抗热应激中草药添加剂在蛋鸡和肉鸡生产中的应用研究

随着集约化高密度养鸡方式的迅速发展,在我国南方高温、高湿地区,养鸡业因夏季热应激对鸡生产的影响已成为急待解决的问题。过高的温度会导致鸡体的热应激综合症,表现生理活动改变、血液和尿液生理生化指标改变、采食量下降、生产性能降低、抵抗力衰弱,严重时热休克死亡,造成较大的经济损失。     

血红蛋白脂质体治疗犬急性失血性贫血效果的观察

为观察人工制备血红蛋白脂质体(LHb)对急性贫血患犬的治疗效果,使用脂质体包埋犬血红蛋白,建立犬急性失血性贫血病例模型。12只犬随机分成4组,每组3只:空白组(Sham)、输入血红蛋白脂质体组(LHb)、输入自体全血组(SAB)、输入生理盐水组(Saline),分别观察各组供试犬处理前后的临床表现,同时测定血常规、血生化及尿常规指标。结果表明,LHb与SAB试验犬相对于Sham及Saline组试验犬,临床贫血症状均获明显改善;第1天时,LHb试验犬血红蛋白浓度(167.67±2.96)g/L明显高于失血前(135.33±3.18)g/L,第3天开始与SAB组差异不显著(P0.05)。LHb组白细胞(WBC)与嗜中性(Gran)及单核白细胞(Mono)数前2 d的变化与SAB组差异显著(P0.05)。输入LHb后,血清总胆红素浓度升高并在1~2 d内超出正常参考范围,1~4 d内与SAB组比较差异显著(P0.05);尿液指标显著升高,持续4~5 d。本研究表明,LHb作为血液替代品用于贫血治疗具有明显的效果。     

BI-2000图像分析系统在家兔失血性休克实验中的应用

家兔失血性休克实验是《医学机能学》实验中的一个重要组成部分,在日常教学过程中,传统经典实验只观察血压的变化,针对这一问题,我院引进BI-2000图像分析系统,并在2014级临床医学教改班家兔失血性休克实验中应用,使学生对于休克发生、进展的机制有了更深刻的理解,对休克各期微循环血液灌流的变化特点有了更直观的认识。     

应用休克指数预测肝/脾破裂患者失血量对指导急救与护理的临床意义

目的探讨应用休克指数预测肝/脾破裂患者失血量对指导急救与护理的临床意义。方法选择急诊住院且初步诊断为肝/脾破裂患者56例,根据血压数值计算休克指数(SI),再根据SI预测出血量并判断休克程度,并以此结果进行针对性急救、护理及手术。结果根据SI计算方法,本组56例患者中SI1者1例(1.8%),SI=1者42例(75.0%),SI1者13例(23.2%)。根据手术前SI预测出血量与手术中收集实际出血量比较,基本符合者54例(96.4%)。56例肝/脾破裂患者根据SI进行了急救与护理后,均及时采取了手术治疗,术后未发生并发症,且均痊愈出院。结论采用SI评估肝/脾破裂患者出血量及休克程度,方法简便易行,准确度较高,以此为依据对肝/脾破裂患者进行针对性的急救、护理及手术有积极的临床意义。     

犬骨盆骨折的内固定治疗

由于犬车祸的发生率的增高,骨盆骨折成为常见的损伤之一,仅次于四肢和脊柱骨折,并发症较多,有较高的伤残率和病死率。低能损伤引起的骨盆骨折,多为稳定骨折,临床处理比较容易。而高能损伤的骨盆骨折常较严重,均有不同程度的失血性休克及合并伤。骨盆的畸形愈合及前后环的不稳定会遗留髂痛跛行、下肢短     

疣鼻天鹅失血性休克的治疗体会

在圈养野生动物疾病诊治过程中,由失血引发的休克往往因处理不及时而导致动物死亡.本文以北京动物园圈养疣鼻天鹅缺血性休克为例,介绍采用临床诊断与化验室诊断相结合的方法,在休克初期预判动物发生心衰,并快速制定治疗方案,经过12 d的连续治疗,最终使患病天鹅得以痊愈的治疗体会.     

热应激诱导畜禽氧化应激、热休克反应与免疫和炎症反应的机制及营养调控措施

热应激会引起畜禽机体代谢增强,从而引起活性氧(ROS)的过量产生而发生氧化应激;氧化应激可启动热休克反应,上调热休克蛋白(HSPs)的转录与表达;ROS和HSPs两者都可以激活核转录因子-κB(NF-κB)进而启动免疫和炎症反应。本文综述热应激引起畜禽氧化应激反应、热休克反应与免疫炎症反应的机制及三者之间的相互关系,并在此基础上总结一些营养调控缓解热应激的措施,以期认识这3种反应在畜禽热应激营养调控过程中的意义。     

Effect of Se and VE Supplement on Semen Quality,Antioxidant Enzyme Activities and Heat Shock Protein Expression of Goat in High Temperature Season

This experiment was conducted to study the effect of selenium and vitamin E supplement on semen quality, antioxidant enzyme activities and heat shock protein expression of goat in Hainan high temperature season.16 adult Hainan Black goat with good health and approximate weight were randomly divided into 4 groups, fed with basal diet(control group), basal diet+0.5 mg/kg Se(Se group), basal diet+100 mg/kg VE(VE group), and basal diet+0.5 mg/kg Se+100 mg/kg VE(Se+VE group), respectively.The experimental period was 93 d.Semen samples were collected in the last week of the experiment on two consecutive days.The semen quality, antioxidant enzyme activities and heat shock protein expression were analyzed.The results showed that compared with control group, the ejaculate volume was not significantly affected by Se or VE supplement(P>0.05).Sperm density and sperm motility were increased significantly by Se and VE supplement(P<0.05), and the abnormal rate was decreased extremely significantly(P<0.01).The goats fed with Se and VE also had higher activities of GSH-Px(P<0.01), SOD(P<0.05), CAT(P<0.05) and T-AOC(P<0.01), and lower MDA concentration in seminal plasma(P<0.05).The relative expression levels of HSP70 and HSP90 mRNA in supplement groups were decreased extremely significantly(P<0.01).However, there were some certain differences between the Se and VE supplement groups on semen quality and heat shock protein expression.In conclusion, the supplementation of Se and VE could help to improve goat semen quality by increasing the sperm density, sperm motility, the antioxidant enzyme activities, and decreasing the abnormal rate in hot season of Hainan.Finally, Se and VE supplement had good effects on relieving the environment heat stress.     

耕牛代谢性酸中毒的治疗

耕牛代谢性酸中毒是由于过食精料而引起瘤胃内环境改变、乳酸增多、脱水、血液浓稠等一系列变化的疾病,患畜以呈现消化紊乱、瘫痪和休克为特征。本病乳牛多发,但在农忙季节本站也曾收治过4例耕牛患畜,现将诊治情况介绍如下。