生产管理中一类排序问题的遗传算法

生产定理系统中经常会遇到一系列排序问题。本文讨论将ｎ个任务安排在ｍ台设备上完成的一类排序问题。对该类排序问题的其他模型进行了进一步讨论,扩展了模型的适用范围,在建立通用模型的基础上,用遗传算法求解。     

猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察

采用Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ／ＰＩ双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒（ＰＲＲＳＶ）实验感染ＳＰＦ猪不同时期外周血单核细胞和肺泡巨噬细胞感染Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群（早期凋亡细胞群）。结果显示,ＰＲＲＳＶ感染猪外周血单核细胞和肺泡巨噬细胞Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群的表达率均明显高于正常对照猪,感染后２４ｈ表达率达最高值。     

Expression profiling of transgenes (Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds

Transgenic cotton carrying the CrylAc gene has revolutionized insect pest control since its adoption,although the development of resistance in insect pests has reduced its efficacy.After 10 years of cultivating Bacillus thuringiensis(Bt) cotton with a single Cry1 Ac gene,growers are on the verge of adopting Bt cotton that carries the double gene(Cry1 Ac+Cry2 A) due to its better effectiveness against insect pests.Thus,the current study was designed to evaluate the role of each gene in the effect...     

番鸭血浆酶活性及其与产肉性能关系的研究

用法国＊本地二元杂交鸭为研究对象,分析其宰前血浆ＧＯＴ、ＧＰＴ、Ａｋｐ、ｒ－ＧＴ、Ａｍｙ活性与７个产肉性状的表型相关,结果表明ＧＯＴ活性与屠宰率,胸肌率,腿肌率,观众 率,ＧＰＴ活性与胸肌率,瘦肉率、ｒ－ＧＴ活性与胸肌率,腹脂率均呈显著正相关;     

Effects of different forms and origins of oilseeds on dynamics of ruminal biohydrogenation of long‐chain fatty acids in vitro

Dietary unsaturated fatty acids (FA) are intensively hydrogenated in the rumen, resulting in reduced amount of poly‐unsaturated fatty acids (PUFA) and accumulation of several biohydrogenation (BH) products. In this study, BH of PUFA originating from different oilseeds (linseed, soya beans, sunflower seed and rapeseed) present in crushed oilseeds or their free oils were assessed in vitro. The assay substrates were incubated in buffered rumen fluid for 0, 6, 12 and 24 h. After incubation, the FA pattern of the incubated samples was analysed using gas chromatography. Biohydrogenation is defined as disappearance of double bonds (DB) calculated from the contents of unsaturated FA. After 24‐h incubation, the DB contents of all oilseeds were reduced (p < 0.001) by 40–60%. The reduction was higher (p < 0.001) for the crushed form compared with the oil form. In addition, linseed and sunflower seed known as oilseeds with high contents of linolenic acid C18:3 c9,12,15 (LNA) and linoleic acid C18:2 c9,12 (LA), respectively, showed a higher (p < 0.001) accumulation of the BH intermediates conjugated linoleic acid (CLA, isomer C18:2 c9t11) and vaccenic acid (C18:1 t11) for the crushed form, when compared with the oil. These results suggest an inherent effect of the physical form of the assay oilseeds on in vitro BH. Changes in FA pattern during BH in vitro can be attributed to both source and physical form of the assay oilseeds. However, further investigations are warranted to ensure whether the observed in vitro effects on ruminal BH can be confirmed in vivo.     

珍禽贵妃鸡自别雌雄系的选育——二世代慢羽公鸡的测交试验

针对贵妃鸡在国内外珍禽市场上不可估量的发展前景,为了选育出贵妃鸡自别雌雄配套系,本研究根据伴性遗传基因的遗传原理和羽速基因的遗传特点,采用现代家禽育种方法对贵妃鸡第二世代表型慢羽公鸡进行了测交试验。采用人工授精,所产种蛋分别孵化。其中用13只表型为慢羽公鸡,与52只快羽母鸡组成第1类测交试验,结果每只公鸡的后代中都有快羽雏出现,且快慢羽比例几乎为1:1(217:232);由20只表型慢羽公鸡与80只慢羽母鸡组成的第2类测交试验,结果每只公鸡的后代中,也都有快羽雏出现,而且快慢羽比例几乎接近1:3(197:520)。可见,所用的33只公鸡经测交证明全为杂合体,同时表明两个不同质量性状测交结果完全符合孟德尔遗传定律。     

鸡白细胞介素-6原核表达及双抗夹心ELISA方法的建立

应用分子生物学技术原核表达ChIL-6,以ChIL-6重组蛋白为免疫原,按免疫程序分别制备兔抗和鼠抗IL-6重组蛋白的多克隆抗体。应用此抗体建立双抗夹心EL-ISA方法后,为了优化此方法本试验对该方法的最佳试验条件、标准曲线、重复性和初步应用进行确定。结果显示,经SDS-PAGE和Western-blot分析,表明ChIL-6在大肠杆菌中正确表达,为了建立检测ChIL6的双抗夹心ELISA方法,本试验应用表达的重组蛋白制备兔抗和鼠抗多克隆抗体。建立的双抗夹心ELISA方法最佳反应条件为包被抗体的质量浓度为50mg／L,4℃过夜;酶标二抗稀释度为1：400,37。C1h;应用该方法检测感染金黄色葡萄球菌后的ChIL-6,其结果与本实验室之前应用人的ELISA试剂盒检测的结果相似。结果表明,本试验建立的检测ChlL-6的双抗夹心ELISA方法可用于临床。     

金陵麻鸡选育及其配套模式杂种优势分析

以华青麻鸡、良凤花鸡和隐性白羽肉鸡作为育种素材,经过4～5个世代的连续选育形成3个专门化品系(M系、A系和R系).根据生产需要和品系特征设计不同的杂交组合,通过配合力测定和重要性状杂种优势率分析,筛选出最佳配套组合M♂×(A×R)♀.配套形成的金陵麻鸡商品代公、母鸡出栏日龄为56 d,体重分别为1 943 g和1 554 g,饲料转化率分别为2.26:1和2.24:1,成活率分别为98.3%和97.5%.     

南疆绒山羊的培育

南疆白绒山羊是通过对当地山羊的选育和杂交改良，经过近20年的努力育成的。通过本品种选育，横交固定，饲养管理等技术的实施，培育出了体型外貌一致，生产性能高，遗传性能稳定的新品种白绒山羊7．6万余只，并且建成年生产种羊达1000只的种羊生产基地，采取边培育边推广的方法，使阿克苏地区绒山羊平均个体产绒量达175．6g，取得了明显的经济，社会效益。     

Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs

There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized only AHSV-3. In contrast, the one selected with AHSV-8 captured by IgG against AHSV-3 recognized all nine AHSV serotypes but not the Bryanston strain of equine encephalosis virus. Serving as evidence for its serogroup-specificity. These two scFvs can help to rapidly confirm the presence of AHSV while additional serotype-specific scFvs may simplify AHSV serotyping.