Effects of fluid therapy on total protein and its influence on calculated unmeasured anions in the anesthetized dog

Objective – To determine the effects of IV lactated Ringer's solution at a rate of 10 mL/kg/h in anesthetized dogs on total protein (TP) measurement and calculation of unmeasured anions (UAs) using 2 quantitative methods of acid‐base status determination, strong ion gap, and modified base deficit. Design – Prospective clinical study. Animals – Forty‐three dogs, anesthetic health status I or II according to the American Society of Anesthesiologists, undergoing surgery under general anesthesia. Interventions – Arterial blood analyses for gas tensions, acid‐base balance, electrolytes, lactate, hemoglobin (Hb), PCV, and TP were performed under general anesthesia immediately after induction and again after administration of approximately 10 mL/kg of lactated Ringer's solution (given over 1 h). UAs were determined using strong ion gap and modified base deficit. Measurements and Main Results – Fluid replacement for 1 hour decreased TP, Hb, and PCV by 8%, 7.8%, and 8.6%, respectively. The degree of decrease in TP did not impact the calculation of UAs by quantitative methods when the prefluid administration TP value was used instead of the postfluid TP value in the calculation. Comparison of the two methods showed a low correlation (r≤0.68) and marked differences in the precision (1.96 SD). Conclusions: The degree of decrease in TP after 1 hour of fluid replacement at approximately 10 mL/kg does not affect determination of UAs when prefluid TP is used within that time period.     

An analysis of refractometry as a method of determining blood total protein concentration in the American lobster Homarus americanus (Milne Edwards)

Given the high unit value of live American lobsters (Homarus americanus), a non‐destructive field method to assess physiological state is desirable during out‐of‐water transport conditions. This study compared an Uricon® specific gravity refractometer, with a commercial veterinary blood analyser (Vet‐Test), as a method for determining the blood total protein concentration in live, out‐of‐water individuals. Regression analysis of R² = 0.8641 (n = 64) was observed in determination of blood total protein concentration, y = 0.0036x + 1.0163 where x is the protein concentration in mg dL^?1 in Vet‐Test. Our results (R² = 0.864, SE ± 0.003) support the results (R² = 0.985) of the previous study conducted by Leavitt & Bayer (1977 ) in which serum total protein was compared with the refractometric method. We therefore confirm that the refractometric method can be used as a reliable method to indicate health of a lobster by measuring the blood total protein concentration. The results show good correlation between the protein concentrations observed using the refractometric method and a Vet‐Test blood chemistry analyser. anova analysis was significant between the protein refractometer and the Vet‐Test (P < 0.05). Although protein concentration units obtained from both methods were different the results followed the same trends. Refractometry as a method is reliable to determine blood total protein concentrations in the American lobster. Consequently, a refractometric method can be used directly by commercial growers and distributors to assess responses to holding facility conditions and to feeding regimes. Whole blood protein concentrations may provide more information than serum protein concentrations as measured in the study of Leavitt & Bayer (1977 ).     

Pseudo‐hypoproteinemia in a hyperbilirubinemic dog with immune‐mediated hemolytic anemia

A 10‐year‐old spayed female Miniature Poodle was presented to the University of Georgia veterinary teaching hospital for evaluation of lethargy, vomiting and anorexia of 4 days' duration. Physical examination, history and a minimum database led to a diagnosis of immune‐mediated hemolytic anemia accompanied by marked hyperbilirubinemia. Refractometric protein determination was within the reference interval, whereas the biuret method indicated hypoproteinemia. This discrepancy was attributed to interference of bilirubin and biliverdin with the spectrophotometric read‐out of the biuret total protein assay. The albumin concentration, determined by bromcresol green, and refractometric total protein were less affected by this interference.     

微孔滤膜--折光法测定冬笋中可溶性固形物含量

采作微孔滤膜--折光法对冬笋可溶性固形物含量进行测定,同时分别连续测定含量为10%的蔗糖、草酸和柠檬酸水溶液各6次,以验证该方法测定可溶性固形物含量的可行性,结果表明：以该方法测得的蔗糖、草酸、柠檬酸水溶液的平均值分别为9.83%、9.79%、9.71%,其相对标准偏差分别为1.31%、1.04%、1.05%;冬笋中可溶性固形物为6.9%~8.2%,平均含量为7.5%。     

Comparison of an infrared anaesthetic agent analyser (Datex‐Ohmeda) with refractometry for measurement of isoflurane,sevoflurane and desflurane concentrations

