猪源嗜酸乳杆菌La-0231分离鉴定及其特性分析

为获得一株可开发兽药或饲料添加剂的嗜酸乳杆菌菌株,试验采用仔猪新鲜粪便,从菌落形态、菌体显微形态、革兰氏染色着手,分离、纯化菌种.通过生理生化特性和16SrDNA基因序列分析鉴定菌种.采用K-B法,测定菌株对抗生素的敏感性;并对该菌株的毒力进行了测试.结果显示分离鉴定确认的嗜酸乳杆菌,命名为La-0231菌株.其对30...     

影响奶牛体细胞数（SCC）的因素

体细胞数（Somatic Cell Count，SCC）通常是直接反映奶牛乳房健康状况的指标，它的主要成分是白细胞。健康奶牛乳腺中不同类型的体细胞为：巨噬细胞60％，淋巴细胞25％，嗜中性或多型核嗜中性白细胞15％。而感染乳腺的乳汁中约99％的细胞是白细胞，其余为乳房组织分泌细胞。     

灌丛草地放牧山羊的牧草适口性与嗜食性及山羊采食率研究

通过对放牧山羊的牧草适口性，嗜食性和采食率及其相互关系的分析，表明山羊放牧对灌丛草地的植物适口性、嗜食性比率和采食率均以灌木嫩枝叶，中禾草和苔草属植物较优。季节性规律为：春季山羊喜哺食灌木嫩枝叶，灌木嗜食性比率较高，采食率也较高，夏季山羊喜草本植物，草本植物的嗜食性比率高于灌木，秋季山羊较喜啃食灌木枝叶，嗜食性比率灌木高于草本植物，采食率低于春季，灌木采食率高于草本植物。     

绵羊刚果嗜皮菌病研究进展

<正>绵羊刚果嗜皮菌病是由刚果嗜皮菌(Dermatophilus congolensis)引起各种动物及人的一种皮肤传染病,曾称绵羊真菌性皮炎、疙瘩羊毛病、羊毒样腐蹄病、皮肤雨伤病,以头部、腹部和四肢皮肤形成紧密粘着的毛结和红棕色痂块性皮疹为特征。1病原学嗜皮菌属于嗜皮菌科(Dermatophilaceae)嗜皮菌属(Dermatophilus)的成员,菌体结构因培养基类型及培养时间不同而不同,有菌丝期和孢子期两个     

鸡传染性支气管炎病毒分子变异机制的研究

鸡传染性支气管炎(IB)目前已成为养鸡业中危害最大的病毒性传染病之一。病毒基因组RNA的点突变、缺失、插入和不同毒株基因组间的同源重组所导致的病毒血清型、基因型和组织嗜性的改变是造成现用疫苗免疫失败的主要原因。病毒的变异已成为当前对IBV病原学及本病免疫防制研究中的热点，作者重点就IBV变异机制及其变异株的来源等方面作一综述。     

Identification and Pathogenicity Analysis of Aeromonas caviae M12 from Fox

We tried to identify the bacteria and explore the mechanism of the bacteria's pathogenicity via housekeeping gene gyrB and in vitro organ culture (IVOC) of ileum and intramuscular injection. Microscope, electron microscope and scanning electron microscope were also used to observe and the structure, pathogen of M12 and changes of infected tissues. The results showed that M12 was short gram-negative bacteria, and both ends of it were obtuse and the size without flagellum was (0.6 to 1.6) μm×(0.6 to 0.7)μm while length of flagellum was about 2 to 3 times of the length of bacteria.It had 100.0% similarity with Aeromonas caviae from GenBank. IVOC test observed M12' adhesion in intestinal epithelial cell, resulting in formulation of biofilm structure and damage in intestinal epithelial cell. Histopathologic examination showed that M12 could cause damage in intestine, liver, lung, kidney, muscle tissue of rabbit.The assay would offer references for researching the pathogenic mechanism of Aeromonas caviae.     

Isolation and Identification of Aeromonas hydrophila from the Case of Channel Catfish Bowel Disease

To ensure the cause of suspected bacteria disease for channel catfish, the bacteria from clinical cases were isolated and cultured, then the biochemical and molecular biology assay, and animal regression test were used to identify the species of the isolated bacteria, bacteria medicine sensitive test was used to detect the bacterial drug resistance. The results showed that the bacteria were gram negative bacilli. The colony morphology and biochemical characteristics of the isolated bacteria were same as Aeromonas hydrophila. The sequencing test of the 16S rDNA also confirmed the isolated bacteria were Aeromonas hydrophila. The isolated bacteria could infect the healthy channel catfish and cause the same symptoms as the clinical case. The isolated bacteria were sensitive to floroquinolones and resistant to penicillin and cephalosporin drugs. The results showed that the pathogen of case catfish bowel disease was Aeromonas hydrophila, and the floroquinolones could use to prevent and treat the disease.     

牛异嗜癖发生的原因及防治方法

牛喜欢采食正常状态下所不食的物质（非饲料成分）,就称为异嗜癖,也称采食恶癖。牛若经常食毛,舔食啃吃煤渣、墙土、砖块、破布等物,是发生异食癖的表现。1原因分析异嗜癖的发生,主要是牛体内某些营养物质缺乏,机体代谢功能紊乱,味觉异常所导致的比较复杂的综合性疾病。所缺乏的物质可以是矿物质（特别是微量元素）,如钠、钴、铁、硒、钙、铜、硫、锌、锰等,也可能为维生素,特别是B族维生素、维生素E等。     

猪,犬感染犬旋毛虫后其嗜酸性粒细胞的变化情况

猪，犬感染犬旋毛虫后，其体内的嗜酸性粒细胞显著增高，而基佗各类细胞无明显变化，嗜酸性粒细胞于感染后第７ｄ开始升高，１４～３５ｄ之间达到高峰，以后呈逐渐下降趋势，但仍比对照组高，嗜酸性粒细胞的增多可作为早期诊断旋毛虫病的重要临床指标之一。