激发积极情绪或可治癌

<正>【美国石英财经网站7月28日报道】题:在小鼠身上进行的新研究表明,有朝一日可通过激发积极情绪治疗癌症。研究该如何对抗危险的癌症的科研人员正在探寻一条新的治疗之路,可以简短总结为让人感受快乐。目前这种疗法只在小鼠身上进行过试验,但越来越多的研究中的最新一项表明,我们有朝一日也许可以通过激活大脑中的一个主要奖励区域来治疗癌症。这项最新研究来自以色列。位于     

鸡球虫病综合防控

正鸡球虫病是由各种艾美尔球虫寄生于鸡的肠管上皮细胞引起的,以15天—50天的小鸡最易感染,而且患病严重,死亡率很高。球虫卵囊呈圆形或椭圆形,大小只有十多微米,内含一个球形的卵囊核。随鸡粪排出的球虫卵囊,在外界发育成感染性卵囊,鸡随饲料、饮水吃入这种卵囊后,它们就在鸡的肠上皮细胞内,并进行多次反复繁殖很多小虫体,使上皮细胞受到破坏,而引起球虫病发作,最后形成卵囊,破上皮细胞进入     

崂山奶山羊乳腺上皮细胞系的建立及鉴定

实验采用组织块培养法、高密度培养和连续传代法等对崂山奶山羊乳腺上皮细胞进行培养、传代及鉴定。在对纯化的乳腺上皮细胞进行形态观察和生长动力学分析的基础上,进行染色体核型分析、免疫组化、质粒转染等方面的研究。结果表明:利用组织块培养法能够有效地进行崂山奶山羊乳腺上皮细胞的培养,并已传至40代,核型分析其染色体众数为60条,证明得到具有正常形态的崂山奶山羊乳腺细胞;进一步免疫组化显示培养细胞的波形蛋白、细胞上皮膜抗原、角蛋白和β-酪蛋白等上皮细胞标记蛋白呈阳性,证明该细胞为崂山奶山羊乳腺上皮细胞;最后油红染色可见到明显的脂肪滴,外源质粒PEGFP-N1-H-FABP能够稳定地在培养细胞中表达,表明所获得的细胞具有分泌功能。由此可见,利用组织块培养法能够成功建立崂山奶山羊乳腺上皮细胞系,并且所培养的乳腺上皮细胞具备一定的泌乳潜能。     

奶牛乳腺上皮细胞的培养及NOD2定位

为获得纯净的奶牛乳腺上皮细胞并对细胞中的NOD2受体进行定位,本试验采用胶原酶Ⅰ消化法培养奶牛乳腺上皮细胞,通过免疫荧光技术对细胞进行鉴定并对细胞中NOD2进行定位。结果显示:本试验获得了纯净的奶牛乳腺上皮细胞;NOD2受体多表达于奶牛乳腺上皮细胞的细胞核中,且以核仁的表达量最高。     

影响根癌农杆菌转化效率的因素综述

根癌农杆菌介导转化法是植物基因工程的重要方法,但其转化效率受多种因子的影响,包括化学物质、物理方法、外植体遗传特性等,涉及农杆菌与植物互作的各个阶段。将这些影响因子及其功能进行简要综述,以期为优化农杆菌介导植物遗传转化流程提供帮助。     

Isolation,Culture and Identification of Buffalo Mammary Epithelial Cells from Milk

This study was aimed to set up a simple, economic and pure method to culture and identify buffalo mammary epithelial cell in vitro, which were collected from the fresh milk. The milk was collected in the late lactation period of the high yield milk buffalo, then it was mixed with the PBS in the ratio of 2:1 and centrifugated. A few suspernatants and the pellets were resuspended with the PBS when the milk fat were decanted, then recntrifuged. The final pellets and 1 mL of suspernatants were seeded in petri dish without fat, respectively. The isolated cells were mammary epithelial cells which were identified by cell morphology, immunofluorescence, growth curve, cell viability and RT-PCR. The buffalo mammary epithelial cells were successfully isolated from both the pellets and supernatants. The cells and impurities were less in the supernatants than that in the pellets. The cells were cobblestone-like without fibroblasts and most in cell division. In the post-confluent culture, mammary epithelial cells formed dome structures and layer-separated growth. The cells expressed cytokeratin 18 was identified by immunofluorescence which was the marker of mammary cells. The cell growth curve and cell survival rate were measured. The results showed that the buffalo mammary epithelial cells were activity and easy to attach. The mammary epithelial cells were expressed of milk protein and milk fat related genes detected by RT-PCR. The buffalo mammary epithelial cell line were successfully isolated and identified from fresh milk, which estabilished foundation for the study of the mechanism of lactation, the amelioration of the quality of milk and the improvement of milk yield of buffalo.     

