全文获取类型
收费全文 | 16268篇 |
免费 | 984篇 |
国内免费 | 1968篇 |
专业分类
林业 | 330篇 |
农学 | 1302篇 |
基础科学 | 199篇 |
993篇 | |
综合类 | 5914篇 |
农作物 | 1323篇 |
水产渔业 | 2008篇 |
畜牧兽医 | 5138篇 |
园艺 | 962篇 |
植物保护 | 1051篇 |
出版年
2024年 | 89篇 |
2023年 | 265篇 |
2022年 | 545篇 |
2021年 | 659篇 |
2020年 | 663篇 |
2019年 | 780篇 |
2018年 | 516篇 |
2017年 | 796篇 |
2016年 | 869篇 |
2015年 | 723篇 |
2014年 | 827篇 |
2013年 | 1076篇 |
2012年 | 1302篇 |
2011年 | 1223篇 |
2010年 | 1041篇 |
2009年 | 943篇 |
2008年 | 891篇 |
2007年 | 1025篇 |
2006年 | 813篇 |
2005年 | 632篇 |
2004年 | 481篇 |
2003年 | 404篇 |
2002年 | 380篇 |
2001年 | 348篇 |
2000年 | 254篇 |
1999年 | 242篇 |
1998年 | 155篇 |
1997年 | 158篇 |
1996年 | 136篇 |
1995年 | 154篇 |
1994年 | 98篇 |
1993年 | 102篇 |
1992年 | 97篇 |
1991年 | 87篇 |
1990年 | 92篇 |
1989年 | 92篇 |
1988年 | 50篇 |
1987年 | 49篇 |
1986年 | 34篇 |
1985年 | 21篇 |
1984年 | 19篇 |
1983年 | 8篇 |
1982年 | 11篇 |
1981年 | 10篇 |
1980年 | 10篇 |
1979年 | 9篇 |
1978年 | 5篇 |
1976年 | 7篇 |
1956年 | 12篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
82.
The paper studies a drug deliversy system for protein and find the optimum preparation condition.Cross-linked alginate(Alg) microcapsule was used to prepare microcapsule for protein,using bovine serum album(BSA) as a model drug.Effect of different cations and content of protein on configuration,size and enveloped rate of microcapsule were investigated.It is shown that Ba-Alg microcapsule has steady configuration,homogeneous size and high-enveloped rate.Also electrophoresis of native BSA before microencapsulation and after microencapsulation is measured by(SDS-PAGE),it is found that no significant change is observed BSA integrity.Release of BSA from microcapsule in vitro presents sustained release in some extent,and initial burst effect is significantly obvious.Microenpsulation drug in alginate will be a wishful drug delivery system. 相似文献
83.
Huang Wenzhen Huang Buyu Dong Xun 《保鲜与加工》1993,(1):72-77
Based upon the eigenvalue sensitivity theory of non-self-adjoit system and the component mode synthesis method,a new technique is developed to analyze the eigenvalue sensitivity to variation of parameters of large rotor-bearing system. As an application, the influence of the journal bearing parameters on the stability of a large domestic turbine-generator set is analyzed and discussed. 相似文献
84.
WANG Sheng~ 《保鲜与加工》2004,(7):36-39
Atomic Force Microscopy(AFM) plays a very important role in researching crystal growth mechanism, especially protein crystals. The principle of AFM for protein crystal examination is briefly described as well as the importance of research on protein crystal growth, known growth mechanisms including 2-D steps nucleation, spiral dislocation, normal growth, 3-D nucleation and anisotropic development, and latest progress about eucommia antifungal protein(EAFP) and Trichosanthin(TCS) protein crystal growth mechanism, respectively. Finally, protein growth under microgravity environment is also expounded here and their development tendency and practical prospect. 相似文献
85.
资源材料保存在作物遗传资源的研究与利用中起到非常重要的作用。该实验以不同来源的人工合成甘蓝型油菜KH106,KH109,KH111D为材料,以花序轴,子房,果柄为外植体研究其再生情况,结果表明,由芥蓝和小白菜人工合成的甘蓝型油菜KH106的再生频率极显著高于由羽衣甘蓝和白菜型油菜人工合成的甘蓝型油菜KH109和KH111;与花序轴和子房为外植体再生频率相比,以果柄为外植体平均再生频率最高,差异达到极显著水平;利用不同预处理培养基处理不同时间,结果显示不经过预处理直接接种到分化培养基上再生频率最高。 相似文献
86.
