Study on Differentiation of Sheep Umbilical Cord Mesenchymal Stem Cells into Muscle Cells Induced by Mouse MyoD Gene

In order to investigate the differentiation of sheep umbilical cord mesenchymal stem cells (UCMSCs) into muscle cells induced by mouse MyoD gene. This study based on the previous work constructed the eukaryotic expression vector of MyoD-pcDNA3.1 in mice, and the vector was transfected into sheep UCMSCs. The morphological changes of cells were observed by fluorescent microsco, the expression of MyoD, Desmin and MyoG genes were detected by immunofluorescence, the percentage of cells expressing the cell specific factor (MyoD, Desmin and MyoG) was analyzed by flow cytometry and Real-time quantitative PCR to detect the relative expression of mRNA relative to muscle cell specific factor. Compared with the control group (no transfection), the vector was transfected into sheep UCMSCs, it was found that the cells were transformed into a long, slender, muscular cell state, and the cell spiral gradually disappeared at 21th day. It was found that MyoD and Desmin showed positive expression by immunofluorescence assay at 8th day, the expression of MyoG was also found after 16 d of induction, and the expression of MyoD decreased, the amount of Desmin expression was no change;By flow cytometry, the percentages of the expression of MyoD, MyoG and Desmin were 93.5%, 97.4% and 99.5%,respectively;Real-time quantitative PCR results showed that the relative expression of MyoD, MyoG and Desmin were increased and compared with the control group (non transfected cells), the cells were increased by 2.046, 2.389 and 5.489 times, respectively. The results showed that mouce MyoD gene could induce the differentiation of sheep UCMSCs into muscle cells.     

新生犊牛经脐带感染疾病的防治

新生犊牛的扎脐消毒,是接生助产、产犊育幼工作中不可缺少的重要环节。近几年来,随着个体养牛业的发展,新生犊牛出生后的扎脐消毒工作大     

哺乳仔猪的饲养管理

1尽快吃足初乳 初乳中含有丰富的营养物质和免疫抗体,对初生仔猪有特殊的生理作用。仔猪出生后应立即擦干粘液,断脐带消毒后,立刻帮助吃初乳。仔猪能及时吃足初乳,可增强体质和抗病能力,从而提高对环境的适应能力。初乳中含有较多镁盐,具有清泻性,可促进胎便排出。由于初乳的酸度高,可促进消化道的活动。初生仔猪由于某些原因吃不到初乳,很难成     

春季舍饲羊的饲养管理措施

春季羊只瘦弱抵抗力低,母羊还要泌乳喂养羔羊,易引起羊感染各种疾病。1羔羊的饲养管理1.1护理羔羊出生后,脐带以自然断裂为好。如脐带未断,应在距羔羊腹部5~8cm处将其拧断,然后用5%的碘酒涂抹断端进行消毒,忌用剪刀、刀片切断脐带,也不能将脐带结扎。刚出生的羔羊身上有许多黏液,这些黏液要尽量让母羊舔干,也可用干布或草擦干。擦时,首先应将其口、鼻、耳内的黏液擦净。羔羊出生2~4h,要尽快让其吃上初乳,以促进胎     

奶犊牛的人工养护要点

随着奶牛养殖效益的提高,人们开始越来越重视奶犊牛的培育,因为只有搞好奶犊牛的培育,才能切实提高奶牛饲养的经济效益。因此,对刚生下来的小奶牛应及时采取科学的人工养护措施,以确保其健康生长。 1 喂奶要定温、定时和定量 初生牛犊在初生两小时内必须吃到初乳。初生前3周的牛犊,瘤胃、网胃和瓣喂均未发育完全,此阶段不能把牛犊看成反刍家畜,初生后头几周必须以牛奶制品     

提高犊牛成活率3法

一、尽早哺喂初乳母牛分娩后5-7天产生的乳叫初乳。它是新生犊牛不可缺少的营养品。初乳可以抑制和杀灭多种病菌。初乳含有较多的镁盐,可以促进初生犊牛胎粪的排出。哺喂初乳越早越好,即在母牛分     

马胎儿脐带扭转引发流产病例报告

马胎儿脐带扭转引发流产病例报告郭顺元郭建顺李振武（解放军农牧大学动医系·长春·１３００６２）自发性流产为胎儿及胎盘发生反常或直接受到影响而发生的一种流产。我们兽医院门诊发现一罕见的自发性流产病例。原因为马胎儿脐带发生过度扭转（达１３周），并发脐带缠绕...     

母猪常见产科病的诊断与预防

1难产相对于其他家禽家畜来说,母猪的难产的几率相对比较低,主要有生产能力弱、生产通道窄和胎儿有异样三种类型。[1]判断要点:负责管理的员工能够准确的掌握生产时的辅助生产、剪断脐带和对脐带的杀菌消毒的基本技术。如果在生产能力正常、阴部门道松紧正常的状况下,生产不能顺利时就很有可能发生生产困难,较多的情况是腹中的猪仔有异样。要按照标准的消毒程序后方可进行手进生产通道进行进一步的诊断。如果是因为生产能力弱应该进行在垂体后     

初生犊牛常见病的防治

1脐带炎脐带炎是母牛在分娩产仔时由于断脐消毒不严,被细菌感染后而引起脐带发炎和周围组织发炎的病症。患脐带炎的犊牛,其主要症状是精神沉郁,食欲不振,体温升高,呼吸和脉搏加快,触诊犊牛脐带时犊牛有疼痛感,并可在皮下摸到笔杆粗的索状物。脐孔周围出现红肿,挤压时有浓汁流出。如出现坏疽,脐带断端湿润、肿胀,浓汁带有恶臭味时,即为脐带炎初期,可在脐孔周围注射青霉