全文获取类型
收费全文 | 3188篇 |
免费 | 284篇 |
国内免费 | 370篇 |
专业分类
林业 | 27篇 |
农学 | 127篇 |
基础科学 | 50篇 |
75篇 | |
综合类 | 889篇 |
农作物 | 61篇 |
水产渔业 | 762篇 |
畜牧兽医 | 1769篇 |
园艺 | 29篇 |
植物保护 | 53篇 |
出版年
2024年 | 31篇 |
2023年 | 82篇 |
2022年 | 145篇 |
2021年 | 172篇 |
2020年 | 191篇 |
2019年 | 229篇 |
2018年 | 122篇 |
2017年 | 206篇 |
2016年 | 189篇 |
2015年 | 158篇 |
2014年 | 173篇 |
2013年 | 153篇 |
2012年 | 245篇 |
2011年 | 249篇 |
2010年 | 186篇 |
2009年 | 141篇 |
2008年 | 137篇 |
2007年 | 199篇 |
2006年 | 154篇 |
2005年 | 131篇 |
2004年 | 103篇 |
2003年 | 64篇 |
2002年 | 45篇 |
2001年 | 61篇 |
2000年 | 44篇 |
1999年 | 29篇 |
1998年 | 34篇 |
1997年 | 15篇 |
1996年 | 19篇 |
1995年 | 18篇 |
1994年 | 23篇 |
1993年 | 20篇 |
1992年 | 16篇 |
1991年 | 14篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 5篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1975年 | 1篇 |
1956年 | 5篇 |
排序方式: 共有3842条查询结果,搜索用时 0 毫秒
1.
Perumalraja Kirthika Sungwoo Park Vijayakumar Jawalagatti John Hwa Lee 《Journal of veterinary science (Suw?n-si, Korea)》2022,23(3)
BackgroundProliferative enteritis caused by Lawsonia intracellularis undermines the economic stability of the swine industry worldwide. The development of cost-effective animal models to study the pathophysiology of the disease will help develop strategies to counter this bacterium.ObjectivesThis study focused on establishing a model of gastrointestinal (GI) infection of L. intracellularis in C57BL/6 mice to evaluate the disease progression and lesions of proliferative enteropathy (PE) in murine GI tissue.MethodsWe assessed the murine mucosal and cell-mediated immune responses generated in response to inoculation with L. intracellularis.ResultsThe mice developed characteristic lesions of the disease and shed L. intracellularis in the feces following oral inoculation with 5 × l07 bacteria. An increase in L. intracellularis 16s rRNA and groEL copies in the intestine of infected mice indicated intestinal dissemination of the bacteria. The C57BL/6 mice appeared capable of modulating humoral and cell-mediated immune responses to L. intracellularis infection. Notably, the expression of genes for the vitamin B12 receptor and for secreted and membrane-bound mucins were downregulated in L. intracellularis -infected mice. Furthermore, L. intracellularis colonization of the mouse intestine was confirmed by the immunohistochemistry and western blot analyses.ConclusionsThis is the first study demonstrating the contributions of bacterial chaperonin and host nutrient genes to PE using an immunocompetent mouse model. This mouse infection model may serve as a platform from which to study L. intracellularis infection and develop potential vaccination and therapeutic strategies to treat PE. 相似文献
2.
3.
4.
5.
6.
Rebecca Windsor Samuel Stewart Jessica Schmidt Mario Mosqueda Ignazio Piras Stefan M. Keller Briana Steinmetz Dori L. Borjesson Matthew Huentelman Chand Khanna 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(4):1382
BackgroundNecrotizing meningoencephalitis (NME) in the pug dogs is a fatal neuroinflammatory disease associated with rapid progression and poor response to conventional immunosuppressive therapy. Diagnosis is typically made after severe neurological abnormalities have manifested.Hypothesis/ObjectivePug dogs at genetic risk for NME might manifest neurological abnormalities before developing pathognomonic clinical signs of NME.AnimalsThirty‐six pug dogs less than 4 years of age asymptomatic for NME.MethodsProspective observational cohort study with germline genome‐wide genotyping. Neurological examinations were performed 4 weeks apart to document reproducible findings of central nervous system disease. Magnetic resonance imaging, cerebrospinal fluid analysis, and testing for infectious diseases were performed in all pugs with reproducible abnormalities detected on neurological examination.ResultsThe overall risk allele frequency in this cohort was 40%; 5 (14%) dogs were high risk, 19 (53%) dogs were medium risk, and 12 (33%) dogs were low genetic risk for NME. Reproducible abnormalities detected on neurological examination were identified in 8/24 (33%) genetically at‐risk dogs and 0/12 (0%) low risk dogs. Clinical abnormalities included multifocal spinal pain in 8/8, reduced menace response in 5/8, and lateralizing postural reaction deficits in 5/8 pugs. There was a strong association between genotype risk and the presence of this clinical phenotype (P = .03).Conclusions and Clinical ImportanceOur findings suggest the presence of a novel early clinical phenotype of NME in apparently asymptomatic genetically at‐risk pugs which might be used to plan early diagnostic and therapeutic clinical trials. 相似文献
7.
8.
9.
为研究酵母多糖(YP)对环磷酰胺(CTX)所致免疫损伤大鼠的拮抗作用,本实验将80只雄性SD大鼠随机分为YP(50 mg/kg)+CTX组、YP(100 mg/kg)+CTX组、YP(200 mg/kg)+CTX组、CTX对照组和正常对照组。YP+CTX组按剂量灌胃并称重,CTX和正常对照组则灌胃给予等量生理盐水,连续给药10 d;在第8 d、9 d,除正常对照组外,其余4组腹腔注射CTX 100 mg/kg。第11 d采血及对相关的免疫器官组织进行检测。结果显示,YP(100 mg/kg)组平均日增重和饲料报酬比正常对照组显著增加(p<0.05);胸腺指数,血清中IgA、IgG、表皮生长因子(EGF)、碱性磷酸酶(AKP)含量及空肠SIgA水平显著(p<0.05)或极显著(p<0.01)高于CTX对照组;结肠壁中前列腺素E2(PGE2)含量与CTX对照组相比显著降低(p<0.05)。以上结果表明,YP能提高大鼠的生长性能;YP对CTX所致免疫损伤具有一定的拮抗保护作用,其中100 mg/kg剂量的YP效果最为显著。 相似文献
10.