干扰ELOVL6基因对奶牛乳腺上皮细胞脂代谢相关基因的表达及甘油三酯合成的影响

为揭示超长链脂肪酸延伸酶6（ELOVL6）对奶牛乳腺上皮细胞脂代谢及甘油三酯含量的影响，试验采用PCR技术扩增得到ELOVL6基因CDS区，利用在线软件对蛋白的疏水性和跨膜区进行预测，合成并筛选靶向ELOVL6基因的有效siRNA，将有效siRNA转染乳腺上皮细胞，qRT-PCR检测干扰ELOVL6基因对奶牛乳腺上皮细胞乳脂代谢相关基因表达的影响，利用试剂盒检测细胞内甘油三酯含量。结果表明：（1）克隆得到全长为795 bp的奶牛ELOVL6基因（GenBank 登录号：MW960011）的CDS区|经序列比对发现，奶牛的ELOVL6基因序列与牛（NM_001102155.1）、山羊（NM_001314257.1）、大羚羊（XM_040237469.1）的同源性分别为100%、96.73%、96.35%|ELOVL6蛋白的理论等电点为5.94，疏水最大值为2.467，最小值为-2.411，具有6个跨膜区域。（2）成功筛选到靶向ELOVL6基因的siRNA,干扰效率达到90%以上（P ＜ 0.05）。（3）将筛选出的siRNA转染至奶牛乳腺上皮细胞，通过qRT-PCR技术检测乳脂合成相关基因的表达，与对照组相比，干扰ELOVL6基因显著抑制了脂肪酸从头合成及去饱和相关基因乙酰辅酶A羧化酶A（ACACA）、固醇调节元件结合蛋白1（SREBF1）、过氧化物酶体增殖物激活受体γ（PPARG）、硬脂酰辅酶A去饱和酶1（SCD1）、脂肪酸转运相关基因脂肪酸结合蛋白（FABP3）、甘油三酯合成相关基因二酰甘油酰基转移酶（DGAT1）、磷酸甘油酰基转移酶6（AGPAT6）和甘油三酯水解相关基因激素敏感性脂肪酶（HSL）的表达量（P ＜ 0.05）。（4）干扰ELOVL6基因后，乳腺细胞内的甘油三酯含量显著降低（P ＜ 0.05）。上述表明，ELOVL6基因在奶牛乳腺上皮细胞中通过影响脂质代谢相关基因的表达及甘油三酯含量，进而对奶牛乳腺的脂代谢产生调控作用。 [关键词] 超长链脂肪酸延伸酶6|小干扰RNA|乳腺上皮细胞|脂代谢     

The Study on the Cytotoxicity of Palmatine on Goat Endometrial Epithelial Cells in vitro

To explore the cytotoxicity of palmatine in vitro,goat endometrial epithelial cells (EECs) were stimulated by different concentrations of palmatine,then the growth curve of cell was drew by MTT assay which could detect the cell proliferation. The cell growth state was observed by inverted microscope,and Wright-Giemsa staining was used to observe morphological changes of the cells while LDH test was used to check the membrane permeability of EECs. The results showed that when the concentration was 10 to 227 μg/mL,palmatine had a role in promoting proliferation of the EECs,while when the concentration was more than 227 μg/mL,cell proliferation was inhibited,and 50% inhibitory rate of proliferation (IC₅₀) of this drug on EECs was 360 μg/mL.Wright-Giemsa staining results showed that themorphology of the nucleus and cytolymph were not significantly changed when 100 and 200 μg/mL palmatine effected EECs. LDH results showed that when the palmatine concentration was more than 250 μg/mL,LDH content was extremely significantly higher than that of normal cell group(P<0.01).In conclusion,palmatine presented certain toxic effect on EECs with a significant dose-response effect,and the safe dose range of palmatine was less than 250 μg/mL.     

几种熏蒸剂及杀细菌剂对樱桃根癌病菌的室内毒力试验

采用密闭熏蒸法及抑菌圈法测定了氯化苦、棉隆、威百亩、1,3-二氯丙烯及11种杀细菌剂对樱桃根癌病菌的毒力。结果表明:威百亩、棉隆、氯化苦对樱桃根癌病菌的EC50为1.5881、2.7902、5.0475μg/mL,分别为1,3-二氯丙烯的519.60、295.74、163.48倍;杀细菌剂对樱桃根癌病菌的抑菌效果最为明显的是1 000亿活芽孢/g枯草芽孢杆菌可湿性粉剂、72%农用硫酸链霉素可溶性粉剂、85%三氯异氰尿酸可溶性粉剂和46%氢氧化铜水分散粒剂,可应用于田间药效试验进一步验证。     

玉米赤霉烯酮对大鼠子宫内膜细胞Caspase-3和Caspase-8表达的影响

探讨玉米赤霉烯酮(Zearalenone,ZEA)对雌性大鼠子宫内膜细胞凋亡与Caspase-3和Caspase-8蛋白表达的关系,进而揭示其导致子宫损伤的作用机制。应用HE染色观察组织病理变化,免疫组化SP法检测注射玉米赤霉烯酮后6个时间点大鼠子宫内膜细胞Caspase-3和Caspase-8蛋白的表达。病理组织学观察发现,试验组子宫组织出现不同程度损伤,子宫内膜细胞发生凋亡。免疫组化结果显示,试验组子宫内膜上皮细胞与间质细胞Caspase-3和Caspase-8蛋白表达量均明显高于对照组(P0.05)。不同时间点相比,Caspase-3在内膜上皮细胞中12h表达量最高,而在内膜间质细胞中18h表达量最高;Caspase8在内膜上皮细胞中18h表达量达到峰值,而在内膜间质细胞中24h表达量最高,之后缓慢下降。结果表明,ZEA可能通过上调Casepase-3和Caspase-8的表达从而引起子宫内膜细胞凋亡。