Supplemental iron and phosphorus increase dietary inclusion of cottonseed and soybean meal in olive flounder (Paralichthys olivaceus)

A long‐term feeding experiment was conducted to investigate the use of cottonseed and soybean meal (CS) with iron and phosphorus supplements in diets for olive flounder (Paralichthys olivaceus). Olive flounder with an initial average size of 28.5 ± 0.35 g (mean ± SD) were divided into 15 groups (three tanks per dietary treatment) and fed 480 g kg^?1 crude protein diets in which each of five isonitrogenous diets was formulated to contain different levels of cottonseed/soybean meal (1 : 1) to replace fish meal (FM) with iron and phosphorus supplementations. The five experimental diets were as follows: diet 1 (control), 0%CS; diet 2, 20%CS; diet 3, 30%CS; diet 4, 30%CS + Fe&P; and diet 5, 40%CS + Fe&P. After 26 weeks of feeding trial, no significant differences were observed in weight gain, feed utilization and survival among all the treatments. The total gossypol accumulation in liver of fish fed diets supplemented with iron was significantly lower than that of fish fed diets without supplementation of iron. The results indicate that the addition of iron in diets could prevent the absorption of free gossypol. The findings in this study suggest that dietary supplements of iron and phosphorus could increase the inclusion of cottonseed and soybean meal for FM replacement in diets for marine fish species.     

Susceptibility by amberjack (Seriola dumerili), yellowtail (S. quinqueradiata) and Japanese flounder (Paralichthys olivaceus) to Neobenedenia girellae (Monogenea) infection and their acquired protection

This study investigated: 1) susceptibility differences to infection by Neobenedenia girellae (Capsalidae) between amberjack Seriola dumerili (Carangidae), yellowtail S. quinqueradiata and Japanese flounder Paralichthys olivaceus (Paralichthyidae); 2) growth and egg production of N. girellae on each fish species; 3) acquired protection of each fish species against this parasite. The number of N. girellae on S. dumerili was significantly higher than on S. quinqueradiata and P. olivaceus when these fishes were exposed to oncomiracidia in the same aquarium. Neobenedenia girellae growth on S. dumerili was fastest and, thus the number of eggs laid by parasites on S. dumerili was greater than on the other two species. Seriola dumerili and P. olivaceus, which were previously infected with N. girellae and treated by freshwater bath, acquired partial protection against re-infection by N. girellae. The relative re-infection of three S. dumerili individuals out of eleven individuals was markedly low compared with the initial infection, and the relative initial infection and re-infection on two P. olivaceus out of eleven individuals was markedly low. The results of this study could be useful to control N. girellae infections when cultivating S. dumerili, S. quinqueradiata and P. olivaceus.     

Immobilization antigen-independent protection of olive flounder (Paralichthys olivaceus) against Philasterides dicentrarchi (Ciliophora: Scuticociliatia) infection

To determine whether olive flounder (Paralichthys olivaceus) immunized with lysate of Philasterides dicentrarchi could be protected immobilization antigen (i-antigen) type independently, fish were immunized with lysate of ciliates obtained from in vitro cultures or ciliates obtained from infected fish. Fish immunized with ciliates obtained from infected fish were completely protected against artificial infection with in vitro cultured P. dicentrarchi, in spite of a weak or no serum agglutination activity against in vitro cultured ciliates. The present results indicate that i-antigen-independent protection was elicited by immunization of fish with the ciliates lysate, and the cultured ciliates would provide a good source for preparation of vaccines, which are cross-protective against various i-antigen types of P. dicentrarchi.     

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.     

间型鸡(从)(Termitomyces intermedius):一个分布于南亚热带至温带南缘的物种

分别对2016年采自河南省信阳市和2017年采自广东省广州市的部分鸡(从)(Termitomyces spp.)标本进行形态学鉴定和基于ITS序列的分子系统学分析.发现了一个中国新记录种——间型鸡(从)(T.intermedius),它可分布于南亚热带至温带南缘,比2016年在日本冲绳石垣岛发现的分布区域更靠北端.同时发现河南省温带南缘有小鸡(从)(T.microcarpus)分布,是已报道的中国鸡(从)分布区域的最北端.     

桂南地区杉木马尾松人工实生林多形地位指数曲线模型

利用桂南地区收集的人工实生林分标准地材料和优势解析木杉木９３株，马尾松１６２株，建立优势高生长Ｒｉｃｈａｒｄｓ模型。用参数预估法编制桂南地区的杉木、马尾松实生林多形地位指数表。杉木、马尾松各指数级曲线拟合平均剩余标准差分别是０．２９４３ｍ和０．２３２３ｍ；经另外６８株和５２株优势解析木检验，预报误差分别为０．４８７ｍ和０．５０６ｍ，适应性好。     

广西贺州地区温室内空气湿度时空变化研究

以温室为研究对象,探讨研究了晴天、阴天、雨天3种不同类型天气条件下温室内空气相对湿度与绝对湿度的日周期时空变化规律以及整个冬季空气相对湿度与绝对湿度的时空变化规律。为合理调控温室内空气湿度以控制作物病虫害、提高产量和改善品质提供科学依据。     

牙鲆养殖循环系统中固体废物的去除研究

对自行设计的牙鲆Paralichthys olivaceus循环养殖系统采用斜板式沉淀槽、水力旋流器、泡沫分离器去除固体颗粒物的效果进行了研究。结果表明:该系统实际运行过程中,养殖槽的固体颗粒物含量小于10 mg/L;对养殖水中总悬浮固体颗粒物的去除能力,沉淀槽最强,去除量(干重,下同)为(11.3±3.5)g/d,占69.2%,水力旋流器为(4.8±1.0)g/d,占29.5%,泡沫分离器为(0.2±0.1)g/d,占1.3%;对难沉降颗粒物的去除能力,泡沫分离器较强,去除量为(4.2±1.1)g/d,占53.1%,其中挥发性悬浮固体占93.2%,沉淀槽为(3.6±0.7)g/d,占44.8%,水力旋流器为(0.2±0.0)g/d,占2.1%;牙鲆摄食饲料所产生的固体废物量(干重)约为245.8 g/kg。     

宁夏南部山区不同土地类型土壤养分的分布特征研究

通过对宁夏南部山区阳洼流域不同土地类型下土壤养分分布特征进行研究,结果表明:流域土壤养分整体上表现为草地>农地>梯田>林地>沟道.流域各类型下土壤养分随土层深度增加,有逐渐下降的趋势.在α=0.05下,不同土层间土壤速效磷、速效钾、全磷存在显著差异.在不同土地类型下土壤全磷受母质影响,变异系数仅为21.41%,在土壤中以稳定态存在,而流域有效磷的变异系数可达178.56%,土壤有效磷含量的空间分布较为复杂,易受随机因素影响.草地利用类型下土壤有机质、碱解氮的变异系数为35.24%,29.04%,远高于林地利用类型.pH值与速效磷、全氮、有机质和碱解氮都有极显著的相关关系,有机质和碱解氮、全氮相关性较高,相关系数(R)分别为0.914**,0.839**,而全磷与有效磷之间没有表现出明显相关关系.