1994～2002年山楂研究文献计量分析

对 1994～ 2 0 0 2年专业期刊公开发表的山楂文献做了计量分析。总文献量为 5 0 9篇 ,其中署名文献有 4 97篇 ,第一作者 374人 ,主要作者群为各类院校的教学科研人员 ,占 4 6 0 %。确定了 13位核心作者、10种核心期刊。     

杉木人工林立地质量的数量化评价

从21个立地因子中回归筛选出10个,与立地指数建立了数量化理论Ⅰ模型,并编制了开化杉木数量化立地指数得分表。分析结果表明,海拔、母岩、土壤厚度和土壤质地是影响开化杉木生长的主导立地因子。文中对这4个项目各类目组合而成的48个立地类型进行了数量化评价,预估出了各立地组合所潜在的生产能力。     

影响烟叶总糖和烟碱含量的土壤肥力因子评价

采用数量化理论Ⅰ评价影响烟叶总糖和烟碱含量的主要土壤肥力因子．结果显示，影响烟叶总糖量的主要土壤肥力因子是有机质、全Ｐ、速效Ｐ、缓效Ｋ和水溶性Ｂ；影响烟叶烟碱含量的主要土壤肥力因子有水溶性Ｂ、交换性Ｍｇ、缓效Ｋ和速效Ｐ     

Comparative QTL Mapping of Resistance to Gray Leaf Spot in Maize Based on Bioinformatics

The integration QTL map for gray leaf spot resistance in maize was constructed by compiling a total of 57 QTLs available with genetic map IBM2 2005 neighbors as reference. Twenty-six “real QTLs” and seven consensus QTLs were identified by refining these 57 QTLs using overview and meta-analysis approaches. Seven consensus QTLs were found on chromosomes 1.06, 2.06, 3.04, 4.06, 4.08, 5.03, and 8.06, and the map coordinates were 552.53, 425.72, 279.20, 368.97, 583.21, 308.68 and 446.14 cM, respectively. Using a synteny conservation approach based on comparative mapping between the maize genetic map and rice physical map, a total of 69 rice and maize resistance genes collected from websites Gramene and MaizeGDB were projected onto the maize genetic map IBM2 2005 neighbors, and 2 (Rgene32, ht1), 4 (Rgene5, rp3, scmv2, wsm2), and 4 (ht2, Rgene6, Rgene8 and Rgene7) positional candidate genes were found in three consensus QTLs on chromosomes 2.06, 3.04, and 8.06, respectively. The results suggested that the combination of meta-analysis of gray leaf spot in maize and sequence homologous comparison between maize and rice could be an efficient strategy for identifying major QTLs and corresponding candidate genes for the gray leaf spot.     

土壤肥力模糊定量化评价(判)系统的建立

本文根据系统方法的原理,选择多项土壤肥力因素指标为系统参数(包括土壤物理性质、土壤化学性质及土壤养分因素),应用模糊数学的决策方法和现代计算机技术,建立了土壤肥力的模糊定量化综合评判系统,FSQESF系统(A Fuzzy system for Quantitative E-valuation of Soil Fertility)FSQESF系统可以定量地评价土壤的实际肥力水平,提高土壤肥力评价的科学性和实用性,为土壤肥力监测与科学施肥提供了科学的依据和方法。     

陕西省生物防火林带树种选择研究

本文以燃烧热值、含水率、生物学特性、生态学特性和经济价值５个类目１７个项目为指标，对陕西省４１个预选树种进行排序和分类，选择出了３４个防火树种，其中优良防火树种４个，一般防火树种３０个；不适于作防火林带的易燃树种７个。     

DArTseq physical mapping of QTLs linked to Karnal bunt (Tilletia indica) resistance in two historical wheat populations

Karnal bunt, a disease of wheat, durum, rye, and triticale, is subject to strict quarantine restrictions worldwide. The disease is considered a major threat to food security, due to its use as a non-tariff trade barrier by several wheat-importing countries. In this paper, we analysed seven years of phenotypic data to search for quantitative trait loci (QTLs) associated with resistance in common wheat, validated the QTLs using an independent population, and assessed the potential of genomic selection as a tool for pre-emptive breeding. The QTL study used phenotypic data collected from artificially inoculated field experiments involving two historical Karnal bunt resistance populations: WH542 × HD29 and WH542 × W485. QTL analyses detected four significant (p < 0.001) QTLs on chromosomes 1A, 3A, 4B, and 6B, which explained between 13.7% and 15.7% of the phenotypic variation. A panel of 130 cultivars was used to validate QTL effects. These were genotyped with the same DArTseq protocol, and two of the four QTLs were significantly (p < 0.001) associated with Karnal bunt resistance in the validation panel. The potential of genomic selection was investigated by comparing accuracies of a model trained with all available markers and a model based solely on validated QTL information from the biparental populations. Genomic prediction accuracy, based on the two scenarios, averaged 0.43 and 0.33, respectively, suggesting that even in situations where phenotyping is difficult due to quarantine restrictions, the prospects for pre-emptive breeding against Karnal bunt are encouraging, and resources are now available that will reduce the cost burden.     

进境番茄种子中番茄花叶病毒的检测与鉴定

利用双抗夹心 酶联免疫吸附试验(DAS-ELISA)和反转录 聚合酶链式反应（RT-PCR）方法对来自韩国的番茄种子进行种传病毒检测。结果表明,在该批番茄种子中检测到番茄花叶病毒（ToMV）和烟草花叶病毒（TMV）。PCR产物测序结果表明,ToMV特异引物（ToA/ToB）与TMV特异引物（TA/TB）扩增的片段均为ToMV的外壳蛋白（CP）基因及3′非编码区（3′ UTR）,该片段与其他ToMV分离物的核苷酸序列相似性为98.2%~99.9%。本研究利用重新设计合成TMV和ToMV特异引物对该批种子进行RT PCR检测,仅ToMV特异性引物（ToMf1/ToMr1）扩增到670 bp的预期片段,TMV特异引物（TMf1/TMr1）则未出现特异性扩增,表明重新设计的引物可准确区分ToMV和TMV。以上结果证实该批番茄种子仅携带ToMV。