不到传统天然牛黄而发愁了。由武汉生物化学制药厂主持的国家火炬计划项目“体外培育牛黄原料药项目”取得成功，“体外培育牛黄”不久前已经国家食品药品监督管理局批准作为国家一类新药正式生产。有关中药专家指出，体外培育牛黄质优价廉，与天然牛黄具有相同的疗效，是天然牛黄理想的替代品。     

秋冬喂牛有“新巧”

秸秆巧处理 饲草单一营养缺乏．易导致牛掉膘消瘦，如果把秸秆进行氨化处理，牛就爱吃。办法是：把麦草、稻草等细铡成2～3厘米的短节，每100千克碎麦草或稻草加4千克尿素，先用40千克水把尿素溶解后，搅拌在碎麦草、碎稻草内，搅匀后，装人大缸或水泥地，压实、封严，不透空气，进行氨化，50天后可开缸饲喂。氩化过的饲草，绵软．芳香，易消化。     

中国西门塔尔牛新品种选育获奖

中国农科院畜牧所许尚忠研究员主持的“中国西门塔尔牛新品种选育”前不久荣获2003年度国家科技进步二等奖。     

黑龙江西部牛羊东毕吸虫病流行情况调查与防治

黑龙江省西部地区主要包括齐齐哈尔市、大庆市及绥化市的部分县(市)，这里有丰富的草原资源，是黑龙江省重要的牧业生产基地。西部地区自然水域辽阔，江河湖泊众多，为吸虫的中间宿主——螺类的孳生提供了有利的生态环境，这就为吸虫病流行和暴发提供了条件。东毕吸虫病(Orientbilharziasis)是黑龙     

平胃散加减治疗鸡软嗉症

平胃散出自《和剂局方》，兽医临床上通常用于燥湿健脾、宽中理气，治疗牛、羊胃寒慢草等症。笔者将原方加减煎水，让鸡自饮，治疗鸡软嗉症。临床试治蛋鸡患软嗉症数万例，均受到满意效果，现报道如下：     

Effects of Three Drugs on Membrane Metabolism Against Cysticerci cellulosae in vitro

To investigate the effects and mechanisms of the benzimidazole carbamate and benmidine drugs on Cysticerci cellulosae and choose effective drugs on Cysticerci cellulosae, the membrane metabolism of Cysticerci cellulosae in vitro was tested after three kinds of drugs which were used respectively. The indexes included the contents of lipids, the contents of SA and the changes of the membrane fluidity. The results showed that oxfendazole could inhibit the membrane metabolism of immature and mature Cysticerci cellulosae in vitro, and albendazole only inhibited the mature one, while thibendimidine neither acted on the immature nor mature one.     

山东省肉牛羊养殖基地一良育牧业

山东良育牧业是集养殖、育种、科研、推广于一体的大型牧业集团。现已成为山东省养殖规模最大、设施齐全、技术力量雄厚的繁育推广单位。常年向社会供应西门塔尔牛、利木赞牛、夏洛莱牛、鲁西黄牛、改良肉牛、育肥小公牛、肉牛犊。成年牛体重900千克左右、育肥期日增重2．25～2．8千克。     

杂交牛育肥技术

正确选购架子牛 选择国外肉用良种公牛作父本杂交的改良牛。 挑选四肢高，体形长，皮肤松弛有弹性，肌肉较薄，骨架清晰，宽而不丰满，毛密柔软和体况健康的牛。因这种牛生长潜力大，肥育效果好。