肉鸡安全无害用药有窍门

<正>1根据肉鸡的生理特点用药肉鸡无汗腺,用解热镇痛药抗热应激效果不理想;肉鸡缺乏充分的胆碱脂酶贮备,对抗胆碱酶药非常敏感,所以驱除肉鸡线虫最好选用左旋咪唑;肉鸡的肾小球结构简单,有效过滤面积小,对肌注后经肾排泄的庆大霉素、链霉素非常敏感;鸡有丰富的气囊,气雾给药效果好。     

家禽用药的原则及常见误区分析

1用药原则1.1要根据家禽的生理特点用药禽类无汗腺,用解热镇痛药抗热应激效果不理想;禽类缺乏充分的胆碱脂酶贮备,对抗胆碱酶药非常敏感,故驱除家禽线虫最好选用左旋咪唑;禽类的肾小球结构简单,有效过滤面积小,对肌注后经肾排泄的庆大霉素、链霉素非常敏感;鸡有丰富的气囊,气雾给药效果好。     

松树增脂剂增脂效果研究

松树增脂剂应用于湿地松的研究表明：施用松树增脂剂后，湿地松平均增脂率达２６．９７％，投入产出比为１：１５．９，在正常情况下，每次施药的增脂效果随着全年施药次数的增加而增加，在同龄林中，林木个体越大，增脂效果越明显。     

云南景谷地区松脂研究的现状与对策

云南省景谷地区具有丰富的思茅松资源，盛产松脂，为此开展了高产脂种源培育等试验研究，对松树进行多种采脂方法的研究，调查各种采脂方法的实用性，并不断开发新的采脂方法。     

土鳖虫冻干粉对高脂血症家兔模型的降脂作用

<正>传统中药土鳖虫性寒味咸,具有逐瘀破积、通络理伤之功效,为鳖蠊科昆虫地鳖(Eupolyphagasinensis Walker)或冀地鳖[Steleophagaplancyi(Boleny)]的雌虫干燥体[1]。土鳖虫的主要成分为氨基酸[2]、生物碱、挥发油和多种脂类成分[3]。临床上,土鳖虫在高脂血症、血栓性疾病和心脑血管缺血性疾病的治疗等方面被广泛应用[4]。随着其在医药方面的广泛应     

Effect of Nutrient Restriction and Compensation on Weight,Serum Indexes and Gene Expression of Chest Fat Adipokines in Mongolian Lamb

To study the effect of nutrient restriction and compensation on weight,serum indexes and gene expression of chest fat adipokine in Mongolian lamb,thirty male lambs were randomly divided into control group (20.26 kg± 3.63 kg) and feed restricted group (21.29 kg±3.00 kg).The experiment lasted for 120 days,including 60 days for feed restriction and 60 days for compensatory feed.At the end of each stage,five lambs from each group were slaughtered and chest fat was collected.Serum concentration of triglycerides,non-esterified fatty acids (NEFA),total cholesterol were measured.Quantitative Real-time PCR were used to determine Adiponectin,Visfatin,PPAR-γ and ACC genes expression.The results showed that:①The lamb body weight of restricted group kept maintain level and that of control group showed a increase tendency.During the compensatory period,the average daily gain (ADG) of restricted group was significantly higher than control group (P< 0.05).②During the restriction period,serum triglycerides concentration of lambs in restricted group was significantly decreased at day 30 and 60 (P< 0.05),and NEFA concentration was significantly increased at day 30 (P< 0.05).After 60 days of nutritional compensation,all serum parameters we tested were not significantly different between restrict group and control group (P >0.05).③During the restriction period,PPAR-γ and ACC gene expression in restricted group were significantly lower than that of control group (P< 0.05),while no significant differences were found during the compensation period (P>0.05).Adiponectin and Visfatin genes expression in chest fat was not significantly changed throughout the experiment (P >0.05).We could know that nutritional restriction reduced fatty acid synthesis and adipocyte differentiation ability,adipose tissue was mobilized energy metabolism,and total cholesterol was recoveried growth on the compensation.However,the ACC and PPAR-γ genes in restricted group were significantly decreased with the body weight increased rapidly during the compensatory stage.Adiponectin and Visfatin were not significantly different between restricted and compensatory stages.     

藏羊脂联素基因多态性及其与产肉性能的相关性分析

利用高分辨率熔解曲线技术对176头2周岁的甘肃藏羊（欧拉型、甘加型、乔科型）脂联素基因SNPs位点进行检测,运用GLM模型将检测到的SNPs位点与部分胴体及肉质性状的相关性进行了分析.结果表明：在脂联素第2外显子发现＋67G＞C突变使编码氨基酸由谷氨酸突变为谷氨酰胺;GG、GC基因型个体的宰前活重、胴体重均显著高于CC型（P＜0.05）.说明脂联素基因该位点可能是影响藏羊胴体及肉质性状的主效QTL或与之紧密连锁,可作为藏羊高档羊肉生产的候选分子标记.     

生鲜乳质量指标检测结果及季节性变化分析

<正>2011年黑龙江省启动建设生鲜乳质量与安全第三方检测体系,实施生鲜乳第三方检测工作。到2012年末,我省已在全国率先建立了功能完备的省级检测中心、黑龙江省生鲜乳第三方检测数据系统,以及71处基层生鲜乳第三方检测实验室,并全部配备了乳成分分析仪、乳房炎检测仪、乳品质量安全检测仪等仪器设备。全省各地共选送培训     

乙烯利和脱落酸对北五味子木脂素及苯丙氨酸解氨酶活性的影响

以北五味子为试材,在转色期分别用400mg/L乙烯利和1 000mg/L脱落酸(ABA)处理果实,研究乙烯利和ABA对北五味子木脂素及苯丙氨酸解氨酶(PAL)活性的影响。结果表明:转色期至成熟处理,对照果实总木脂素和PAL活性均呈增加的变化趋势,成熟期果实总木脂素乙烯利和ABA处理比对照分别提高11.08%和15.11%,处理间无显著差异,与对照差异显著;CK成熟期果实PAL活性为20.21U·h-1·g-1,乙烯利和脱落酸对提高北五味子果实总木脂素含量和增加PAL活性具有促进作用,乙烯利和ABA处理的果实PAL活性分别为22.09、22.36U·h-1·g-1,处理间差异不显著。     

(捺)叶片β-羟脂酰-CoA脱水酶(HCD)基因全长cDNA的分离与表达研究

以(Prunus salicina Lindl.var.cordataJ.Y.Zhangetal)5个不同生长发育时期叶片为试材,根据已构建的5个不同生长发育时期叶片的均一化全长cDNA文库,分离得到了一个羟脂酰-CoA脱水酶(HCD)基因,命名为PsHCD。对PsHCD基因生物信息进行分析,同时利用Real-time PCR检测PsHCD基因在叶片中5个不同生长发育时期的表达量。结果表明:该基因的cDNA开放阅读框编码区为第322个核苷酸到第987个核苷酸,编码221个氨基酸残基,5′UTR长度为321bp,3′UTR长度为156bp。利用TMHMM server软件分析,该基因编码的蛋白质有4个跨膜区域,PSORTⅡPrediction软件分析推测,亚细胞定位于内质网、微体和溶酶体中。实时荧光定量PCR结果表明,PsHCD的表达量随着叶片生长逐渐升高,在展开叶时达到最高峰,在幼叶到成熟叶时处于一种稳定水平,随后降低,老叶表达量最低。