Life cycle of Caligus rogercresseyi, (Copepoda: Caligidae) parasite of Chilean reared salmonids

Caligus rogercresseyi, [Contrib. Zool. 69 (2000) 137] is the only caligid known to affect the salmon industry in Southern Chile. Economic losses due to reduced fish quality, cost of chemical treatment and outbreaks of other diseases such as the Piscirickettsiosis occur. The life cycle of C. rogercresseyi is described in rainbow trout reared in seawater tanks from observations made under natural conditions of light and temperature between January 1997 and April 1998. Fish were infected with laboratory-cultured larvae obtained from ovigerous females. Rainbow trout were periodically slaughtered for parasite collection and identification. C. rogercresseyi life cycle includes the following stages: two nauplius, one copepodid, four chalimus and the adult. No preadult stage was observed. Timing of the different stages of development was directly dependent on water temperature. The maturation of the eggs or the time for a complete life cycle took place at 45 days in July at 10.3 °C, 31–32 days in April at 12.4 and 12.8 °C, respectively, and at 26 days in November at 15.2 °C. In January, at 16.7 °C, only the appearance of first eggs were observed at 18 days. A simple degree–day (dd) model is proposed for each developmental stage between 4 and 17 °C, where the development rate is a linear function of the average temperature of water. Using this degree–day model, the proportion of fourth stage chalimus was maximum at 172 dd of effective temperature, adult males at 193 degree–days, adult females at 208 dd. The minimum temperature threshold is at 4.2 °C where there is no development of the parasite. The appearance of first eggs occurred at 231 dd and the first pigmented eggs at 277 dd. The temperature-independent degree–days value allowed to predict the timing of C. rogercresseyi life cycle at any temperature within the evaluated range.     

Pathogenicity of white spot syndrome virus on postlarvae and juveniles of Penaeus (Litopenaeus) vannamei

White Spot Syndrome Virus (WSSV) has decimated the shrimp aquaculture around the world. Breeding efforts to generate resistant stocks are necessary but there is a lack of basic information on challenge test strategies focused on genetic selection. Infection routes and developmental stages were evaluated on Penaeus vannamei as a first step in a strategy to select white spot virus (WSSV)-resistant stocks. Mortalities could not be induced before the PL30 stage. The impact of infection by immersion and blended tissue was intermediate on mortalities when compared to the minced tissue treatment on PL30. Blended and minced tissue treatments produced the highest mortalities on PL40 while immersion was intermediate. A general tendency towards higher susceptibility associated with older stages was detected. Additionally, juveniles of 1 g average weight from three local breeding programs were challenged. There were no differences in survival between the programs, although two of them derived their progenies from survivors of strong WSSV events. The implications of these results to the WSSV epidemiological characteristics and breeding programs are discussed.     

QTLs for Waterlogging Tolerance at Germination and Seedling Stages in Population of Recombinant Inbred Lines Derived from a Cross Between Synthetic and Cultivated Wheat Genotypes

Waterlogging is a widespread limiting factor for wheat production throughout the world. To identify quantitative trait loci （QTLs） associated with waterlogging tolerance at early stages of growth, survival rate （SR）, germination rate index （GRI）, leaf chlorophyll content index （CCI）, root length index （RLI）, plant height index （PHI）, root dry weight index （RDWI）, shoot dry weight index （SDWI）, and total dry weight index （DWI） were assessed using the International Triticeae Mapping Initiative （ITMI） population W7984/Opata85. Significant and positive correlations were detected for all traits in this population except RLI. A total of 32 QTLs were associated with waterlogging tolerance on all chromosomes except 3A, 3D, 4B, 5A, 5D, 6A, and 6D. Some of the QTLs explained large proportions of the phenotypic variance. One of these is the QTL for GRI on 7A, which explained 23.92% of the phenotypic variation. Of them, 22 alleles from the synthetic hexaploid wheat W7984 contributed positively. These results suggested that synthetic hexaploid wheat W7984 is an important genetic resource for waterlogging tolerance in wheat. These alleles conferring waterlogging tolerance at early stages of growth in wheat could be utilized in wheat breeding for improving waterlogging tolerance.     

白桦次生林4个林龄0～30cm土层水源涵养功能比较

以小兴安岭地区白桦次生林4个林龄为研究对象,对其凋落物持水量、土壤贮水性能、土壤特性等进行研究.试验表明：4个林龄凋落物的蓄积量和最大持水量是70 a为最低,40 a为最大;土壤非毛管孔隙度在各林龄间变化呈波动性,30 a白桦次生林0～30 cm土层非毛管孔隙最小,不利于水分下渗,40 a白桦次生林0～ 30 cm土层非毛管孔隙最大,有利于降水的下渗,土壤水源涵养功能大小排序为40 a（3 276.820 t·hm-2）＜30 a（3 434.626 t·hm-2）＜50 a（3 525.015 t·hm-2）＜70 a（3 629.445 t·hm-2）.     