ObjectiveTo assess agreement between infrared (IR) analysers and a refractometer for measurements of isoflurane, sevoflurane and desflurane concentrations and to demonstrate the effect of customized calibration of IR analysers.Study designIn vitro experiment.SubjectsSix IR anaesthetic monitors (Datex-Ohmeda) and a single portable refractometer (Riken).MethodsBoth devices were calibrated following the manufacturer’s recommendations. Gas samples were collected at common gas outlets of anaesthesia machines. A range of agent concentrations was produced by stepwise changes in dial settings: isoflurane (0–5% in 0.5% increments), sevoflurane (0–8% in 1% increments), or desflurane (0–18% in 2% increments). Oxygen flow was 2 L minute^?1. The orders of testing IR analysers, agents and dial settings were randomized. Duplicate measurements were performed at each setting. The entire procedure was repeated 24 hours later. Bland–Altman analysis was performed. Measurements on day-1 were used to yield calibration equations (IR measurements as dependent and refractometry measurements as independent variables), which were used to modify the IR measurements on day-2.ResultsBias ± limits of agreement for isoflurane, sevoflurane and desflurane were 0.2 ± 0.3, 0.1 ± 0.4 and 0.7 ± 0.9 volume%, respectively. There were significant linear relationships between differences and means for all agents. The IR analysers became less accurate at higher gas concentrations. After customized calibration, the bias became almost zero and the limits of agreement became narrower.Conclusions and clinical relevanceIf similar IR analysers are used in research studies, they need to be calibrated against a reference method using the agent in question at multiple calibration points overlapping the range of interest.     

Use of refractometry for determination of psittacine plasma protein concentration

Background: Previous studies have demonstrated both poor and good correlation of total protein concentrations in various avian species using refractometry and biuret methodologies. Objectives: The purpose of the current study was to compare these 2 techniques of total protein determination using plasma samples from several psittacine species and to determine the effect of cholesterol and other solutes on refractometry results. Methods: Total protein concentration in heparinized plasma samples without visible lipemia was analyzed by refractometry and an automated biuret method on a dry reagent analyzer (Ortho 250). Cholesterol, glucose, and uric acid concentrations were measured using the same analyzer. Results were compared using Deming regression analysis, Bland–Altman bias plots, and Spearman's rank correlation. Results: Correlation coefficients (r) for total protein results by refractometry and biuret methods were 0.49 in African grey parrots (n=28), 0.77 in Amazon parrots (20), 0.57 in cockatiels (20), 0.73 in cockatoos (36), 0.86 in conures (20), and 0.93 in macaws (38) (P≤.01). Cholesterol concentration, but not glucose or uric acid concentrations, was significantly correlated with total protein concentration obtained by refractometry in Amazon parrots, conures, and macaws (n=25 each, P<.05), and trended towards significance in African grey parrots and cockatoos (P=.06). Conclusions: Refractometry can be used to accurately measure total protein concentration in nonlipemic plasma samples from some psittacine species. Method and species‐specific reference intervals should be used in the interpretation of total protein values.     

Interobserver reliability of canine urine specific gravity assessed by analog or digital refractometers

Refractometry is utilized routinely to evaluate canine urine specific gravity (USG) in veterinary clinical settings. We aimed to determine if the magnitude of interobserver reliability when assessing canine USG via refractometry could impact clinical judgment. USG was determined in 38 dogs by 3 registered veterinary technicians (RVTs) using both an optical analog refractometer and a digital refractometer. Summary statistics were reported, interobserver reliability was assessed via intraclass correlation coefficient (ICC) analysis through a 2-way mixed-effects model, and agreement between RVT pairs was compared through Bland–Altman plots. The median analog refractometer USG measurement was 1.018 (range: 1.004–1.040) and for the digital refractometer was 1.0176 (1.0035–1.0357). The analog refractometer average measure ICC was 0.995 (95% CI: 0.992, 0.997; p < 0.001). The digital refractometer average measure ICC was 0.999 (95% CI: 0.999, 1.000; p < 0.001). Strong agreement between all pairs of RVTs was seen via Bland–Altman plots for both analog and digital refractometers, with 95% CIs spanning no more than 0.002 in either the positive or negative direction for all pairings. The interobserver variability in canine USG measurements by RVTs was trivial and did not impact clinical judgment and decision-making.     

栽培蔬菜与野菜营养物质含量的比较研究

采用分光光度法、折光法、2,6-二氯靛酚滴定法、EDTA滴定法测定了泰安市郊区11种栽培蔬菜和10种野菜中的营养物质含量。结果表明:11种栽培蔬菜中蘑菇的营养价值最高,其次是青椒和菠菜,营养价值较低的是黄瓜;10种野菜中苋菜的营养价值最高,其次是荠菜和香椿,蕨菜的营养价值最低。