The effect of some Frescon analogs on the aquatic snail,Lymnaea stagnalis: II. Correlation of neurotoxicity to Molluscicidal effectiveness

Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties.     

甜瓜新品种-中甜1号的选育

中甜1号为优质、高产、耐湿、早熟厚皮甜瓜品种,果实发育期25-28d,果实椭圆形,果皮黄色,上有10条银白色纵沟,果肉白色,单瓜重1.0-1.5kg,可溶性固形物含量13.5%-15.5%。适应性广,在保护地、露地条件下均可栽培。     

珍黄88(湘椒29号)辣椒的选育

珍黄88的母本6427是从广西地方品种经自交纯化而成的优良黄皮尖椒自交系;父本8215为叶少、部分茎节短缩、肉厚疏松的辣椒高代自交系。该品种中熟,定植至采收青椒约50 d(天),产量2500～2800 kg·(667 m~2)~(-1),抗病毒病,中抗疫病,适于山东、广东等黄皮尖椒外运基地栽培;果实长牛角形,果表黄绿无皱,果型直,肉厚腔小,商品性好,耐贮运。     

Sca-1+ cells from mouse fetal liver can differentiate into neurons in vitro

AIM: To study whether Sca-1⁺ cells from fetal liver can be induced to differentiate into neuronal cells in vitro. METHODS:Sca-1⁺cells from 14 5-days-old murine fetal liver were isolated with a magnetic cell sorting kit, and were cultured in Dulbecco s modif ied Eagle s medium(DMEM)/F12 supplemented with 10%fetal bovine serum(FBS), and passaged at a rat io of 1 3 when cells reached more than 80%confluence.The 5 passage cells were induced by 10^-3mol/Lβ-mercaptoethanol(β-ME)and 5×10^-7 mol/L all-trans-retinoic acid(RA)for 24 hours, and then incubated in serum-free medium for 5 hours to 5 days.The characteristics of treated cel s were assayed by immunocytochemistry staining analysis at 5 hours, or 5 days.RESULTS: Cells treated with β-ME and RA exhibited neuronal phenotype and expressed neuron-specific protein such as neuron-specific nuclear protein (NeuN), neuronfilament-M, and neuron-specific tubulin-1 (TuJ-1) but not tau, MAP-2, or the astrocyte-specific marker glial fibrillary acidic protein (GFAP).CONCLUSION: Sca-1⁺ cells from fetal liver, of which most are regarded as hematopoietic stem cells, could differentiate into early immature neuronal cells in vitro. These findings suggest that Sca-1⁺ cells from fetal liver may be an alternative source in cell therapy and gene therapy of neural dysfunction.     

干椒新品种蓉椒6号的选育

自交系9804和9909杂交配制成杂交一代干椒，植株生长旺盛，座果力强，较抗病毒病和疫病，果实指形，纵长17～19cm，横径1．2cm，青熟果绿色，老熟果鲜红色，单果重8．3g，味辣，品质佳，中熟品种，从定植到采收55d(天)，一般每667m^2产1500～1900kg。     

吡啶衍生物研究(IX):N-(1-甲氧羰基)乙基-N-[5-(2-氯吡啶-4-基)-1,3,4-噻二唑-2-基]酰胺的合成及除草活性

为了进一步研究前期发现的除草先导化合物2-仲丁氨基-5-(2-氯吡啶-4-基)-1，3，4-噻二唑(BCPT)的结构-活性关系并提高其除草活性，设计并合成了一系列N-(1-甲氧羰基)乙基-N-[5-(2-氯吡啶-4-基)-1，3，4-噻二唑-2-基]酰胺类化合物。其苗后除草活性测定结果表明，所有化合物的活性都远低于BCPT本身。说明BCPT可能具有与传统酰胺类除草剂不同的作用机制。     

披碱草和野大麦杂种F1与BC1F1代的生物学及农艺特性研究

对披碱草和野大麦及其杂种F1与BC1F1代的生物学及农艺特性进行了分析比较。结果表明,亲本野大麦生长发育节律较快,较早地开花结实,果后营养期较长,生育期74d,生长天数达206d,具有很强的分蘖能力;披碱草生长发育节律较慢,生育期124d,生长天数193d;杂种F1的生长天数介于双亲之间,生长动态偏向亲本披碱草,分蘖能力介于双亲之间;BC1F1代生长发育节律较F1代提早,不同株系生长天数不同,生长动态偏向轮回亲本野大麦,分蘖能力有较大的变异。杂种F1代表现出较强的杂种优势,BC1F1代产草量较F1代有所降低,不同株系间存在明显的差异。总体上,BC1F1代的生产性能倾向于轮回亲本野大麦。     

四川地区猕猴桃病毒1的RT-PCR检测及外壳蛋白基因序列分析

为了明确近期新西兰报道的侵染猕猴桃的新病毒——猕猴桃病毒1(Actinidia virus 1,AcV-1)在四川地区猕猴桃上的发生情况及其分子特性,采用RT-PCR对来自四川6个地区疑似感染病毒的90份猕猴桃主栽品种‘红阳’和‘金果’的叶片样品进行检测。结果表明,22份样品为AcV-1阳性,检出率为24.4%。将获得的5个AcV-1分离物和已报道的新西兰分离物K75的外壳蛋白(coatprotein,CP)基因序列进行比对,结果表明,分离物间核苷酸序列和氨基酸序列的相似性分别为84.8%～97.1%和89.7%～99.6%,其中除邛崃分离物HYH5与新西兰分离物K75的核苷酸序列相似性较高(97.1%)外,其余4个分离物均低于91%。系统进化分析结果显示,这些分离物主要聚集在3个分支上,分离物HYH5与新西兰分离物K75位于同一分支,其余分离物位于另外2个分支。     

枸杞Lb＿PPR1的克隆及其在不同育性品种中的表达分析

三角状五肽重复(Pentatricopeptide repeats,PPR)蛋白定位于多种细胞器中,参与细胞核和细胞器中特异单链RNA的转录后修饰和编辑,在植物生长发育的多个阶段均发挥着重要的作用。分别从枸杞(Lycium barbarum)雄性可育系‘宁杞1号’和不育系‘宁杞5号’中克隆了Lb_PPR1基因,并对其编码蛋白质进行理化性质、亚细胞定位、保守结构域、蛋白结构以及系统进化等方面进行预测分析。结果显示,‘宁杞1号’和‘宁杞5号’中Lb_PPR1基因的开放阅读框均为1 977 bp,编码658个氨基酸,但其中存在20个碱基和14个氨基酸的差异。Lb_PPR1蛋白包含14个串联重复的PPR保守基序,属于PLS家族,该蛋白定位在细胞膜和细胞质。二级、三级结构预测显示该蛋白以α螺旋为主。同源进化分析得出Lb_PPR1蛋白与同属茄科的辣椒和烟草PPR蛋白亲缘关系最近。利用实时荧光定量PCR检测Lb_PPR1在枸杞不同组织器官及不同发育阶段花药中的表达特性。结果显示,Lb_P