华北冬小麦开花及成熟期变化特征分析

利用华北平原59个农业气象观测站1981—2010年冬小麦生育期资料,分析了该区域冬小麦在气候变暖背景下开花期和成熟期的变化趋势特征。研究结果表明：近30年来,华北平原冬小麦开花期和成熟期均发生了明显变化。相对1980s而言,1990s开花期普遍提前2～5 d,成熟期提前1～6 d左右,2000s开花期则一般提前3～9 d,成熟期提前1～7 d左右。因此,随着年代推进,华北平原冬小麦开花期和成熟期提前趋势在进一步加剧。相对1980s而言,1990s生育期等值线普遍北移,而2000s等值线进一步北移的趋势更加明显。研究发现,3—5月月平均气温升高是开花期和成熟期提前的一个重要影响因素。     

不同施肥处理对湿加松早期生长及感病指数的影响

调查了福建省德化葛坑国有林场4种不同施肥处理后,2、3、4年生湿加松树高、胸（地）径和病害指数变化情况。结果表明,（1）不同施肥处理对湿加松早期树高、胸（地）径和松感病指数均产生显著影响,各生长年度树高、胸（地）径生长大小依次为：施氮磷钾复合肥与硼砂〉施氮磷肥与硼砂〉施氮磷肥〉对照（不施肥）,而感病指数大小则为：对照（...     

冀鲁豫夏玉米区玉米杂交种群体生育型演变──Ⅰ．演化阶段与演进基本途径

在实验基础上,从群体生育型演变进化角度分析了冀鲁豫夏玉米区六十年代初至九十年代初三十年间玉米杂交种种性提高的自然现象,明确了玉米杂交种群体生育型以产量行为与抗斑一倒行为的持续改良为基本途径演进。极端杂交种株粒重提高了４８．４％,叶斑指数与倒折率分别下降了４．３与１．２个百分点。演进过程界定为四个演化阶段：Ⅰ．六十年代,以新单１、白单４为代表;Ⅱ．七十年代前期,以群单１０５、丹玉６为代表;Ⅲ．七十年代后期至八十年代初,以郑单２、博单１为代表;Ⅳ．八十年代前期至九十年代初,以烟单１４和掖单２为代表。玉米杂交种群体生育型演变进化遵循“多元动态代谢结群,系统吐纳循序渐进”的生物进化规律。     

A plant ideotype for Bromus inermis Leyss. in Western Canada

Summary This study relates the yield of two harvests of Bromus inermis Leyss. to tiller type and morphology. Tillers were classified as being nonelongated, elongated and headed. At four times during the growing season, observations were made of tiller density; individual tiller weight; leaf number, area and weight; stem length, area and weight; leaf to stem ratio; specific leaf weight; head area and weight.Results showed that there was a pattern of tiller control over yield which was different for each of the two harvests. Stem characters contribute more to yield than leaves. Growth, early in the season, had a prominent effect on yield of the first harvest and also influenced the second harvest. Height at the time of each harvest was highly predictive of yield of the respective harvests. A morphological ideotype for bromegrass harvested twice in a season was described.     

新疆伊犁某牛场不同生长期牛粪源大肠杆菌耐药性调查

为了调查新疆伊犁某牛场不同生长期牛粪源大肠杆菌对临床常用抗菌药物的耐药情况。从该牛场分别采集育肥牛、繁育牛、加育牛和未繁育牛的粪样。采用微量肉汤稀释法,对分离出的大肠杆菌进行最小抑菌浓度测定。结果表明,育肥牛、繁育牛、加育牛和未繁育牛粪源大肠杆菌分离率均为100%,不同生长期的牛源大肠杆菌对氨苄西林均耐药,耐药率分别为4.0%、8.6%、6.7%、20.0%;对安普霉素都较为敏感。繁育牛和加育牛粪源大肠杆菌耐药种类较多,未繁育牛粪源大肠杆菌对比其他生长期牛粪源菌耐药较严重,不同生长期的牛粪源大肠杆菌多重耐药情况不太严重,主要为0耐、1耐;新疆伊犁该牛场对临床常用抗菌药物的耐药程度不同,耐药程度虽然不高,但在养殖生产中需注意合理用药。     

梅花鹿肉与绵羊肉在僵直化过程中主要理化性能的比较

通过对鹿肉与羊肉在宰后僵直化过程中保水性、pH值、游离羟脯氨酸、糖原、游离氨基酸等含量的检测及比较发现,鹿肉与羊肉在僵直化过程中各项理化指标变化趋势基本一致.鹿肉失水率、pH值、游离氨基酸含量比羊肉高(P＜0.01);游离羟脯氨酸含量比羊肉低(P＜0.01);巯基含量略高于羊肉,但差异不显著.鹿肉糖原含量略低于羊肉,但差异不显著.