SU Ying JIN Chun SUN Su-min LI Zhang-hao XIA Si-wei ZHANG Zi-li ZHANG Feng SHAO Jiang-juan ZHENG Shi-zhong 《园艺学报》2021,36(12):2283-2288
RNA-binding proteins (RBP) are molecules with a variety of biological functions discovered in recent years. Among them, HuR is an important RBP, widely expressed in various tissues of the body, and is a member of the Hu/embryonic lethal abnormal vision (ELAV) protein family. It mainly affects the expression levels of target genes in the cells by regulating the stability and/or translation efficiency of the mRNA of the genes, thus participating in the regulation of cell life activities. In recent years, more and more studies on HuR have revealed its important role in inflammation and cancer. This review summarizes the functions of HuR and probes into the regulation of HuR functions. In addition, the roles of HuR in the occurrence and development of hepatocellular carcinoma (HCC) are also discussed, which provides important information for exploring the pathogenesis, biomarkers and therapeutic targets of HCC. 相似文献
87.
88.
The strategy and synthetic efforts leading to efficient stereoselective syntheses of thiangazole, a tris-thiazolinyl-oxazole metabolite isolated from Polyangium spp., and of hydantocidin, a spironucleoside metabolite isolated from Streptomyces hygroscopicus, are discussed. 相似文献
89.
布鲁菌外膜蛋白及毒力因子研究进展 总被引:2,自引:0,他引:2
布鲁菌细胞膜的基本结构包括脂多糖和外膜蛋白,与细菌的毒力及免疫原性相关。文章描述了布鲁菌外膜蛋白分子结构的最新进展。该菌的外膜蛋白由第一组、第二组和第三组外膜蛋白构成。第一组外膜蛋白对维持布鲁菌外膜蛋白的结构起重要作用;第二组包括36 ku~38 ku外膜蛋白,为膜孔蛋白,由Omp2a和Omp2b基因编码,其中38 ku蛋白基因可能是一个与毒力相关的基因;第三组外膜蛋白包括31 ku和25 ku两个相关的蛋白,具有重要的免疫功能。31 ku蛋白属膜孔蛋白,25 ku蛋白还与毒力有关。文章也介绍了布鲁菌毒力因子研究的最新进展。 相似文献
90.
Marine Remize Frdric Planchon Matthieu Garnier Ai Ning Loh Fabienne Le Grand Antoine Bideau Christophe Lambert Rudolph Corvaisier Aswani Volety Philippe Soudant 《Marine drugs》2022,20(1)
The production of polyunsaturated fatty acids (PUFA) in Tisochrysis lutea was studied using the gradual incorporation of a 13C-enriched isotopic marker, 13CO2, for 24 h during the exponential growth of the algae. The 13C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in T. lutea. The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in 13C. On the contrary, 18:4n-3 and 18:5n-3 were the least enriched in 13C after long chain polyunsaturated fatty acids such as 20:5n-3 or 22:5n-3. The algae appeared to use different routes in parallel to form its polyunsaturated fatty acids. The use of the PKS pathway was hypothesized for polyunsaturated fatty acids with n-6 configuration (such as 22:5n-6) but might also exist for n-3 PUFA (especially 20:5n-3). With regard to the conventional n-3 PUFA pathway, Δ6 desaturation of 18:3n-3 appeared to be the most limiting step for T. lutea, “stopping” at the synthesis of 18:4n-3 and 18:5n-3. These two fatty acids were hypothesized to not undergo any further reaction of elongation and desaturation after being formed and were therefore considered “end-products”. To circumvent this limiting synthetic route, Tisochrysis lutea seemed to have developed an alternative route via Δ8 desaturation to produce longer chain fatty acids such as 20:5n-3 and 22:5n-3. 22:6n-3 presented a lower enrichment and appeared to be produced by a combination of different pathways: the conventional n-3 PUFA pathway by desaturation of 22:5n-3, the alternative route of ω-3 desaturase using 22:5n-6 as precursor, and possibly the PKS pathway. In this study, PKS synthesis looked particularly effective for producing long chain polyunsaturated fatty acids. The rate of enrichment of these compounds hypothetically synthesized by PKS is remarkably fast, making undetectable the 13C incorporation into their precursors. Finally, we identified a protein cluster gathering PKS sequences of proteins that are hypothesized allowing n-3 PUFA synthesis. 相似文献