Effect of Liucha extract on tumor K562 cell growth

AIM:To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism.METHODS:The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope.RESULTS:Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared.CONCLUSION:The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells.     

Effects of aqueous extracts and decomposition of Mikania micrantha H.B.K. debris on selected agronomic crops

Laboratory and greenhouse experiments were conducted to determine the effects of aqueous extract and the debris of Mile-a-Minute ( Mikania micrantha H.B.K.) on the germination and growth of four test species, viz. tomato ( Lycopersicon esculentum Mill.), Chinese cabbage ( Brassica chinensis L.), corn ( Zea mays L.) and long bean ( Vigna sesquipedalis L.). Germination percentage, radicle length and fresh weight of two crops, Chinese cabbage and tomato, decreased progressively when plants were exposed to increasing concentrations (12.5, 25, 37.5 and 50 g L⁻¹) of aqueous extract of Mikania , but did not affect those of corn and long bean seedlings. The emergence of tomato and Chinese cabbage was greatly reduced when Mikania debris was incorporated into the soil, irrespective of the amount of debris and the decomposition period. In contrast, the emergence of corn and long bean seedlings was not affected. The application of fertilizer (NPK 15 : 15 : 15) enhanced the fresh weight of all seedlings tested as compared with those not supplied with the fertilizer. Four compounds were identified in the leaf extract of Mikania , viz. caffeic acid, p -hydroxybenzaldehyde, resorcinol and vanillic acid.     

黄花蒿粗提物对几种害虫拒食性的初步研究

以黄花镐为原料进行浸提，经生物活性测定，结果表明黄花蒿Artenisia annua L.粗提物对供试的6种害虫均具有拒食性。其中对黑翅土白蚁Odontotermes formosanus Shiraki、赤拟谷盗Thibolium castaneum Herbst、谷蠹Rhizopertha dominica Fabricius拒食性极强，对棉蚜Aphis gossypii Glover、棉红蜘蛛Tetranychus urticae Koch及豇豆荚螟Etiella zinckenella Treitschke 也具有较强的巨食性。使用黄花蒿粗提物时，以稀释500倍和800倍效果最好，处理与对照之间有极显著差异。     

丝兰属植物提取物降低鸡舍氨气浓度的效果试验

通过在21日龄的肉用仔鸡饲料中添加丝兰属植物提取物(其有效成分为丝兰皂角苷)，研究丝兰属植物提取物对降低鸡舍中氨气浓度和提高肉仔鸡生产性能的效果。试验结果表明，试验组鸡舍内氨气平均浓度为4．75mg／L，对照组鸡舍内氨气平均浓度为13．80mgl／L，试验组比对照组鸡舍内氨气平均浓度降低了9．05mg／L，经t检验，两鸡舍内氨气平均浓度差异显著(P＜0．05)，并且试验组比对照组饲料报酬提高了8.92％。     

富铁酵母对家兔红细胞免疫功能的影响

为探讨富铁酵母对家兔红细胞免疫功能的影响,将32只家免随机分成富铁酵母高、低剂量组,FeSO4组及空白对照组,分别灌服富铁酵母(Fe元素8 mg/kg、4 mg/kg)、FeSO4(Fe元素4 mg/kg)及空白生理盐水;连续5 d后,检测红细胞C3g受体花环率和复合物(IC)花环率.结果:富铁酵母较FeSO4及空白组能显著提高红细胞C3h受体花环和红细胞复合物(IC)花环的百分率,但高、低剂量间无显著性差异.结论:富铁酵母能显著提高红细胞免疫功能.     

酵母铬与L-肉碱对蛋鸡生产性能及脂质代谢的影响

选用21周龄海兰褐壳蛋鸡288只,随机分为9组,每组32只,采用3×3(铬×L-肉碱)2因子3水平有重复交叉试验设计,预试2周后,在玉米-豆粕型日粮的基础上添加不同水平的酵母铬(以铬计:0、400、600μg/kg)与L-肉碱(0、50、100mg/kg),以饲喂基础日粮组为对照组,用以研究二者对蛋鸡生产性能及脂质代谢的影响,并初步探讨二者的互作效应,试验期7周。结果表明:600μg/kg铬或100mg/kgL-肉碱对脂质代谢多数指标效果显著(P<0.05或0.01);二者同时添加对蛋黄胆固醇、肝脏甘油三酯及腹脂率等均产生显著互作效应(P=0.0003～0.0500),且400μg/kg铬和100mg/kgL-肉碱混合添加对降低蛋黄胆固醇比其它各组均表现出优势,400μg/kg铬和50mg/kgL-肉碱混合添加对降低腹脂率比其它各组均表现出优势。铬或(与)L-肉碱在降低蛋鸡肝脂、腹脂及蛋黄胆固醇的同时并未影响蛋鸡的生产性能。     

添加不同酵母培养物对瘤胃纤维分解菌群和纤维素酶活的影响

用3种酵母培养物(YC- 1、YC 2和YC- 3)分别饲喂4头带有永久性瘤胃瘘管的肉牛,研究培养物对瘤胃发酵、纤维分解酶活性和3种纤维分解菌数量的影响,结果表明:YC 2处理的乙酸、丙酸、丁酸和总 VFA浓度显著高于对照组(P<0 .05),YC 1和YC 3处理的乙酸/丙酸比例显著降低(P<0 .01);各处理均能显著提高瘤胃内羧甲基纤维素酶、水杨苷酶和木聚糖酶的活性(P<0 .01);各处理都显著提高黄化瘤胃球菌的相对比例(P<0 .01),16SrRNA特异性寡聚核苷酸探针杂交法分析测定结果表明 3 种纤维分解菌在瘤胃细菌中所占比例为 3. 80%±0 .2%。     

人参提取液体外抑制IHNV作用效果研究

探究人参提取液体外抑制CCB细胞感染IHNV的能力,构建体外CCB细胞模型,运用RTCA技术和MTT法测得不同浓度人参提取液对IHNV抑制作用曲线,并从阻断病毒复制和杀灭病毒两方面讨论人参提取液作用效果。结果表明,4%人参提取液对IHNV具有明显抑制作用,能够在16 h内保护细胞不受IHNV病毒的侵染,其中2 h时对病毒灭活抑制率最高达到51.5%,1~12 h内对病毒复制有持续抑制作用。研究可为IHVN防治药物的研发提供新的思路及参考。