临床上犬耳血肿的诊疗

正耳血肿是耳部较大血管破裂,血液流至耳软骨于皮肤之间形成的血肿。多发生于耳廓内面。各种动物均可发生,但多见于猪、犬、猫。耳廓血肿为血液在耳廓的蓄积,病因多为外耳炎引起自身搔抓和摇头所致。1病例简介在9月份实习期间,一斗牛犬来我兽医院就治,据该主人说,此犬名叫点点,雌性,2岁龄,耳廓部肿大,且该犬精神状态不好,不爱吃东西。1.1临床检查经临床检查,该犬耳部增厚、肿胀,触之有捻发音且痛感     

Study on Clinical Efficacy of Oligosaccharide Sulfate and Dextran Sulfate Against Bovine Mastitis

In order to detect the clinical efficacy of oligosaccharide sulfate(DEAE) and dextran sulfate (DS-4000) against bovine mastitis,56 cows with bovine mastitis were divided into 5 groups (DEAE group,DS-4000 group,DEAE＋penicillin group,DS-4000＋penicillin group and penicillin group),and their breasts were perfusion with different drugs twice one day for 3 days.The effective rates of drugs were observed by clinic symptoms.Milk samples were collected before and after treatment of drugs,and the bacterial negative conversion rate of each group were detected by microbial examination.The results showed that total effective rates of 5 groups were 10.00%,20.00%,58.33%,75.00% and 50.00%,respectively,and the bacterial negative conversion rates were 0,12.50%,50.00%,54.55% and 44.44%,respectively.In conclusion,the curative efficiencies of the polysaccharides against bovine mastitis were weak,but it could be improved by combination with penicillin.     

Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.     

浅谈家禽气囊炎发病原因及综合防治

家禽气囊炎是家禽中的一种常见疾病,其危害性较大,治疗困难。文章分析了导致家禽气囊炎发生且难以治疗的原因,同时针对性地提出了综合防治措施。     

奶牛乳腺防御机理及乳房炎防治研究

从非特异性屏障、细胞群防御、体液抗体、乳汁抗菌物质及细胞因子方面,对奶牛乳腺的免疫防御功能进行了综述,并在此基础上论述了奶牛乳房炎的预防和治疗技术。认为乳房炎的复杂性和特异性限制了人们对奶牛乳腺免疫防御机理的全面认识和掌握,进而影响乳房炎的防治研究进程。因此,深入探索奶牛乳房炎的防御机理及防治技术,降低乳房炎的发病率和经济损失具有重要意义。     

奶牛乳房炎的综合防治

奶牛乳房炎一直以来是奶牛场常见病、高发病,也是造成奶牛养殖业损失最严重的疾病。病因复杂,如果不及时治疗就会导致群发,致使产乳量降低,造成较大经济损失。文章从奶牛乳房炎的类型、病因、防治措施等方面对奶牛乳房炎进行了介绍,以期为对奶牛养殖者提供参考。     

奶牛乳房炎的防控措施

奶牛乳房炎是奶牛乳腺因外伤、化学刺激、感染或发炎的一种病理反应,是奶牛的一种多发性疾病,也是奶牛产奶生产中危害最大的疾病之一。乳房炎不仅会造成奶产量、奶品质的下降、治疗费用增加和废弃奶损失,甚至还会导致奶牛快速淘汰,给牛场造成极大的经济损失。文章提出奶牛乳房炎的防控措施应采取预防为主,以防治结合,加强饲养监管和保持舍卫生。     

内质网应激偶联炎性反应研究进展

内质网是真核细胞蛋白合成和折叠的主要细胞器,当内质网内蛋白合成或折叠负担增加,引起未折叠或错误折叠蛋白增多时,可激活内质网几条特定信号通路,启动未折叠蛋白反应,这对维持细胞稳态有重要意义。越来越多研究表明,多种炎性反应疾病与内质网应激有密切联系。一方面,内质网应激引起的未折叠蛋白反应可以诱发或者抑制炎症,另一方面,炎性反应也能影响蛋白折叠,从而促进或缓解内质网应激。2型糖尿病、肿瘤和动脉粥样硬化等多种重大疾病的病理机制都涉及内质网应激与炎性反应的相互作用,对该问题的深入研究不仅能加深人们对这些疾病发病机制的理解,也有助于相关药物的研发。     

甘肃省某奶牛场肠杆菌性乳房炎主要病原菌的分离鉴定与耐药性分析

为查明甘肃省某奶牛场发生临床型奶牛乳房炎的主要病原菌并选择治疗用敏感药物,随机无菌采集了6份临床型病牛乳样和1份大罐乳样,采用划线分离、菌落形态观察、革兰染色镜检、生化鉴定及16S rDNA序列分析等方法进行细菌分离鉴定,同时采用K-B法测定主要分离菌株对抗菌药物的敏感性。结果表明,从临床型乳房炎乳样中分离鉴定出大肠埃希菌、芽胞杆菌、凝固酶阴性葡萄球菌(腐生葡萄球菌)3种细菌,其中大肠埃希菌的分离率最高,为66.67%;从大罐奶样中也分离出大肠埃希菌。药敏试验结果表明,大肠埃希菌对氨苄青霉素、阿莫西林、头孢噻吩、杆菌肽、红霉素均存在不同程度的耐药,耐药率为29%~100%;对诺氟沙星、恩诺沙星、氟苯尼考、头孢哌酮等药物敏感。说明此次牛场临床型乳房炎的主要病原菌是大肠埃希菌、葡萄球菌和芽胞杆菌,而且分离的大肠埃希菌具有多重耐药性。根据检测分析结果,给奶牛场提出针对性的防控措施,临床型乳房炎的发病率下降,病情得以控制。     

高剂量酒糟摄入对山羊肾脏组织氧化指标、炎性因子、转录谱的影响

旨在探索高剂量酒糟摄入对山羊肾脏组织氧化指标、炎性因子、转录谱的影响,为揭示高剂量酒糟饲喂对山羊肾脏损伤的机制提供理论基础。选取6只30日龄荣昌本地山羊随机分为2组,每组3只,对照组按照《肉羊饲养标准》(NY/T816-2004)推荐的山羊饲养标准饲喂;试验组用酒糟代替日粮中75%蛋白质和能量成分,试验期为90 d。90 d后采集山羊肾脏组织,利用生化法测定山羊肾脏组织T-SOD、CAT、GSH-Px、XOD、T-AOC含量,用ELISA法测定山羊肾脏组织IL-6、IL-1β、TNF-α含量,用RNA-Seq技术进行转录组测序分析。结果显示,试验组XOD水平显著低于对照组(P<0.05),T-SOD和T-AOC水平极显著低于对照组(P<0.01)。试验组IL-1β水平极显著高于对照组(P<0.01),IL-6、TNF-α水平显著高于对照组(P<0.05)。与对照组比较,试验组共发现546个差异表达基因,其中有385个基因表达上调,161个基因表达下调,qRT-PCR显示挑选的基因与转录组测序结果的表达趋势具有一致性。GO富集分析显示试验组差异表达基因在生物过程中...