Analysis of gene expression profile of multidrug resistant MCF/DOX cell line after benflumetol derivative LY980503 treatment

AIM:To investigate the effect of LY980503(a benflumetol derivative)on multidrug resistance of tumor cell line using DNA microarray.METHODS:Total RNA was extracted from multidrug resistant MCF/DOX cell line. cDNA microarray containing 320 cDNAs was used to detect the gene expression profile.RESULTS:9 down-regulated genes and 1 up-regulated gene were identified after multidrug resistant MCF/DOX cells were treated with LY980503.CONCLUSION:LY980503 can effectively reverse the resistance of MCF/DOX to DOX in vitro by adjusting the expression of multi-genes.     

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.     

Effect of antifibrotic herb serum on rat hepatic stellate cell proliferation and collagen synthesis

AIM: To investigate the effect of Chinese herbs, Ganxianfang(GXF), on rat hepatic stellate cells (HSC) proliferation and collagen synthesis. METHODS: Two types of herb serum, portal venous serum and circumferential venous serum, were prepared from rats infused intragastrically with 16, 8, 4 times adult dose of GXF decoction. HSC isolated from rat liver were processed with the above sera in vitro. Then we mensurated the radioactivity of HSC admixed with [[³H]H]proline and [[³H]H]thymine to judge the effect on proliferation and collagen synthesis of HSC. RESULTS: Both two types of serum collected 0.5, 1, 2 h after intragastrical infusion inhibited HSC proliferation (P＜0.05), and the serum collected 1 h after intragastrical infusion had the strongest effect (P＜0.05). Portal serum decreasea collagen synthesis (P＜0.05), but circumferential serum had no effect (P＞0.05). CONCLUSION: Inhibition of HSC proliferation and decrease of collagen synthesis may contribute to the GXF antifibrotic action.     

黄河鲤鱼源维氏气单胞菌的分离·鉴定及药敏试验

[目的]为研究鲤鱼感染细菌性疾病及其临床合理用药提供理论依据.[方法]从吉林省某水产养殖场患病黄河鲤鱼的脾脏中分离致病菌,并通过常规生理生化试验、16S rDNA序列分析对其进行鉴定.应用微量稀释法测定11种抗生素药物对其最小抑菌浓度.[结果]从患病鲤鱼的脾脏中成功分离到1株优势菌.该优势菌为革兰氏染色阴性杆菌,通过序列比对将其鉴定为维氏气单胞菌.动物回归试验表明该菌株可使黄河鲤发生死亡.药敏试验结果表明该病原菌对磺胺类、β-内酰胺类、氨基糖苷类药物不同程度耐药,而对氯霉素类药物、恩诺沙星较为敏感,对强力霉素中介.[结论]该研究结果可为鲤鱼源维氏气单胞菌的防治提供科学依据.     

中国和欧盟动物源性产品中药物残留限量差异性分析(一)

将中国和欧盟动物源性产品中药物残留标准进行了对比,同时结合TBT/SPS预警数据库,找出二者差异性,并提出了改进建议。     

关于兽用抗菌药使用减量化行动的探索与实践

宁夏以兽药生产经营使用环节全链条监管、动物源细菌耐药性监测技术体系建立、养殖场生物安全体系构建、减抗替抗产品推广应用、示范点带动等重点工作为切入点,强化质量保障,重视创新驱动,构建“政府+企业”产学研合作机制,不断促进兽用抗菌药使用减量化,形成了一套“监管到位、技术保障、企业尽责、用药减量”的兽用抗菌药使用减量化技术推广模式。     

Reversing effect of naringin on cisplatin resistance in human lung cancer A549/DDP cells

AIM: To investigate the effect of naringin (NRG) on cisplatin (DDP) resistance in human lung cancer A549/DDP cells and its possible mechanism. METHODS: A549/DDP cells were cultured in vitro and treated with NRG and/or DDP at different concentrations for 24 h, and then the cell viability were measured by CCK-8 assay. The combination index (CI) of NRG and DDP were analyzed by Chou-Talalay method. The apoptosis rate was analyzed by flow cytometry. Western blot was performed to detect the protein levels of P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1), p-Akt, CXC chemokine receptor 4 (CXCR4), cleaved caspase-3, Bcl-2 and Bax.RESULTS: The protein levels of P-gp, MRP1, p-Akt and CXCR4 in the A549/DDP cells were higher than those in the A549 cells (P<0.05). The cell viability was remarkably reduced in a dose-dependent manner when A549/DDP cells were exposed to NRG and/or DDP (P<0.05), and the IC₅₀ values of NRG and DDP were 36.92 μmol/L and 129.77 μmol/L, respectively. When the inhibition rate exceeded 15%, NRG in combination with DDP produced a synergistic effect (CI<1). Combination treatment with NRG and DDP significantly induced apoptosis (P<0.05), up-regulated the protein levels of cleaved caspase-3 and Bax, and down-regulated the protein level of Bcl-2 (P<0.05). Meanwhile, NRG remarkably down-regulated the protein levels of P-gp, MRP1, p-Akt and CXCR4 in a dose-dependent manner (P<0.05). CONCLUSION: NRG may enhance the sensibility of A549/DDP cells to DDP most likely via up-regulating the protein level of Bax and down-regulating the protein levels of Bcl-2, P-gp, MRP1, p-Akt and CXCR4.     

副猪嗜血杆菌病病原的分离鉴定与药敏试验

对1例临床、病理剖检症状疑似副猪嗜血杆菌病进行实验室诊断,最后确诊其病原为副猪嗜血杆菌。经药敏试验显示,副猪嗜血杆菌对氨苄青霉素、卡那霉素、庆大霉素等药物敏感。     

江苏地区小麦赤霉病菌种群检测与抗药性分析

本研究旨在检测江苏地区小麦赤霉病菌的种群组成与对传统杀菌剂单剂的抗性严重度。以江苏溧阳、通州和盐城地区小麦赤霉病菌子囊孢子为试材,鉴定其优势种群,检测对多菌灵、戊唑醇和咪鲜胺的抗性。结果表明：江苏地区小麦赤霉病菌株中全部检测到亚细亚镰孢,是绝对优势种群;禾谷镰孢占比最高是溧阳地区的14%,最低是通州地区的8%,平均占比为11.33%,显著低于亚细亚镰孢占比;江苏地区小麦赤霉病菌对多菌灵的抗性频率介于26.3%~54.5%;未检测到戊唑醇和咪鲜胺具有抗性的赤霉菌株。综上,江苏地区小麦赤霉病菌优势种群是亚细亚镰孢,其对多菌灵单剂已产生严重抗性,未发现对戊唑醇和咪鲜胺产生抗性。     

毛细管区带电泳在药物分析中的研究进展

近年来,有关毛细管区带电泳在药物领域中的相关研究骤增。综述了手性拆分中常用的环糊精类化合物手性选择剂的发展及其应用;同时对毛细管电泳在手性药物分析中的应用进行了展望。